Free Radicals, Antioxidants and Revolutionary Noninvasive Antioxidants Testing

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Free Radicals, Antioxidants and Revolutionary
Noninvasive Antioxidants Testing
The Road To Wellness Welcome To Your First Step
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American Journal of Health Promotion
November/December, 2002
66 Incompletely healthy
19 of those surveyed were completely healthy
with high levels of both physical and mental
health and a low level of illness.
18.8 completely unhealthy, defined as having low
levels of health with high levels of illness.
Two-thirds of the adults reported some degree of
mental or physical illness that kept them from
being completely healthy. Incompletely healthy.
OPTIMAL
DEAD
HEALTH continuum
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More money doesnt equal better health.

• The US came in 17th on the list of healthiest
  nations, Belgium was 1st.
• The US spends 13.1 of its GDP -- or 4,180
  annually per capita on health, compared to
  Belgium's 8.9 (2,172)
• Retail pharmacies filled 3 billion prescriptions
  in 2000.
• Reuters London March 25, 2002

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WHO survey 2000U.S.A. Today, associated press,
June 21, 2000,
On June 21, 2000, an article released by the
associated press reported that The United States
spends more per person on health care than any
other country, yet in overall quality its care
ranks 37th in the world.
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Annual Physical and Economic Cost of Medical
InterventionBy Gary Null PhD, Carolyn Dean MD
ND, Martin Feldman MD, Debora Rasio MD, Dorothy
Smith PhD
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We could have an even higher death rate by using
Dr. Lucien Leape's 1997 medical and drug error
rate of 3 million14. Multiplied by the fatality
rate of 14 percent (that Leape used in 1994 we
arrive at an annual death rate of 420,000 for
drug errors and medical errors combined16. If we
put this number in place of Lazorou's 106,000
drug errors and the Institute of Medicine's (IOM)
98,000 medical errors, we could add another
216,000 deaths making a total of 999,936 deaths
annually.
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8 of the top 10 causes of death are directly
related to poor nutrition.
1. Heart Disease 36.4 2. Cancer
22.3 3. CV Disease 7.1 4.
Accidents 4.6 5. Pulmonary Disease
3.6 6. Pneumonia, Flu 3.3 7. Diabetes
1.8 8. Suicide 1.5 9. Liver
Disease 1.2 10. Atherosclerosis 1.1 11.
All other causes 3.4
Dr. C. Everett Koop, Former Surgeon General
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National Cancer Institute
In a survey of 21,500 people, not a single
person consumed 100 of RDA for the 10 most
important nutrients!
Did you eat 6-10 Servings of Fruits and
Vegetables Today?
as recommended by the National Cancer Institute
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On a Typical Day

• 40 of Americans dont eat any fruit
• 20 of Americans dont eat any vegetables.
• 51 of Americans dont any vegetables other than
  potato or salad.

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Theories of Aging

• Dr. Rubner 1908 The Rate of Living Theory
• Linked metabolic rate and aging.
• Dr. Denham Harman 1954 The Free Radical Theory
  of Aging
• Dr. Johan Bjorksten 1968 The Crosslinkage Theory
  of Aging
• Dr. A. Cerami 1985 The Glycation Theory of Aging

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The Free Radical Theory of Aging

• In 1954 Dr. Denham Harman first described the
  theory of free radical aging.
• a single common process, modifiable by genetic
  and environmental factors, was responsible for
  aging and death of all living things.

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Aging Symptoms
Youre not getting older, just your
DNA (Headline from an article in Popular
Science, August 2002, p.44)
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The Bad Guys Free Radicals

• Superoxide (02-) A normal product of oxygen
  metabolism. Cell membranes, mitochondria and
  chromosomes are favorite targets.
• Hydrogen Peroxide (H2O2) One of the by-products
  of scavenging superoxide. A key culprit in lipid
  peroxidation which is linked to many diseases and
  early aging.
• Hydroxyl Radical (HO) Formed from incomplete
  processing of (H2O2). It is the most toxic of
  the free radicals.
• Singlet Oxygen (1O2) Involved in diseases of the
  joints (arthritis) but is most damaging to the
  eye.

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Free Radical Damage

• Mitochondria
• Free radical damage here alters the energy
  production of the cell, decreasing its ability
  to function. As the cells go so do we, less
  energy.

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Free Radical Damage

• The Cell Wall
• The membrane of the cell allows nutrients in
  and waste products out. When damaged by free
  radicals it can rupture, leak or become clogged
  resulting in premature death of the cell.

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Inflammation

• The second leading cause of free radical
  production in the body is caused by inflammation.
• If there are not enough antioxidants to
  counteract the inflammation the result is a
  chronic inflammatory disease. A great example is
  arthritis.

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If the cell membranes contain a higher
concentration of arachidonic acid, then pro
inflammatory eicosanoids will outnumber anti
inflammatory eicosanoids. This leads to chronic
inflammation.
Arachidonic Acid
Omega 6
Omega 3
Trans fat
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Free Radical Damage

• Peroxisome
• As a byproduct of the degradation of fatty
  acids (triglycerides) hydrogen peroxide is
  produced which is normally degraded by catalase
  however, under certain conditions some may escape
  into the cell causing damage.

