Microbial Growth

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Chapter 6

• Microbial Growth

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Requirements for growth

• Physical requirements
• Temperature
• Psychrophiles
• Mesophiles
• Thermophiles
• Psychrotrophs

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Requirements for growth

• Physical requirements
• Temperature
• pH
• Osmotic pressure
• Halophiles

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Requirements for growth

• Physical requirements
• Chemical requirements
• Carbon
• Nitrogen
• Sulfur
• Phosphorus
• Trace elements

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Requirements for growth

• Physical requirements
• Chemical requirements
• Oxygen
• Aerobes
• Anaerobes
• Facultative
• Microaerophilic
• Aerotolerant

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Culture media

• Chemically defined media

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Culture media

• Chemically defined media
• Complex media

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Culture media

• Chemically defined media
• Complex media
• Anaerobic growth media and methods

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Culture media

• Chemically defined media
• Complex media
• Anaerobic growth media and methods
• Growth of capnophiles

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Culture media

• Chemically defined media
• Complex media
• Anaerobic growth media and methods
• Growth of capnophiles
• Selective and differential media

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Growth media

• Chemically defined media
• Complex media
• Anaerobic growth media and methods
• Growth of capnophiles
• Selective and differential media
• Enrichment media

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Obtaining pure cultures

• Streak plate
• Pour plate

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Preserving cultures

• Freezing
• Freeze drying
• Drying

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Growth of bacterial cultures

• Bacterial division

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Growth of bacterial cultures

• Bacterial division
• Generation time
• Phases of growth
• Lag phase
• Log phase
• Stationary phase
• Decline phase

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Measurement of microbial growth

• Plate counts and serial dilution
• Direct microscopic count

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Measurement of microbial growth

• Plate counts and serial dilution
• Direct microscopic count
• Filtration

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Measurement of microbial growth

• Plate counts and serial dilution
• Direct microscopic count
• Filtration
• Most probable number

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Measurement of microbial growth

• Plate counts and serial dilution
• Direct microscopic count
• Filtration
• Most probable number
• Estimating numbers
• Turbidity
• Titratable acidity

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