Susan%20Hollingsworth

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Bacteriology Lab

• Susan Hollingsworth
• Aaron Kaiser
• Biology 110
• Lab smh092404

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Purpose

• To observe multiple environments that harbor
  bacterial growth and determine which environment
  under investigation is most conducive to
  bacterial growth
• Environments under investigation
• air
• soil
• washed/unwashed hands
• door handle of mens bathroom
• raw chicken
• pond water

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Hypothesis

• Pond Water
• largest and most diverse population of bacteria
  due to a vast array of living and decomposing
  organisms that are vulnerable to bacterial
  colonization
• aquatic medium that receives sunlight (some
  bacteria are photosynthetic)
• exposed to air which transports bacterial spores.

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Materials

• 7 sterile Petri dishes with nutrient agar
• Wax pencil
• 9 bacterial inoculating loops
• Sterile cotton swab
• Piece of raw chicken
• Soil sample
• Pond water sample
• Hand soap
• Sterile water
• Parafilm strips
• Light microscope
• Dissecting microscope
• incubator

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Method

• Label each Petri dish
• Air sample
• Use Streak Plate method to apply sample

(b)
(c)
(a)

• Figure 1. Isolating bacterial colonies using the
  Streak Plate method.

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Data Interpretations

• Unwashed/Washed hand sample
• Recover dish labeled air.
• Wrap cultures with Parafilm and incubate upside
  down for 1 week.
• The Petri dishes will be analyzed by using colony
  morphology characteristics to see which one has
  the most species of bacteria. This will be
  accomplished by identifying and tabulating the
  number of different colonies present in each
  sample.

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Table 1. Bacteria colony morphology Air
Colony Size Shape Margin Surface Color
a. 6mm Irregular Lobate Wrinkled Milky white
b. 4mm Round Wavy Smooth White center, clear surrounding
c. 13mm Irregular Lobate Smooth White center, milky white surrounding
d. 5mm Irregular Wavy Smooth Yellow, gold, clear surrounding
e. 9.5mm Irregular Wavy Smooth, contoured edges Tan center, white ring, clear ring
Fig. 2 Bacteria present in Air environemnt
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Table 2. Bacteria colony morphology Chicken
Colony Size Shape Margin Surface Color
a. 1mm Round Lobate Wrinkled Creamy white to slightly yellow
b. 5mm Round Smooth Smooth white
Fig 3. Bacteria found on Chicken environment
Table 3. Bacteria colony morphology Soil
Colony Size Shape Margin Surface Color
a. 10mm No defined shape No defined margin Wrinkled Clear
b. Less than 1mm Irregular Lobate Wrinkled Cream white
Fig 4. Bacteria found on soil environment
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Table 5. Bacteria colony morphology washed hand
Colony Shape Size Margin Surface Color
a. 3mm Round Lobate Smooth Creamy white
b. 4mm Round Lobate Smooth Clear
c. Punctiform Round Smooth Smooth white
Fig 5. Bacteria found on washed and unwashed hand
environments
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Table 4. Bacteria colony morphology unwashed
hand
Colony Size Shape Margin Surface Color
a. 8mm Irregular Smooth Smooth Yellow
b. 8mm Round Lobate Smooth Clear to creamy white
c. 3mm Round Smooth Concentric White
d. 2mm Round Lobate Smooth Clear to off white
e. 3mm Round Lobate Wrinkled and smooth Clear
f. Punctiform on e. Round Smooth Smooth Slightly white to clear
g. 7mm Irregular Wavy Smooth Creamy white
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Table 6. Bacteria colony morphology pond water
Colony Size Shape Margin Surface Color
a. 4mm Round Lobate Smooth Tan to white
b. 1mm Round Smooth Smooth Orange
c. 5mm Round Smooth Smooth Clear to off white
d. 30mm Irregular Lobate Smooth trasparent
Fig 7. Bacteria found on pond water environment
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Table 7. Bacteria colony morphology Mens
bathroom door
Colony Size Shape Margin Surface Color
a. 3mm Round Lobate Smooth Clear
b. 3mm Round Lobate Smooth Orange
c. 10mm Round Smooth Smooth yellow
d. 10mm Round Smooth Smooth white
e. 6mm Round Wavy Mucoidal white
Fig 8. Bacteria found on bathroom door environment
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Side Study Effect of Antibiotics on E. coli
growth
Antibiotic Sensitivity
Chloramphenicol-30mg NS
Penicillin-10mg NS
Nalidixic Acid-30mg VS
Norobiocin-30mg NS
Streptomycin-10mg VS
Erythromycin-15mg S
Tetracycline-30mg VS
Kanamycin-30mg VS
Fig 9. E coli growth among antibiotics