Consortium for Functional Glycomics Pamela A. Marino, Program Director

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Consortium for Functional GlycomicsPamela A.
Marino, Program Director
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Consortium for Functional Glycomics
http//www.functionalglycomics.org
Define paradigms by which protein-carbohydrate
interactions mediate cell communication

• NIGMS sponsored 'glue grant' (8 MM/yr) most
  in cores.
• Over 250 participating investigators worldwide
• Focus in innate and acquired immunity

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Consortium for Functional Glycomics
Steering Committee
Consortium Databases MIT
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Structure of the Alliance
NCI Tumor Glycome Laboratories
NIGMS CFG Cores Databases Analytical
Chemistry Synthetic Chemistry GlycoT Gene
Arrays Glycan Arrays
NCI EDRN Components DMCC
Coordination Unit
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Consortium for Functional Glycomics
Glycobiology Resources Offered
Glycan array screening
Glyco-gene microarry
Glycan specificity of your carbohydrate binding
protein on an array with 300 unique glycans
representing glycoprotein and glycolipid glycans
Analysis of your RNA samples on a highly
annotated custom gene microarray with 1800 human
and mouse genes
Mouse knockout strains
Carbohydrate compounds
30 mouse strains with genes ablated for
glycosyltransferase and glycan-binding proteins
Over 100 carbohydrate compounds represented on
the glycan array are available with azido or
biotin functional groups
http//www.functionalglycomics.org
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Analytical Glycomics Core

• Human Glycomics MALDI profiling of N- and
  O-glycans of tissues, primary cells.
• Murine (and human) glycan profile data
  disseminated on website.

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Glycan Array Core

• Printed Glycan array expanded to 300 glycans.
• Perform Analysis of glycan binding proteins.
• Results updated regularly on website.
• .

SNA
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Focused Glycogene Microarray

• GLYCOv3 chip with up to date gene list highly
  annotated by participating investigators
• Genes for Glycosyltransferases Glycan-binding
  proteins,Glycan degradation proteins Notch
  pathway proteins Nucleotide sugar synthesis and
  transporter proteins N-glycan biosynthesis-relate
  d proteins Adhesion molecules Interluekins and
  receptors Mucins Growth factors and receptors
  Cytokines Chemokines Conserved oligomeric Golgi
  (COG) complex proteins
• Data entered into database and posted on website.

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Mouse Transgenics / Phenotyping Cores

• Over 30 KO lines available
• Phenotypic data on website

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CFG DATABASES

• Glycan Binding Protein Database Molecule pages
  for 150 glycan-binding proteins
• Glycan Structural Database Over 7500 entries for
  N-linked, O-linked and synthetic glycans contain
  structural and chemical information, as well as
  PubMed links to the source articles.
• Glycosyltransferase Database Glycosylation
  pathways pages for different glycoconjugate
  classes. Arrows between structures link to the
  appropriate CAZY database page for the GT.
• power point demo of data bases here
  http//www.functionalglycomics.org/static/consorti
  um/CFG_Bioinformatics_Progress_2006.ppt