Firefly luciferase gene marking for in vivo Bioluminescence Imaging BLI

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Firefly luciferase gene marking for in vivo
Bioluminescence Imaging (BLI)

Firefly Luciferase
Emitted photons
Quantitative measurement of Bioluminescence using
a sensitive camera system located in the
infection unit of the GDL/UU

• BLI can be used to visualize luciferase gene
  expression in
• Transgenic animals
• To study homing/engraftment and outgrowth in
  normal animals of
• Hematopoietic Stem Cells
• Leukemia cells
• Mesenchymal Stem Cells
• Lymphocytes
• Tumor cells and to measure the effect of
  cyto-reductive treatment
• with drugs or monoclonal antibodies
• To visualize luc gene expression in
• Bacteria, virusses

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Example Luc marked normal murine BM cells
? photonluc activity cell load
Luciferin i.p.
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Liver metastasis model (oncology)
injection of CC cells intra spleen spleenectomy
Data Dr. Niels Smakman, DIGD/Oncology UMCU
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Correlation of Hepatic replacement area (HRA)
with fLuc activity
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In vivo imaging of fLuc transduced mesenchymal
stem cell expansion on hydroxyapatite scaffolds
for bone production
Control
Cultured
Non-cultured
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Example of human Multiple Myeloma cell growth in
immune deficient mice
Without BLI this unpredictable tumor growth could
not be measured
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Multiple Myeloma model
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Info A.C. Martens Dept Immunology
UMCU a.martens_at_umcutrecht.nl/ 030-250-4009