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MICHAEL HOOKER MICROSCOPY

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BOTANY DEPARTMENT Light Microscopy, Confocal, Nina Allen, Box 7612, NCSU ... Use Animal Model systems for refining specific imaging technologies i.e. ... – PowerPoint PPT presentation

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Title: MICHAEL HOOKER MICROSCOPY


1
MICHAEL HOOKER MICROSCOPY
  • Core Facilityopen to all UNC investigators
  • Confocal fluorescence/reflection/frap/fret
  • Atomic Force Microscope
  • Laser Micro-Dissection
  • Light Microscopy tans./fluor./DIC/phase/multimod
    e/time-lapse
  • http//mhmicroscopy.med.unc.edu
  • Mike Chua 843-3268

2
MICROSCOPY SERVICES
  • UNC Core facility
  • Confocal
  • Laser Capture Microscopy
  • Electron Microscopy
  • Light Microscopy
  • http//www.med.unc.edu/microscopy/
  • Bob Bagnell 966-2413

3
FLOW CYTOMETRY CORE
  • UNC Core Facility
  • Deconvolution System (3D)
  • Laser Scanning Cytometer
  • FACS
  • http//flowcytometry.med.unc.edu/
  • Larry Arnold 966-1530

4
R. SUPERFINE - T. SALMON LABS
  • Scanning Electron Microscopy
  • Transmitted Electron Microscopy
  • Atomic Force Microscopy
  • 3D Force Microscopy
  • Confocal Microscopy
  • Light Microscopy
  • rsuper_at_physics.unc.edu
  • tsalmon_at_email.unc.edu

5
RUSSELL TAYLOR- VISUALIZATION LAB
  • Scientific Visualization
  • Distributed Virtual Worlds
  • Haptic Display
  • Interactive 3D Computer Graphics
  • UNC NIH National Research Resources for Computer
    Integrated Systems for Microscopy and
    Manipulation--
  • http//www.cs.unc.edu/Research/nano/cismm/

6
UNC NEUROIMAGING LABORATORYG. Gerig, A. Belger,
J. Lieberman, J. Piven
  • Cognitive Neuroscience
  • ERP lab Functional brain mapping
  • Functional brain mapping using fMRI
  • Analysis of MRI diffusion tensor imaging (DTI)
  • 3D ultrasound Quantitative image analysis
  • Developmental Neuroimaging UNC-NDRC Core
  • High-power computer system dedicated to
    neuroimage analysis
  • Neurolab http//zeus.ia.unc.edu/
  • NIRL http//www.nirl.unc.edu/
  • NDRC http//www.fpg.unc.edu/ndrc/cores.cfm

7
BARRY WHITSEL LAB
  • Intrinsic Optical Signal Imager (cell-based, in
    vivo)
  • bwhitsel_at_med.unc.edu

8
BIOLOGY Kerry Bloom Ted Salmon Tony Perdue
  • Spinning disk Confocals
  • Laser Scanning Confocals
  • Transmission Electron Microscopy
  • kerry_bloom_at_unc.edu
  • tsalmon_at_email.unc.edu
  • tdperdue_at_email.unc.edu

9
BIOMEDICAL ENGINEERING
  • MRS Metabolomics Imaging (J. MacDonald)
  • Cortical Intrinsic Signal Imaging
  • Multiphoton fluorescence Imaging
  • Transmembrane Potential Imaging
  • Intracellular Calcium Imaging
  • Biophotonics Computer Imaging
  • macdona1_at_email.unc.edu

10
CELL DEVELOPMENTAL BIOLOGY J. LeMasters J.
Costello K. Jacobson
  • Confocal Single 2 Photon
  • Cryo Transmitted Electron Microscopy
  • Scanning Electron Microscopy
  • Optical Tweezers
  • Fluorescence Recovery After Photobleaching
  • lemaster_at_med.unc.edu
  • mjc_at_med.unc.edu
  • frap_at_med.unc.edu

11
CHEMISTRY
  • Nuclear Magnetic Resonance
  • Spectroscopy
  • Total Internal Reflection Microscopy Nancy
    Thompson
  • nit_at_unc.edu

12
DENTAL SCHOOL
  • Transmitted Electron Microscopy
  • Scanning Electron Microscopy
  • Wallace Ambrose
  • wallace_ambrose_at_dentistry.unc.edu

