Hypothesis

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Hypothesis

• Combination of three transcription factors (NGN3,
  PDX1 and MaFa) enable to reprogram differentiated
  pancreatic exocrine cells in adult mice into
  cells that closely resemble ß cells.

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Methods
Nine transcriptional factors were exclusively
selected NKx2.2, Nkx6.1, and Pax4 were first
group to be excluded, Ngn3, Pdx1 and Mafa were
identified as critical factors to be able to
reprogram exocrine cells in pancreas to become
insulin production cells within three days after
transfect with adenovirus. Evaluation methods
Brdu labeling TUNEL assay multiple labeled
immunohistochemistry Cre-Loxp cell tracing
technology
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Results
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A Combination of Three Transcription Factors
Induces Insulin Cells in Adult Mouse Pancreas in
Vivo
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Induced New B-Cells Originate From Differentiated
Exocrine Cells
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Endogenous Induced B-Cells Are
Indistinguishable In Morphology Ultrastructure
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Molecular Marker Characterization of Induced B
Cells
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Induced New B-Cells Remodel Vasculature
Ameliorate Hyperglycaemia
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Conclusion

• This study provides an example of cellular
  reprogramming using defined factors in an adult
  organ and suggests a general paradigm for
  directing cell reprogramming without reversion to
  a pluripotent stem cell state.