Diversity of Streptococcal Pathogens in Throat Infections of Students on the Stetson University Campus Stephanie Wisniski

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Diversity of Streptococcal Pathogens in Throat
Infections of Students on the Stetson University
CampusStephanie Wisniski
Research performed at Stetson Universitys
Department of Biology in conjunction with Student
Health Services
Example of DNA barcode
Picture supplied by Bacterial Barcodes, Inc.
Electrophoresis gels of PCR DNA fragments from
university students
Sample116
Negative Control
Negative Control
Positive Control
Positive Control
Sample138
Sample 103
Sample 100
Sample 126
Sample 139
Sample 105
Sample 130
Sample 124
Sample 111
Sample 107
Sample 123
Ladder
Ladder
Ladder
Ladder
wells?
Results

• No DNA fragment patterns are identical in
  patients sampled having streptococcal infections.

Gel 2
Gel 1
Table 1. Certain components of the ladder in gel
1 indicate similarities when comparing samples to
one another. Table 2. Certain components
of the ladder in gel 2 indicate similarities when
comparing samples to one another.
Similar but not identical barcodes?
Sample number
Yes
124 Pos.Control
Yes
130 124
Yes
124 103
Similar but not identical barcodes?
Sample number
Yes
139 Ladder
Yes
138 Ladder
Yes
138 100