Supervisory Responsibility and Guidelines for a BSL3 Laboratory

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Supervisory Responsibility and Guidelines for a
BSL-3 Laboratory
Dustin C. Yang., Ph.D. Centers for Disease
Control, Taiwan October 7, 2008
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Pre-Test 1
Q
Which one is the correct symbol for Biohazard?
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Pre-Test 2
Q
The Biohazard symbol must be affixed to any
container or equipment used to store or transport
potentially infectious materials.
True False
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Pre-Test 3
Q
All materials (incubators, centrifuges,
pipettors,etc) which come in contact with
radioactive materials must be labeled with this
radiation symbol!
True False
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WHO Laboratory Biosafety Manual 3rd ed. 2004
Part VII Safety Organization and Training
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Responsibilities of Biosafety Officer (BSO) -1

• Biosafety, biosecurity and technical compliance
• consultations.
• Periodic internal biosafety audits on technical
  
• methods, procedures and protocols,
  biological agents, materials and equipment.
• Discussions of violation of biosafety protocols
  or procedures with the appropriate persons.
• Verification that all staff have received
  appropriate biosafety training.
• 5. Provision of continuing education in
  biosafety.

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Responsibilities of Biosafety Officer (BSO)- 2
6. Investigation of incidents involving the
possible escape of potentially infectious or
toxic material, and reporting of findings
and recommendations to the laboratory
director and biosafety committee. 7. Coordination
with medical staff regarding possible
laboratory-acquired infections. 8. Ensuring
appropriate decontamination following spills
or other incidents involving infectious
material(s). 9. Ensuring proper waste management.
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Responsibilities of Biosafety Officer (BSO)- 3
10. Ensuring appropriate decontamination of any
apparatus prior to repair or servicing. 11.
Maintaining awareness of community attitudes
regarding health and environmental
considerations. 12. Establishment of appropriate
procedures for import/export of pathogenic
material to/from the laboratory, according
to national regulations. 13. Reviewing the
biosafety aspects of all plans, protocols
and operating procedures for research work
involving infectious agents prior to the
implementation of these activities. 14.
Institution of a system to deal with emergencies.
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Supervisory Responsibility

• All managers and supervisors are
• responsible for the health and safety of their
  staff, guest researchers, visiting scientists,
  contractors, and visitors.

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• Safety Incident 1
• I was standing on top of 2 full boxes (18) to
  reach a larger
• box full of binders which was on top of a
  bookcase. With the
• box of binders balanced on my shoulder, I
  stepped off of the
• boxes and lost my balance when the step was
  farther than
• I thought. As I lost my balance, the box of
  binders slammed into my cheekbone. I tossed them
  away, landed on the back of my heel, and then
  fell onto my right hip and back. I then rolled
  backwards hitting my spine, shoulders, and head.

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• Safety Incident 2
• Complains of pain in wrists from resting hands
  on computer tray. ...
• Complains of pain in left wrist and forearm
  which is believed to be caused by computer mouse
  usage or typing on keyboard. ...
• The day after I did a lot of typing, I lost use
  of my right hand and wrist, making it hard to
  type, open tops, or lift heavy objects.

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• Safety Incident 3
• A needle was being held by a co-worker which had
  just been used on a ferret showing clinical signs
  of rabies. When he handed it to me, I was stuck
  in the hand.

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Safety Incident 4 I was cleaning paper out
of drain in the sink with gloves on when I was
pricked by a piece of glass on the left thumb.
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Safety Incident 5 Laboratorian is heating ethyl
ether in a beaker on a hot plate in a fume hood.
As more liquid ether is being added to the
beaker, the hot plate thermostat engages and
ignites the ether fumes.
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Safety Incident 6 I was working with infant
mice infected with Strep. pneumoniae, trying to
draw blood from the tail vein with a monolet
lancet. I pushed down too hard and it went
through the tail and into the nuckle of my left
middle finger.
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Safety Incident 7 Laboratorian is working alone
with Neisseria meningitis sp, concentrating it
using a bench-top centrifuge prior to alioquoting
into cryovials and final freezer storage.
Several days later, laboratorian develops
extremely high fever and chills, reports to
emergency room, is admitted, and comes within two
hours of dying from agent they were working with.
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Safety Incident 8 Laboratorian is streaking agar
plates with suspected multi-drug resistant
organism. Laboratorian wakes during the night in
severe gastric distress is admitted to hospital
and treated for several days testing reveals
agent they worked with. Investigation reveals
probable contamination of lab coat cuff during
plating a ctivities followed by
wrist/hand contamination.
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Safety Incident 9 Laboratorian was spinning
Sabia arenavirus when the tube broke. He opened
the centrifuge, cleaned up the spill but did not
notify anyone of the incident. 24 days later, the
laboratorian reports to hospital with 1030
temperature is admitted, and diagnosed with
Sabia arenavirus infection. He now notifies
authorities of previous incident and eighty
people were subsequently monitored for the
disease.
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Safety Incident 10 Waste disposal technician
retrieves discard pan from Rocky Mountain spotted
fever lab. He thought it had been autoclaved and
begins to clean it out using a high pressure
hose. A friend comes by to pass the time of day.
Several days later, both men develop high fevers,
report to emergency room and are admitted. Both
die of complications of their illness and testing
reveals infection with Rocky Mountain spotted
fever..
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Safety Incident 11 After injecting the chimp,
the animal tech did not immediately dispose of
the injection needle. When he turned to assist
the animal caretaker in releasing the animal from
the squeeze cage, the caretaker was stuck in the
right, 4th finger. The chimp was chronically
infected with hepatitis C and G.
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Safety Incident 12 After adding 10 ml of conc.
sulfuric acid to a mixture of 260 ml of acetic
anhydride and 130 ml of acetic acid, I realized
that I had made a mistake. As I turned to discard
the mixture into a container by the sink, I
tripped over a chair, spilling the contents into
the sink. The acid immediately reacted with water
in the sink and back-splashed, onto my body.
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Safety Incident 13 Laboratorian opens
refrigerator door and an unlabeled bottle falls
from door and breaks on floor. Volatile chemical
immediately fills room with noxious fumes
building is evacuated no one IDs the bottle.
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Safety Incident 14 Delivery person drops a box
containing two 4-liter bottles of xylene in the
atrium of building 15, breaking one of the
bottles. Delivery person leaves the scene without
notifying anyone. Several laboratorians walk by,
over, and around the spill before calling OHS to
clean up the spill.
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Do you know your responsibilities as a supervisor?

