Title: International Hands-on workshop on genotoxiciy monitoring bioassays for environmental pollution
1International Hands-on workshop on genotoxiciy
monitoring bioassays for environmental pollution
- University of Silesia, Jagiellonska 28,
- 40-032 Katowice, Poland
- Directed by
- Prof. Jolanta Maluszynska, Department of Plant
Anatomy and Cytology, University of Silesia,
Katowice, Poland - Prof. Siegfried Knasmueller, Institute for Cancer
Research, The Medical University of Vienna,
Vienna, Austria - Prof. Michael Plewa, University of Illinois,
Champaign-Urbana, IL, USA
2International workshop on genotoxicity monitoring
bioassays for environmental pollution
Group photo of the participants
3Prof. Knasmueller (left) and Prof. Maluszynska
(right) discuss workshop plan
4Prof. Jolanta Maluszynska giving instructions at
the workshop, demonstrating Fluorescent in situ
hybridization (FISH) assay (photomicrograph was
not available)
5Instructor of the workshop giving a lecture to
the participants.
6Prof. Michael Plewa of the University of Illinois
demonstrating the slide preparation for Comet
test for clastogens
7Photomicrograph of a comet tail as the result of
DNA damage by clastogens
8Mr. Bernhard Majer of the medical University of
Vienna prepares Allium roots and Tradescantia
cuttings for chromosome aberration and
micronucleus assays .
9Tradescantia plants bearing young inflorescences
provide the test material for both Tradescantia-
Stamen-Hair (Trad-SHM) and Tradescantia-Micronucle
us (Trad-MCN) assays
10Photomicrograph of Sister-Chromatid-Exchange
(SCE) in Tradescantia root tip cell
Photomicrograph of Sister-Chromatid-Exchange
(SCE) in Tradescantia root tip cell cell.
11Dissection of flower buds in preparation of
Trad-MCN slides from the meiotic microsporocytes
12 Preparing stamen hairs of Tradescantia flower
under a dissecting microscope for scoring
frequencies of pink mutation events in the
Trad-SHM assay.
13MCN
MCN
Photomicrograph showing micronuclei (MCN) among
the resting stage root tip cells in the
subsequent generation of treated nuclei
14MCN
MCN
MCN
Photomicrograph of the tetrad stage of
microsporocyte of Tradescantia showing
micronuclei (MCN) among the synchronized tetrads
in Trad-MCN assay
15Scoring pink mutation events under the dissecting
microscope in the Tad-SHM assay
16Tradescantia flowers ready for scoring pink
mutation events after a 16- minute refrigeration
in the Trad-SHM assay
17blue
pink
blue
pink
Photomicrograph of orderly aligned stamen hairs
showing 3 pink mutation events in this field of
view in the Trad-SHM assay
18Collecting data for frequencies of mutation and
micronueli in treated and control groups of
Trad-SHM and Trad-MCN assays
19DATA analysis using computer software and print
out for report write up
20After a days hard work, participants took a tour
around town
21Relaxing and shopping in the Mall in the city of
Katowice, Poland
22Lets have some coffee and snack
23A walk outside to have some fresh air