How to make a poster presentation

1

• How to make a poster presentation
• Dr Nick Deacon
• Director (Research), Monash Gippsland

2
www.gippsland.monash.edu.au/research
3
www.gippsland.monash.edu.au/research/posterprepar
ation
4
Poster Title (28pt)Authors (19pt)Addresses
(14pt)
Sub Heading appears in Arial 12pt size, bold,
left aligned Body text appears in Arial, 9pt
size, left aligned.
Sub Heading appears in Arial 12pt size, bold,
left aligned Body text appears in Arial, 9pt
size, left aligned.
Sub Heading appears in Arial 12pt size, bold,
left aligned Body text appears in Arial, 9pt
size, left aligned.
Sub Heading appears in Arial 12pt size, bold,
left aligned Body text appears in Arial, 9pt
size, left aligned.
Sub Heading appears in Arial 12pt size, bold,
left aligned Body text appears in Arial, 9pt
size, left aligned.
Add other logos here
5
Regulation of expression of the HIV-1vpo gene
AuthorsAddresses
Sub Heading appears in Arial 12pt size, bold,
left aligned Body text appears in Arial, 9pt
size, left aligned.
Sub Heading appears in Arial 12pt size, bold,
left aligned Body text appears in Arial, 9pt
size, left aligned.
Sub Heading appears in Arial 12pt size, bold,
left aligned Body text appears in Arial, 9pt
size, left aligned.
Sub Heading appears in Arial 12pt size, bold,
left aligned Body text appears in Arial, 9pt
size, left aligned.
Sub Heading appears in Arial 12pt size, bold,
left aligned Body text appears in Arial, 9pt
size, left aligned.
Add other logos here
6
Regulation of expression of the HIV-1vpo gene
Soo Huey Yap (supervisor Nick Deacon)Addresses
Sub Heading appears in Arial 12pt size, bold,
left aligned Body text appears in Arial, 9pt
size, left aligned.
Sub Heading appears in Arial 12pt size, bold,
left aligned Body text appears in Arial, 9pt
size, left aligned.
Sub Heading appears in Arial 12pt size, bold,
left aligned Body text appears in Arial, 9pt
size, left aligned.
Sub Heading appears in Arial 12pt size, bold,
left aligned Body text appears in Arial, 9pt
size, left aligned.
Sub Heading appears in Arial 12pt size, bold,
left aligned Body text appears in Arial, 9pt
size, left aligned.
Add other logos here
7
Regulation of expression of the HIV-1vpo gene
Soo Huey Yap (supervisor Nick Deacon)School of
Applied Sciences and Engineering
Sub Heading appears in Arial 20pt size, bold,
left aligned Body text appears in Arial, 16pt
size, left aligned.
Sub Heading appears in Arial 20pt size, bold,
left aligned Body text appears in Arial, 16pt
size, left aligned.
Sub Heading appears in Arial 20pt size, bold,
left aligned Body text appears in Arial, 16pt
size, left aligned.
Sub Heading appears in Arial 20pt size, bold,
left aligned Body text appears in Arial, 16pt
size, left aligned.
Sub Heading appears in Arial 20pt size, bold,
left aligned Body text appears in Arial, 16pt
size, left aligned.
Add other logos here
8
sORF Regulation HIV-1vpo Gene Expression.Soo
Huey Yap (supervisor Nick Deacon)School of
Applied Sciences and Engineering
Introduction As well as the nine structural,
regulatory and auxiliary genes the HIV-1 genome
also encodes, in the anti-sense orientation, a
highly hydrophobic protein we have termed Vpo
(viral protein encoded in the opposite
orientation). The function of the protein is
unknown but it has been observed in association
with infected cell membranes and virus
particles. The genes coding region is preceded
by a series of 6 short open reading frames. The
aim of this work is to determine the potential
role of the sORFs in regulation of Vpo expression.
Sub Heading appears in Arial 12pt size, bold,
left aligned Body text appears in Arial, 9pt
size, left aligned.
Sub Heading appears in Arial 12pt size, bold,
left aligned Body text appears in Arial, 9pt
size, left aligned.