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Free Radical Damage

• Lysosomes
• These little packets of enzymes which are
  designed to eat through anything except the
  membrane that contains them can be ruptured by
  free radicals and eat through the cell and the
  one next to it producing more free radicals as
  they go.

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Free Radical Damage

• Blood and tissue Lipids
• Free radicals cause lipid peroxidation.
  LDL cholesterol when damaged becomes sticky and
  can clog arteries and cause macrophages to become
  foam cells. This causes arteriosclerosis a
  leading cause in heart disease and stroke.

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Cholesterol

• Free radical damaged LDL Cholesterol is called
  lipoprotein A. Macrophages ingest the LpA and
  become foam cells. These burst and cause the
  characteristic yellow streak in arthrosclerosis.
• LDL Cholesterol does not appear to be a problem
  unless it oxidizes.

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From The American Journal of Clinical Nutrition
1991

• The amount of vitamin E in the blood stream
  predicted the risk of heart disease with 62
  certainty cholesterols predictive value was only
  28. When vitamin E and cholesterol were used
  together the predictive value rose to 83.
  Beta-carotene, vitamin C and E gave an 88
  predictability. Adding cholesterol to this made
  no change!

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Free Radical Damage

• The DNA
• Free radicals in the nucleus can attack a
  gene and damage the chemical bonds and alter the
  information transcribed or they can cause
  cross-linking not allowing it to replicate at all.

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Drugs That Reduce Antioxidant Status

• Aspirin
• Antacids
• Cholestyramines
• Statins
• Blood thinners
• Broad-spectrum antibiotics
• Anticonvulsanst
• Urinary antibiotics
• Corticosteroids

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Free Radicals Play a Major Role in the Following
Diseases

• Premature aging
• Cancers
• Heart disease
• Autoimmune diseases
• Rheumatoid arthritis
• Amyloid diseases (Alzheimer's)
• Radiation injury
• Cataracts
• Porphyria
• Retinopathy
• Parkinsons disease

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The Good Guys Natural Mechanisms to Control Free
Radicals

• Antioxidant Enzymes
• Catalase
• Glutathione peroxidase
• Glutathione reductase
• Superoxide dismutase (both Mn and Cu-Zn enzymes)
• Free Radical scavengers/chain-breakers
• Vitamins A, C, E, Coenzyme Q10, carotenoids
  (lycopene, beta-carotene, etc.)
• Flavonoids (querdetin, rutin, catechin, etc.)
• Uric acid, bilirubin, thiols (-SH)

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Important Antioxidant Catalysts

• Superoxide Dismutase
• 2O2- 2H ? O2 H2O2
• SOD requires Copper/Zinc or Manganese
• Glutathione Peroxidase and Catalase
• 2GSH H2O2 ? 2H2O O2
• 2 H2O2 ? 2H2O O2
• Selenium is required

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Vitamin E

• Vitamin E is perhaps the bodys most important
  fat-soluble antioxidant protecting cell membranes
  and lipids from oxidative free radical damage.
• The dl-alpha tocopherol form of vitamin E which
  is synthetic does not have the antioxidant
  potency of the natural form.
• Vitamin E has 8 forms alpha, beta, gamma and
  delta plus 4 tocotrienols.

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Vitamin C

• Vitamin C is a water soluble antioxidant which is
  also required in tissue growth and repair,
  adrenal gland function, production of anti-stress
  hormones, production of interferon and is needed
  for the metabolism of folic acid, tyrosine and
  phenylalanine.
• Vitamin C also regenerates vitamin E.

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Alpha-Lipoic Acid

• Has the unique ability to neutralize both fat and
  water soluble free radicals and is the key
  antioxidant in mitochondrial function.
• Promotes the bodys production and regeneration
  of glutathione and coenzyme Q10.
• Regenerates both vitamin E and C.
• Crosses the blood-brain barrier to protect the
  nerve system.

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Bioflavinoids

• Must be obtained through the diet and therefore
  are sometimes called vitamin P
• Quercetin found in onions, broccoli, and tomatoes
  and catechin found in green tea and grapes are
  powerful antioxidants and have been linked to
  cancer prevention.

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Carotenoids

• One of the most important antioxidant protectors
  against lipid peroxidation.
• Unlike the antioxidant vitamins carotenoids with
  a single molecule can scavenge up to 20 free
  radicals before it is destroyed.
• They are also very protective against singlet
  oxygen radicals.

