Ectomycorrhizal dynamics of white fir stands at Teakettle

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Ectomycorrhizal dynamics of white fir stands at
Teakettle
A.D. Izzo and T.D. Bruns Plant and Microbial
Biology University of California, Berkeley
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Questions
what is the community structure of
ectomycorrhizal fungi associated with white
fir? how does species richness and diversity
change following fire/thinning? what is the
role of the spore bank in these scenarios?
how does the spore bank respond to heat/ash?
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Sampling
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5
10
20
White fir stand
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ID of root tips by RFLP matches
Techniques RFLP matching
separate morphotypes freeze dry, weigh
Species ID
match
PCR RFLP
PCR RFLP
DNA sequence (ITS)
Taxonomic ID (Phylum, Family, Genus, RFLP
taxon)
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ECM biomass highly variable
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BK
AB
Basidio18
Thelephoroid2
Piloderma2
Piloderma
Russuloid1
Russulaceae
Basidiomycete
Russula
Agaricales
Cortinarius
Cortinariaceae
Cortinarioid1
Pezizales
Otideaceae
Wilcoxina
Wilcoxina1
Ascomycete
Cencococcum
Cencococcum3
RFLP-taxon
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Note minimal temporal similarity
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Comparison of treatments
Minimal overlap of dominant RFLP-taxa
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Family-level comparison
TK99
TK01/99 (2 yrs)
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ECM islands of dominance
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2
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Additional studies
Spatial bioassays - soils from all 54 plots
pre-treatment - ABCO, PIJE, ABCO PIJE
Heat and Ash bioassays Pooled soil from 7-8
mature white fir stands across Teakettle C, 40,
55, 70 deg C, ash ABCO, PIJE
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Acknowledgements
Molecular analysis Josephine Agbowo
Collectors 1999/2001 Martin Bidartondo Ryan
Bowman Francisco Camacho Lisa Grubisha Erik
Lilleskov
Bioassay setup Kari Cheng
Funding USDA, PMB dept