Welcome to Bionanotechnology Chem 140240

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Welcome to BionanotechnologyChem 140/240
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Instructor
Prof. Heather D. Maynard Tel. (310)
267-5162 Office 4505B Molecular Sciences
Building E-mail maynard_at_chem.ucla.edu Office
hours Tuesdays 100-200 pm by
request Virtual office hours available at
voh.chem.ucla.edu
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Location and Time
4216 Young Hall 800-935 am with a 5 minute
break in the middle
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Teaching Assistant
Chris Kolodziej Office 3114 Molecular Sciences
Building E-mail kolodzic_at_chem.ucla.edu Office
hours Mondays, 100-300 pm
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Course Motivation
In January 2000, President Clinton announced a
national initiative focusing on nanotechnology.
Since then, the impact on academia, government
laboratories, and industry has been significant.
Likewise, the impact on manufacturing and
products is expected to be significant.
According to the National Nanotechnology
Initiative (NNI), The worldwide workforce
necessary to support the field of nanotechnology
is estimated at 2 million by 2015.(1) This
necessitates training of undergraduates and
graduates in this multidisciplinary field of
research. A quote by Mihail C. Roco, NSF Senior
Advisor for Nanotechnology and NSET Chairman,
nicely summarizes the need for nanotechnology
training A five-year goal of the NNI is to
ensurestudent access to education in nanoscale
science and engineering is enabled in at least
25 of the research universities.(1)
(1) http//www.nano.gov/html/edu/home_edu.html
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Support from UCLA
UCLA is one of the leading institutions in the
nation in nanotechnology. Jointly with UC Santa
Barbara, the California NanoSystems Institute
(CNSI) is focusing on research and education in
the nano areas. In addition, UCLA has a history
of interdisciplinary education and hosts, for
example, the NSF-sponsored Center for Scalable
and Integrated NanoManufacturing (SINAM). One of
the goals of both of these entities is to educate
students broadly in nanotechnology. In addition,
Organic Chemistry Division has recently created a
Nano Materials Course thrust. This course is
needed as an elective for this course.
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Course Objectives
The objective of this course is to teach the
basic principles behind the fabrication,
characterization, and utilization of
biologically-derived materials ordered at the
nanoscale.
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General Topics

• a) Basic chemical, physical, and biological
  principles of nanotechnology
• Fabrication and synthesis of ordered
  nanostructured biomaterials
• c) Characterization and application of the
  bionanomaterials.

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Specific Topics
- Definitions and basic principles of
nanotechnology and bionanotechnology -
Nanografting techniques and conductive atomic
force microscopy - Dip-pen nanolithography
(indirect and direct) and nanopen - Nanocontact
printing and nanoimprint lithography -
Photolithographic approaches and electron beam
lithography - Protein (artificial and natural)
design and assembly - Polymer and small molecule
self assembly and templating - Biological
templating and other bottoms-up approaches -
Protein-polymer conjugates for therapeutic
applications - Polymers and inorganic particles
for drug delivery, gene delivery, and imaging -
Characterization by microscopy, electrochemistry,
and surface plasmon resonance - Applications in
the areas of disease detection, diagnostics, and
biomaterial coatings
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Calendar
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Requirements - Reading
There is no textbook for this course. Reading
material consists of journal articles. Reading
assignments will be provided as a handout in
class and will also be posted on VOH,
announcements section. Journal articles need
to be downloaded from the UCLA library website.
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Requirements - Reading
Reading of assigned journal articles prior to
class is required. Discussion of this material
during class is expected from all class
participants. Copies of helpful reviews will be
provided in class as needed.
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Requirements - Exams
Midterm Tuesday, February 5, 2008 (800 950
AM) Final Exam Tuesday, March 11, 2008 (800
950 AM) (Note Exam is in class)
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Requirements - Exams
Exams will cover material presented in lecture.
The midterm covers material from January 8-31,
2008. The final exam covers material from
February 7-March 6, 2008.
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Requirements - Exams
Unexcused absences will count as zero. The use
of any unapproved electronic device (cell phone,
palm pilot, etc.) during the exam is forbidden.
Anyone who uses such a device will receive a zero
for that exam. Unclaimed exams will be available
from the TA.
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Grading Chem 140

