Fluorochrome TB Smear Microscopy, Culture and Drug Susceptibility Testing

1
Fluorochrome TB Smear Microscopy, Culture and
Drug Susceptibility Testing

• Ruth Tembwe
• Manager, Chest Diseases Laboratory

2
Workshop Objectives

• Prepare reagents, perform and report flourochrome
  AFB smear microscopy
• Correlate ZN and fluorochrome AFB smear
  microscopy
• Prepare reagents and media for detection of
  mycobacteria and susceptibility testing
• Explain safety measures for culture and DST

3
Workshop Objectives

• Report culture and drug susceptibility test
  results for M. tuberculosis
• Perform biochemical tests and identify M.
  tuberculosis
• Susceptibility testing of bacteriology in the
  routine microbiology
• Document and perform internal quality control and
  EQA for smear microscopy, culture and DST of
  M.tuberculosis and other bacterial pathogens in
  clinical specimens