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Lab Exercise 14:

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Beta ( ) hemolysis (blood agar around bacterial ... Staphylococcus epidermidis. Enterococcus faecalis. G. M. Who am I? ... Answer: Staphylococcus aureus. M. G ... – PowerPoint PPT presentation

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Title: Lab Exercise 14:


1
Lab Exercise 14
Biochemical Differentiation of some Medically
Important Gram Positive Cocci
2
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3
Beta ( ß ) hemolysis (blood agar around bacterial
colonies is completely clear, indicating complete
breakdown and consumption of hemoglobin)
4
Alpha (a) hemolysis (blood agar around bacterial
colonies looks greenish-brown, indicating partial
breakdown and consumption of hemoglobin)
Mixed culture from a throat swab note several
ß-hemolytic colonies
5
Gamma (?) hemolysis (really means no hemolysis
blood agar around colonies looks normal)
6
Side-by-side comparisons of alpha (a), beta (ß),
and gamma (?) hemolysis
7
Bacitracin (Taxo A) sensitivity
results with a zone of inhibition
8
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10
Coagulase Test

-
11
Carbohydrate Fermentation Tests (Glucose and
Mannitol)
- (same as uninoculated media)

Phenol red indicator turns yellow at acid pH
12
Catalase Test
13
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14
Use the chart on p. 146 and/or the flow chart on
p. 147 of your lab manual to figure out who I am!
Answer Streptococcus pyogenes
15
Use the chart on p. 146 and/or the flow chart on
p. 147 of your lab manual to figure out who I am!
G
M
Answer Micrococcus lutea
16
Use the chart on p. 146 and/or the flow chart on
p. 147 of your lab manual to figure out who I am!
A
Answer Enterococcus faecalis
17
Answer Staphylococcus aureus
18
Use the chart on p. 146 and/or the flow chart on
p. 147 of your lab manual to figure out who I am!
Answer Staphylococcus epidermidis
19
Use the chart on p. 146 and or the flow chart on
p. 147 of your lab manual to figure out who I am!
Answer Streptococcus pneumoniae
20
Use the chart on p. 146 and/or the flow chart on
p. 147 of your lab manual to figure out who I am!
A
Answer Streptococcus mutans
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