• 1. Are you familiar with the Infectious Disease
  Control Act , rules, and regulations that apply
  to the work under your supervision?
• 2. Do you understand the risks and hazards of the
  work under your supervision?
• 3. Are you clear about whom and what area you are
  responsible as a supervisor?
• 4. Do you understand the health and safety
  responsibility and authority that have been
  delegated to you?
• 5. Do you routinely inspect your work areas,
  getting to the cause and correction of problem
  areas?

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Do you know your responsibilities as a supervisor?

• 6. Do you encourage workers to report health and
  safety
• problems and not to cover up mistakes?
• 7. Do you investigate incidents and accidents o
  prevent future recurrence?
• 8. Do you ensure that new workers, or workers new
  to the job, are trained before they begin the
  task?
• 9. Do you provide refresher training as necessary
  to prevent non-compliance in practices and
  procedures?
• 10. Do you keep records of training and problem
  resolution?

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Do you know your responsibilities as a supervisor?

• 12. Do you enforce the use of required protective
  equipment
• and adherence to safety policies and
  procedures in work
• areas?
• 13. Have you attended the health and safety
  training that is
• required for a supervisor and for the
  employees under your
• charge?
• 14. Do you review the required documents in your
  work area to
• ensure they are up to date? E.g. MSDSs,
  chemical
• inventory, policies, programs, and training
  records?

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Biosafety Training-I. General Safety

• Back Safety
• CPR-Adult
• Ergonomics Awareness
• First-Aid
• Hazard Communication
• Hearing Protection

• Occupant Emergency Plan Training
• Respiratory Protection
• Safe use of Fire Extinguisher
• Safety and Health Orientation
• Supervisory Responsibility
• Animals in Research

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Biosafety Training-II.Laboratory Safety

• Biological Safety
• Bloodborne Pathogens
• Gamma-cell Radiation
• Laboratory Safety
• Radiation Safety in the Laboratories
• Hazardous Material Transportation
• Hazardous Chemical Waste Generator

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Biosafety Training- III.Chemical and
Environmental Safety

• Hazardous Material Database Training
• Asbestos Awareness
• Asbestos Abatement Worker

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Biosafety Training- IV.Construction Safety

• Refrigerants
• Lockout/Tagout
• Permit-required Confined Space

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Employee Responsibilities

• 1. Comply with occupational safety and health
  standards, rules, regulations, and orders issued
  by DOH and CDC.
• 2. Use personal protective equipment and safety
  equipment when needed.
• 3. Report all work related accidents and illness
  to their supervisors.

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Supervisory Responsibilities

• 1. Promote and support positive safety and health
  behaviors and practices among employees.
• 2. Prevent employee injury/illness and provide
  assistance to those seeking treatment for
  injuries and/or illnesses.
• 3. Investigate and report all accidents and/or
  injuries to the Office of Health and Safety.
• 4. Ensure that all workers know and follow safe
  work procedures promptly correct unsafe
  practices and/or actions.
• 5. Ensure that employees are aware of their
  responsibilities and work in safe, uncluttered
  surroundings.
• 6. Ensure that all equipment operates safely and
  per manufacturer specifications

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Supervisory Responsibilities

• 7. Ensure that all employees know the location
  and operation of emergency equipment (first aid
  kits, eyewash/shower stations, fire
  extinguishers/blankets, chemical spill kits) and
  personal protective equipment (lab coats, gloves,
  gowns, eyewear).
• 8. Conduct regular discussions, surveys, and
  demonstrations of safe work practices and
  emergency response.
• 9. Monitor safety training needs of all employees
  including visitors provide training
  opportunities in conjunction with CDC/Taiwan.
• 10. Comply with all applicable occupational
  health and safety standards, rules and
  regulations as well as specific CDC/Taiwan
• criteria.