• Participation in class discussions 30
• Midterm examination 35
• - Final examination 35

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Grading Chem 240

• Participation in class discussions 25
• Midterm examination 30
• Final examination 30
• - Report (Due on March 13, 2008) 15

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Final Report Chem 240
Choose 1 paper form recent literature that was
not discussed in class. The paper should have
been published (or be available online) on or
after January 1, 2008. Write a 2 page, single
spaced critical review of the manuscript.
Hypothesis and goals of the study, experimental
results, and conclusions should be clearly
indicated in your report. Discussion of paper
selection with TA is encouraged
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Final Report Chem 240
Papers should be in the area of
bionanotechnology. Potential journals include
Nano Lett., Science, Nature, JACS, Angew.
Chem., Macromolecules, Biomacromolecules, Adv.
Mat., Langmuir, Nanotechnology, ACS Nano
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Other
The use of cell phones during class is disruptive
to others and will not be tolerated. Cell
phones should be turned off prior to the
beginning of class.
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Obtaining Journal Articles
Search UCLA library for journal. (Note You
should be logged on to the UC system in order to
have access from home.) http//eresources.librar
y.ucla.edu/sel/index.cfm
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Nano
A prefix meaning 10-9 or one billionth
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Nanotechnology
-A branch of engineering or science that deals
with things smaller than 100 nanometer -Fabricati
on technology in which objects are designed and
built by the specification and placement of atoms
or molecules or where at least one dimension is
on the scale of nanometers. -Collective term
referring to technological developments on the
nanometer scale (i.e. 0.1-100 nm)
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Bionanotechnology
Learn from nature. Modify and find technical
uses for natural nanocomponents that are
biologically derived. (example nanomoters of
ATP synthase)
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Nanobiotechnology
Branch of nanotechnology with biological and
biochemical applications or uses (example
nanosensors based on DNA and gold
nanoparticles)
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Introduction to AFM
Slide from Lía Pietrasanta
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AFM
http//stm2.nrl.navy.mil/how-afm/how-afm.html
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AFM Cantilevers
Etched Silicon
Silicon Nitride
10-40 nm
5-10 nm
Slide from Lía Pietrasanta
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AFM Tip
http//stm2.nrl.navy.mil/how-afm/how-afm.html
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Spatial Resolution Depends on Tip Dimensions
C. Bustamante
Slide from Lía Pietrasanta
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Mechanism of Image Formation
object tip geometry image
Spatial resolution in AFM is not well-defined
depends on tip and sample Tip convolution is not
linear results do not add up
Slide from Lía Pietrasanta
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Tip Artifacts
Tip Shape
Slide from Lía Pietrasanta
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Tip Artifacts
Slide from Lía Pietrasanta
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Imaging Modes
Slide from Lía Pietrasanta
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Interatomic force vs. distance curve
Slide from Lía Pietrasanta
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Taping Mode Elasticity
http//stm2.nrl.navy.mil/how-afm/how-afm.html
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Sample preparation
II-Prerequisites
Slide from Lía Pietrasanta
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Advantages of Using AFM to Study Biological
Samples

• High Resolution, 3-Dimensional Measurements
• Lateral nm Vertical sub-Angstrom
• Measurements Performed under Native Conditions
• In fluid (buffer, growth media, etc.) or gas
  (CO2, N2, etc.) environment
• At biologically relevant temperatures
• Sample Preparation
• No staining or coating required native surface
  remains unaltered

Slide from Lía Pietrasanta
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Limitations of AFM

• Sample Immobilization
• Covalent bonds, electrostatic forces
• Biological glues collagen, poly-L-lysine
• Glues double-sided tape, 5-minute epoxy
• Z Range
• Soft Samples, Hard Tips
• possible sample deformation
• Tip Contamination

Slide from Lía Pietrasanta
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MIMAFM
Digital Instruments-Veeco
Slide from Lía Pietrasanta