Kein Folientitel

1
Microtubules and polarized growth in Aspergillus
nidulans
Reinhard Fischer Max-Planck-Institute for
Terrestrial Microbiology
2
Polarised growth and the Spitzenkörper
B. Robertson, Athens GA
3
Conventional kinesin is required for hyphal
extension (Natalia Requena)
Wild type
DkinA
Requena et al., 2001, Mol. Mirobiol. 42 121-132
4
Polarised growth and the Spitzenkörper
Conventional Kinesin
B. Robertson, Athens GA
5
Cortical interactions of microtubules Nuclear
migration in S. cerevisiae
Num1 (ApsA)
Yamamoto et al., 2003, JCS 1164501-4512
6
Polarity mutants in S. pombe Dtea1 and Dtea2
Browning et al., 2000, JCB, 15115-27
7
Microtubule organization in Dtea1 and Dtea2
strains
Dtea1
Dtea2
WT
Browning et al., 2000, JCB, 15115-27
Mata and Nurse, 1997, Cell 89939-949
Tea1 destabilizes microtubules Tea2 stabilizes
microtubules
8
KipA deletion affects polarized growth (Sven
Konzack)
Wild type
kipA (tea2) mutant
9
KipA deletion affects Spitzenkörper position
10
KipA is required for microtuble organization in
the tip
Difference to S. pombe Dtea2
11
KipA localization
Sven Konzack
12
Microtubule dynamics Xin Xiang, Ron Morris
KipA is delivered in the tip
Morris, Curr. Opin. Cell Biol. 1554-59,
2003 Efimov, Mol. Biol. Cell 14871-888,
2003 Zhang et al., Mol. Biol. Cell 15 , 2003
13
Microtubule plus end
TeaA?
Hestermann et al., 2002
14
KipA and polarized growth
DkipA
Wild type
KipA
TeaA?
Conventional Kinesin
Putative TeaA receptor
15
DkipA and DteaA display distinct phenotypes
16
Microtubule plus end
Does KipA transport other cargoes?
Hestermann et al., 2002
17
Localization of AlpA in A. nidulans(Cathrin Enke)
Mitosis
GFP-AlpA
18
Deletion of alpA
19
Microtubule plus end
Curved hyphae KipA AlpA (XMAP) NudF
(LIS1) Cortical protein TeaA No curved
hyphae NudA (dynein) Cortical protein ApsA
Hestermann et al., 2002
20
Different cortical interactions for nuclear
migration and polarized growth
21
Microtubule polarity in A. nidulans
Microtubule tracks are used several times
22
Microtubule organization in A. nidulans
23
Tools Fluorescent proteins
Toews et al., 2004, Curr. Genet. online
24
High-throughput tagging (Gateway)
Gene of interest
Toews et al., 2004, Curr. Genet. online
25
Anne Blumenstein
Kay Vienken
Alex Schütz
Sven Konzack
Cathrin Enke
Daniel Veith
Nicole Scherr
Patricia Rischitor
fischerR_at_staff.uni-marburg.de