13
PEDIATRICS/EPAJ. CARSON
  • Freeze Fracture
  • Transmitted Electron Microscopy
  • Scanning Electron Microscopy
  • Light Microscopy
  • jlcarson_at_email.unc.edu

14
RADIATION ONCOLOGYEdward Chaney
  • CT
  • Segmentation
  • Registration
  • 3D 4D Display
  • chaney_at_med.unc.edu

15
RADIOLOGY RESEARCHWeili Lin Etta Pisano
Stephen Aylward
  • 3 Tesla Magnetic Resonance Imaging (Lin)
  • Micro Single-Photon Computed Tomography (Lin)
  • Diffraction Enhanced Imaging(Pisano)
  • Computer Aided Diagnosis and Display Lab
    (CADDLab Aylward)
  • -- 2D and 3D image segmentation and registration
  • --Multi-modal image resgistration
  • weili_lin_at_med.unc.edu etta_at_med.unc.edu
  • aylward_at_unc.edu

16
COMPUTER-ASSISTED SURGERY IMAGING LAB
(CASILAB)Elizabeth Bullitt
  • Vessel/Tumor
  • Imaging and Analysis
  • Imaged Guided
  • Surgery
  • bullitt_at_med.unc.edu

17
GENE THERAPY CENTER
  • Optical Imaging (Cryocooled, luminescence and
    fluorescence)
  • Allison Hawke
  • allison_hawke_at_med.unc.edu

18
LINEBERGER COMPREHENSIVE CANCER CENTER
  • Light Microscopy
  • Fluorescence Microscopy
  • Steve Oglesbee
  • big_rock_at_email.unc.edu

19
NEUROSCIENCES NEURODEVELOPMENTAL DISORDERS
RESEARCH CENTERS
  • Eva Anton - Robert Sealock
  • Confocals Single 2-Photon
  • Light Microscopy
  • sealock_at_med.unc.edu
  • anton_at_med.unc.edu

20
VASCULAR BIOLOGY CENTER Cam Patterson
  • Ultrasound for small animals
  • cpatters_at_med.unc.edu

21
MEDICAL IMAGE DISPLAY ANALYSIS GROUP (MIDAG)
  • At Comp Sci, Psychiatry, Rad Onc, Radiology,
    Surgery
  • Analysis Capabilities
  • Image Analysis
  • Image Display
  • Augmented Reality (Computer Science)
  • Segmentation
  • Registration
  • http//midag.cs.unc.edu

22
NC STATE UNIVERSITY
  • BOTANY DEPARTMENT Light Microscopy, Confocal,
    Nina Allen, Box 7612, NCSU
  • John MacKenzie Coordinator, EM Center
    john_mackenzie_at_ncsu.edu
  • Phillip Russell Dir, Analytical Instru.
    Facility Atomic Force TEM, prussell_at_ncsu.edu
  • Michael Dykstra Dir, Laboratory Advanced
    Microscopy, TEM Confocal michael_dykstra_at_ncsu.ed
    u
  • VET SCHOOL X-ray, CT, ultrasound, MRI
  • Ian Robertson, ian_robertson_at_ncsu.edu

23
DUKE-UNC BRAIN IMAGING ANALYSIS CENTER (BIAC)
  • fMRI Neuroscience acquisition and analyses
  • http//www.biac.duke.edu/

24
POSSIBLE APPLICATIONS(from NIH proposal T. Van
Dyke B. Tsui)
  • High-resolution pinhole SPECT studies
  • Can do a series of images with a single injection
    depending upon the length of the half-life of the
    radionucleotide.
  • Locating tumors and bone metastases
  • microPET studies
  • Locating a large variety of primary tumors and
    their metastases

25
POSSIBLE APPLICATIONS (from NIH proposal T.
Van Dyke B. Tsui)
  • MRI and MRS studies
  • Tumor growth
  • Changes in tumor vascularity
  • Optical Imaging studies
  • Luciferase localization
  • Reporter gene expression
  • MicroCT studies
  • Tumor growth

26
FUTURE DEVELOPMENTS(from NIH proposal T. Van
Dyke B. Tsui)
  • Use Animal Model systems for refining specific
    imaging technologiesi.e. technologies to measure
    blood flow and microvascular permeability within
    tumors.
  • Imaging results compared to histologic analyses
    can help develop technologies to measure disease
    progression and then be used to screen
    development of disease in backgrounds with
    additional genetic alterations.
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