Sub Heading appears in Arial 12pt size, bold,
left aligned Body text appears in Arial, 9pt
size, left aligned.
Sub Heading appears in Arial 12pt size, bold,
left aligned Body text appears in Arial, 9pt
size, left aligned.
Add other logos here
9
sORF Regulation HIV-1vpo Gene Expression.Soo
Huey Yap (supervisor Nick Deacon)School of
Applied Sciences and Engineering
Introduction As well as the nine structural,
regulatory and auxiliary genes the HIV-1 genome
also encodes, in the anti-sense orientation, a
highly hydrophobic protein we have termed Vpo
(viral protein encoded in the opposite
orientation). The function of the protein is
unknown but it has been observed in association
with infected cell membranes and virus
particles. The genes coding region is preceded
by a series of 6 short open reading frames. The
aim of this work is to determine the potential
role of the sORFs in regulation of Vpo expression.
Sub Heading appears in Arial 12pt size, bold,
left aligned Body text appears in Arial, 9pt
size, left aligned.
3LTR
V IV III II I
U3
U5
R
vpo
(189, 36, 48, 28, 15, 6aa)
NS transcription
I-V
II-V
III-V
IV-V
V
Figure 1. Segments amplified by PCR from
HIV-1NL43 to prepare plasmid constructs
containing the depicted vpo upstream sORFs.
Sub Heading appears in Arial 12pt size, bold,
left aligned Body text appears in Arial, 9pt
size, left aligned.
Sub Heading appears in Arial 12pt size, bold,
left aligned Body text appears in Arial, 9pt
size, left aligned.
Materials and Methods HEK293 cells (5x106) were
transfected with 5 ?g DNA of the various sORF
construct plasmids (Fig.1.), pEGFP and pENI
controls. The level of green fluoresence
observed in lysates of cells harvested after
48hrs incubation was determined for each
construct .
Add other logos here
10
sORF Regulation HIV-1vpo Gene Expression.Soo
Huey Yap (supervisor Nick Deacon)School of
Applied Sciences and Engineering
Introduction As well as the nine structural,
regulatory and auxiliary genes the HIV-1 genome
also encodes, in the anti-sense orientation, a
highly hydrophobic protein we have termed Vpo
(viral protein encoded in the opposite
orientation). The function of the protein is
unknown but it has been observed in association
with infected cell membranes and virus
particles. The genes coding region is preceded
by a series of 6 short open reading frames. The
aim of this work is to determine the potential
role of the sORFs in regulation of Vpo expression.
Results Cells transfected with parental plasmid
pEGFPN1 showed gt1000 fluorescent units per ?g
total protein compared with EGFP- of green
fluorescence indicating high level expression of
the EGFP gene compared to the pENI EGFP- specific
fluorescence (Fig. 2a). Insertion of the vpo
upstream sORFs led to gt90 inhibition of EGFP
reporter gene expression in constructs I-V to
IV-V, however, construct ORF-V induced approx 50
inhibition.
3LTR
V IV III II I
U3
U5
R
vpo
(189, 36, 48, 28, 15, 6aa)
NS transcription
sORF I-V
sORF II-V
sORF III-V
sORF IV-V
sORF V
Figure 1. Segments amplified by PCR from
HIV-1NL43 to prepare plasmid constructs
containing the depicted vpo upstream sORFs.
Materials and Methods HEK293 cells (5x106) were
transfected with 5 ?g DNA of the various plasmid
constructs of pEGFPN1 containing the sORF
fragments upstream of the EGFPN1 ORF (Fig.1.),
parental plasmid pEGFPN1 and EGFP- plasmid pENI
as controls. The level of green fluoresence
observed in lysates of cells harvested after
48hrs incubation was determined for each
construct .
Sub Heading appears in Arial 12pt size, bold,
left aligned Body text appears in Arial, 9pt
size, left aligned.
Figure 2. Effect of vpo sORF constructs on EGFP
reporter gene expression expressed as (A)
specific fluorescence and (B) inhibition of
expression.
Add other logos here
11
sORF Regulation HIV-1vpo Gene Expression.Soo
Huey Yap (supervisor Nick Deacon)School of
Applied Sciences and Engineering and Macfarlane
Burnet Institute, respectively.