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Indisputable
3 Decades 30,000 Published Papers Document the
link between Free Radical Activity and Cell
Damage
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180 Degrees
June 19, 2002
We recommend that ALL ADULTS take a
multi-vitamin daily
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• The amount of key antioxidants that many
  different species maintain in their body is
  directly proportional to their lifespan."Richard
  Cutler, M.D., Director of Anti-aging Research at
  the National Institutes of Health

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The Future is NOW!
April 6, 1992
On Antioxidants vs. Free Radicals
Antioxidants may one day revolutionize health
care. Screening people through a simple...test
to assess how much damage free radicals have done
to tissue, much as patients today are screened
for high cholesterol.
Biochemist, William Pryor, director of
the Biodynamics Institute at Louisiana State
University
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The Biophotonic Scanner
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Raman ScatteringSir C. V. Raman, Nobel Prize in
Physics 1930
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Raman Spectroscopy of Carotenoids
Carotenoids have a characteristic Raman spectral
fingerprint generated from vibrations of their
long carbon backbone. Any other molecule would
produce a different fingerprint (peaks with
different locations and intensities)
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Lycopene and ? -Carotene

• Most potent singlet oxygen quenchers found in the
  human body
• Predominant carotenoids found in skin
• Lipophilic molecules making them especially well
  suited to act as chain-breaking antioxidants in
  the lipid-rich epidermis
• Resonant Raman spectroscopy for non-invasive
  detection ?

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What Do We Measure?

• Carotenoid antioxidants in stratum corneum

Carotenoids protect skin cells (DNA) from
harmful UV Light
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Site of Action
Blood and urine tests can be altered by a few
meals.
It takes 4-6 weeks or longer for antioxidants to
reach skin cells.
Only Pharmanex can measure at the Site of
Action.
Cell Body Site of Action!
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Think Range
50,000 and Higher
40,000 to 50,000
30,000 to 40,000
20,000 to 30,000
10,000 to 20,000
Less than 10,000
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The Antioxidant Miracle
Lester Packer Pharmanex Scientific Advisory Board
Member Founder of Packer Labs at Berkeley 70
Books Published 700 Articles THE FATHER OF THE
ANTIOXIDANT THEORY
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Everyone
Everyone should use the Pharmanex
Bio-Photonic Scanner to learn their optimal Body
Defense Score. Once you have your number, work
to increase it and maintain it with a diet rich
in fruits, vegetables and vitamin
supplements Lester Packer, PhD. UC Berkeley As
Quoted on CNN/Money.com
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Carotenoids as a Biomarker for Overall
Antioxidant Status

• Studies show that carotenoids are an excellent
  biomarker for overall antioxidant status.
• Carotenoids are sacrificial antioxidants when
  they are depleted it puts a great deal of stress
  on the other antioxidant mechanisms.
• A study presented at the American College of
  Nutrition showed that the variability of the
  biophotonic method was significantly less (9.48
  percent) than that of the serum/HPLC method. The
  correlation between the two was also high
• (r 0.82).

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LifePak 40 Antioxidants

• All 8 forms of natural vitamin E 300 IU
• Buffered vitamin C 500 mg
• Selenium, Copper, Zinc, Manganese
• 6 Carotenoids
• as found in 5-10 fruits vegetables per day
• Over 20 Flavonoids
• 6 Catechins, Quercetin, naringenin, hesperidin,
  3 Soy isoflavones, Over 10 grape seed
  polyphenols
• Alpha-Lipoic Acid 30-50 mg

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SupplementWatch.com

• SupplementWatch rates LifePak 1!

LifePak has been the subject of two clinical
studies designed to assess the antioxidant
benefits of the product quite a significant
fact when you consider that we know of NO other
supplement companies that have sponsored clinical
studies on their multivitamin products.
We applaud Pharmanex for their efforts in this
regard.
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Brilliant Minds Endorse...

• Lester Packer Antioxidant Miracle. Father of
  antioxidants. This validates my lifes work.
• James Rippe, MD Tufts, Boston (Cardiologist)
• James Balch, MD author The Prescription for
  Nutritional Healing- 8 million copies sold
• Koji Nakanishi Columbia. Discovered Ginkgo
  Biloba
• Carl Dierassi, Stanford birth control pill.
• Chi Huey Wong Scripps Institute, appointed to
  the National Academy of Sciences

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Sick vs Wellness Industries

• The sickness industry was 1.8 trillion last
  year. It is 1/7 of our economy and is the
  largest segment.
• The fastest growing market is in health and
  wellness which was 300 billion last year and is
  projected to rise to 1 trillion by the year
  2010.
• Baby boomers are 25 of the population and
  represent 50 of the spending.

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American Journal of Health Promotion
November/December, 2002
66 Incompletely healthy
19 of those surveyed were completely healthy
with high levels of both physical and mental
health and a low level of illness.
18.8 completely unhealthy, defined as having low
levels of health with high levels of illness.
Two-thirds of the adults reported some degree of
mental or physical illness that kept them from
being completely healthy. Incompletely healthy.
OPTIMAL
DEAD
HEALTH continuum
52
8 of the top 10 causes of death are directly
related to poor nutrition.
1. Heart Disease 36.4 2. Cancer
22.3 3. CV Disease 7.1 4.
Accidents 4.6 5. Pulmonary Disease
3.6 6. Pneumonia, Flu 3.3 7. Diabetes
1.8 8. Suicide 1.5 9. Liver
Disease 1.2 10. Atherosclerosis 1.1 11.
All other causes 3.4
Dr. C. Everett Koop, Former Surgeon General
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Health

• Is a process that is acquired through
• action
• that buys you

TIME
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You may have less time than you think!!!!!