THE EFFECT OF PROSTAGLANDIN ANALOGS AND PROSTAMIDS ON OCULAR SURFACE IN RATS

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THE EFFECT OF PROSTAGLANDIN ANALOGS AND
PROSTAMIDS ON OCULAR SURFACE IN RATS

• Selim Doganay, MD¹ Soner Demirel, MD¹
• Iclal Gurses, MD² Mustafa Iraz, MD³
• Inonu University School of Medicine,
  Malatya-Turkey
• ¹ Department of Ophthalmology, ² Department
  of Pathology, ³ Department of Pharmacology
• Authors have no financial or proprietary interest
  in any instrument or products used in this study

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THE EFFECT OF PROSTAGLANDIN ANALOGS AND
PROSTAMIDS ON OCULAR SURFACE IN RATS

• Purpose
• To investigate the effects of prostaglandin
  analogs and prostamids on ocular surface in rats.

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THE EFFECT OF PROSTAGLANDIN ANALOGS AND
PROSTAMIDS ON OCULAR SURFACE IN RATS

• Introduction
• Long-term use of topically administered
  antiglaucomatous medication can induce
  inflammatory changes on the conjunctiva and
  corneal surface.¹
• Topical treatment may influence the success of
  glaucoma surgery.²

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THE EFFECT OF PROSTAGLANDIN ANALOGS AND
PROSTAMIDS ON OCULAR SURFACE IN RATS

• Materials and methods
• 23 Wistar albino rats were divided into four
  groups
• 0.03 bimatoprost (n6) (Group 1)
• 0.005 latanoprost (n5) (Group 2)
• 0.004 travoprost (n8) (Group 3)
• Control group (n4) received no
  treatment
• Drugs were dropped bilaterally once a day, during
  six months.

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THE EFFECT OF PROSTAGLANDIN ANALOGS AND
PROSTAMIDS ON OCULAR SURFACE IN RATS

• Materials and methods
• At the end of the six month, enucleation was
  performed then the eyes were sectioned and
  stained with hematoxylin-eosin (HE) and Massons
  trichrome for light microscopic examination.
• Dysplasia and keratinization on the ocular
  surface were evaluated.
• Number of lymphocytes and mast cells were counted
  for five adjacent high-power fields (x 400) on
  the limbal conjunctival subepithelial area.
• Collagen composition was evaluated according to
  staining density with trichrome.

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THE EFFECT OF PROSTAGLANDIN ANALOGS AND
PROSTAMIDS ON OCULAR SURFACE IN RATS

• Results
• Dysplasia was not observed on corneal and
  conjunctival epithelium.
• Corneal and conjunctival keratinization was seen
  only in bimatoprost groups (3/12) and
  latanoprost groups (1/10).

Keratinization of corneal surface epithelium (
)
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THE EFFECT OF PROSTAGLANDIN ANALOGS AND
PROSTAMIDS ON OCULAR SURFACE IN RATS

• Results
• Mean lymphocytes counts were 1.4 0.19, 2.2
  0.39, 2.27 0.33, and 1.87 0.35 in groups,
  respectively.
• There was no significant difference between all
  groups (pgt0.05).

Bimatoprost 1.40 0.19
Latanoprost 2.20 0.39
Travoprost 2.27 0.33
Control 1.87 0.35
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THE EFFECT OF PROSTAGLANDIN ANALOGS AND
PROSTAMIDS ON OCULAR SURFACE IN RATS

• Results
• Mean mast cells counts were 2.58 0.5, 5.4
  1.1, 5.7 0.58, and 3.00.59 in groups,
  respectively.
• Latanoprost groups and travoprost groups mast
  cell counts were more than others groups
  (plt0.05).

Bimatoprost 2.58 0.50
Latanoprost 5.40 1.10
Travoprost 5.70 0.58
Control 3.00 0.59
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THE EFFECT OF PROSTAGLANDIN ANALOGS AND
PROSTAMIDS ON OCULAR SURFACE IN RATS

• Results
• Mean collagen trichrome staining density scores
  were 1.0 0.85, 2.0 0.0, and 1,73 0.7 in
  groups, respectively.
• Latanaprost groups and travoprost groups
  trichrome staining density were more than
  bimatoprost groups (plt0.05).
• No difference between latanoprost groups and
  travoprost groups (pgt0.05).

Groups MeanStd. Deviation
Bimatoprost 1.000.85
Latanoprost 2.000.00
Travoprost 1.730.70
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THE EFFECT OF PROSTAGLANDIN ANALOGS AND
PROSTAMIDS ON OCULAR SURFACE IN RATS
a
b
c
d

• Controls (a), bimatoprosts (b),
  Travoprosts (c), and
• latanaprosts (d) trichrome staining.

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THE EFFECT OF PROSTAGLANDIN ANALOGS AND
PROSTAMIDS ON OCULAR SURFACE IN RATS

• Conclusions
• Long-term use of antiglaucomatous drugs may cause
  ocular surface changes.
• Chronic inflammatory changes seen in latanoprost
  groups and travoprost groups were more than
  bimatoprost groups in our study.
• The possible reason of these effects might be
  differences of preservative concentration, pH,
  and active ingredients in drugs formulation.

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THE EFFECT OF PROSTAGLANDIN ANALOGS AND
PROSTAMIDS ON OCULAR SURFACE IN RATS

• References
• 1. Broadway DC, Grierson I, OBrien C, Hitchings
  RA Adverse effects of topical antiglaucoma
  medication. I. The conjunctival cell profile.
  Arch Ophthalmol.1994112 1437-45.
• 2. Flach AJ. Does medical treatment
  influence the success of trabeculectomy? Trans Am
  Ophthalmol Soc. 2004102219-23.