Introduction As well as the nine structural,
regulatory and auxiliary genes the HIV-1 genome
also encodes, in the anti-sense orientation, a
highly hydrophobic protein we have termed Vpo
(viral protein encoded in the opposite
orientation). The function of the protein is
unknown but it has been observed in association
with infected cell membranes and virus
particles. The genes coding region is preceded
by a series of 6 short open reading frames. The
aim of this work is to determine the potential
role of the sORFs in regulation of Vpo expression.
Results Cells transfected with parental plasmid
pEGFPN1 showed gt1000 fluorescent units per ?g
total protein compared with EGFP- of green
fluorescence indicating high level expression of
the EGFP gene compared to the pENI EGFP- specific
fluorescence (Fig. 2A). Insertion of the vpo
upstream sORFs led to significant inhibition
(Fig. 2B) of the EGFP reporter gene. Constructs
containing sORFs I-V to IV-V led to gt90
inhibition of gene expression. Construct ORF-V,
however, led to only approx 50 inhibition.
3LTR
V IV III II I
U3
U5
R
vpo
(189, 36, 48, 28, 15, 6aa)
NS transcription
sORF I-V
sORF II-V
sORF III-V
sORF IV-V
sORF V
Figure 1. Segments amplified by PCR from
HIV-1NL43 to prepare plasmid constructs
containing the depicted vpo upstream sORFs.
A
B
Materials and Methods HEK293 cells (5x106) were
transfected with 5 ?g DNA of the various plasmid
constructs of pEGFPN1 containing the sORF
fragments upstream of the EGFPN1 ORF (Fig.1.),
parental plasmid pEGFPN1 and EGFP- plasmid pENI
as controls. The level of green fluoresence
observed in lysates of cells harvested after
48hrs incubation was determined for each
construct .
Discussion The vpo sORFs have a regulatory
effect on expression of the downstream gene. All
vpo sORFreporter gene constructs significantly
down-regulated gene expression suggesting that in
HIV-1 vpo gene expression may be regulated at the
translational level by the presence of upstream
short open reading frames.
Figure 2. Effect of vpo sORF constructs on EGFP
reporter gene expression expressed as (A)
specific fluorescence and (B) inhibition of
expression.
Add other logos here
12
sORF Regulation HIV-1vpo Gene Expression.Soo
Huey Yap (supervisor Nick Deacon)School of
Applied Sciences and Engineering
Introduction As well as the nine structural,
regulatory and auxiliary genes the HIV-1 genome
also encodes, in the anti-sense orientation, a
highly hydrophobic protein we have termed Vpo
(viral protein encoded in the opposite
orientation). The function of the protein is
unknown but it has been observed in association
with infected cell membranes and virus particles.
The genes coding region is preceded by a series
of 6 short open reading frames. The Aim of this
work is to determine the potential role of the
upstream sORFs in regulation of Vpo
expression. To determine the role of upstream
sORFs in the regulation of gene expression HEK293
cells (5x106) were transfected with 5?g DNA of
the various plasmid constructs of pEGFPN1
containing the sORF fragments upstream of the
EGFPN1 gene in the parental plasmid pEGFPN1 and
EGFP- plasmid pENI as controls. The level of
green fluoresence observed in lysates of cells
harvested after 48hrs incubation was determined
for each construct . Cells transfected with
parental plasmid pEGFPN1 showed gt1000 fluorescent
units per ?g total protein compared with EGFP- of
green fluorescence indicating high level
expression of the EGFP gene compared to the pENI
EGFP- specific fluorescence. Insertion of the vpo
upstream sORFs led to significant inhibition of
the EGFP reporter gene. Constructs containing
sORFs I-V to IV-V led to gt90 inhibition of gene
expression. Construct ORF-V, however, led to
only approx 50 inhibition. The vpo sORFs have a
regulatory effect on expression of the downstream
gene. All vpo sORFreporter gene constructs
significantly down-regulated gene expression
suggesting that in HIV-1 vpo gene expression may
be regulated at the translational level by the
presence of upstream short open reading frames.
Add other logos here