The Keys to Laboratory Safety: Semester Review

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The Keys to Laboratory Safety Semester Review

• Bill Diesslin
• March 3, 2009

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Chemical Safety in the LabRisk Management
Name two federal agencies that have regulations
that deal with chemical use in the laboratory.
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Chemical Risk Management
What is the name of the document that is the
primary resource for information on how to use
chemicals in laboratories at ISU?
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ISU Laboratory Safety Manual

• Introduction
• Process Planning
• Emergency Planning
• Equipment
• Training
• General Lab Safety Practices
• Safety Practices for Specific Hazards
• Management of Unwanted Lab Materials (Waste)
• Exposure Assessment and Medical Care
• Appendix I Forms
• Appendix II Lab Related Manuals

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ISU Laboratory Safety Manual

• YOUR RESPONSIBILITIES
• Familiarize yourself with the document
• Be aware of lab hazards
• Follow Standard Operating Procedures
• Review processes with major professor
• Use equipment properly (i.e., PPE, fume hoods,
  etc.)
• Label, store dispose of chemicals properly
• Manage chemical inventories
• Be a good example to others

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Personal Protective Equipment
Chemical-resistant gloves are commonly used in
the laboratory. Name 3 things to be aware of
when selecting and using chemical-resistant
gloves.
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Personal Protective Equipment

• PPE is last line of defense
• Identify the hazards
• Selecting the right PPE
• Types available
• Minimum required in a lab
• Special cases
• Your responsibilities when wearing PPE
• Regulated by OSHA
• Part of SOPs

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Chemical Hazard Information
Name two sources of chemical safety information.
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Chemical Hazard Information

• Constituents of MSDS
• Health physical hazards
• Controls
• Label requirements secondary containers
• Relative to use
• Allows hazard assessment

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Chemical Hood Usage
Name three things to keep in mind when using a
chemical hood.
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Chemical Hood Usage

• Purpose
• Operational Characteristics
• Proper Hood Usage
• Common Usage Problems
• Servicing

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Laboratory Inspections
Names three things that would be deficiencies
listed on a laboratory inspection form.
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Laboratory Inspections

• POSITIVE IMPRESSIONS
• Every occupant wearing PPE
• Door has current emergency contact information
  and NFPA sign
• No food or drink containers in laboratory
• Room is clean and uncluttered
• Eyewash station is clean and labeled as being
  tested
• Chemical containers are from the 21st century
• All glassware is clean and put away when not in
  use
• All chemical containers has labels with names
  spelled out
• Centralized location for all documentation
  (Laboratory Safety Manual, MSDSs, training
  records, emergency contact info)

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Hazardous Waste Disposal
The new term used to refer to waste is _________
________.
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Hazardous Waste Handling at ISU
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Chemical Storage
Peroxide-forming chemicals must be tested how
many months after opening of the container?
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Chemical Storage

• Why is proper storage critical?
• Labeling
• General Storage Considerations
• Storage in Refrigerators
• Storage in Fume hoods
• Flammable Storage

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Hazardous Materials Shipping
Name the U.S. authority for all shipments by
land, sea and air.
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Hazardous Materials Shipping

• What materials are regulated for shipping?
• What can happen if you dont comply with the
  regulations?
• What are the steps involved in shipping?

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Biosafety
What is the name for an organism that causes
diseases in humans, plants or animals?
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Biosafety

• Recombinant DNA
• Transgenic Animals or Plants
• Bacteria, Viruses, Fungi, Prions
• Biological Toxins (e.g.Tetanus Toxin)
• Human Blood and Certain Body Fluids
• Institutional Biosafety Committee approval

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Emergency ActionsChemical Spill Procedures
Name two things you would find in an emergency
action plan.
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Emergency ActionsChemical Spill Procedures

• Types of emergencies
• Procedures
• Alarm systems
• Chemical spill issues
• Mercury
• Small versus large?
• Part of employee training

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Electrical Safety
Name two types of electrical shock injuries and
two factors impacting electrical shock.
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Electrical Safety

• Improper use of electrical equipment.
• Damaged equipment cords, plugs.
• UL-approved
• GFCI
• Vapors

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Fire Safety in the Lab
Name the three components of the fire triangle.
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Fire Safety in the Lab

• Fire Chemistry and Principles
• Controlling Lab Fire Hazards
• Fire Evacuation Procedures
• Fire Extinguishers
• PASS

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Common State Fire Marshal Fire Safety Citations -
ISU

• Hallway doors opened
• Storage lt 18 below sprinkler heads
• lt 3 clearance by electric panels
• Improper use of extension cords door wedges
• Lack of orderly and clean laboratories

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Pressure Safety
As volume increases, pressure __________ as
temperature increases, pressure __________.
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Pressure Safety

• MAWP
• Safety Factor
• Hazards of cryogens?
• Cylinders primary/secondary hazards

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Radiation Safety
Radiation that has enough energy to cause the
release of an orbital electron is called
__________.
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Radiation Safety

• Radiation Types
• Ionizing
• Non-Ionizing
• Controls
• ALARA

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Laser Safety
Name 3 ways that you can prevent exposure to a
laser beam.
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Laser Safety

• LA-SER
• Which classes of lasers are hazardous?
• Which non-beam hazard is the most significant?
• How do you protect yourself when using a laser?

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Thats It!