The use of DNA markers to identify and explore the genetic diversity of lionfishes Pterois miles and Pterois volitans

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The use of DNA markers to identify and explore
the genetic diversity of lionfishes Pterois miles
and Pterois volitans
Rebecca M. Hamner Advisor Wilson Freshwater
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Pterois miles Species Pterois volitans
10 Dorsal Fin Rays 11
6 Anal Fin Rays 7
Shorter Pectoral Fin Size Longer
Smaller Spot size Larger
Indian Ocean, S. Africa to Red Sea, East to Sumatra Distribution West Pacific Ocean from Japan to Australia, off western Australia, South Pacific to Pitcairn Group
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P. miles P. volitans Mixed Invasive Pterois sp.
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• Current Data
• Disagreement between mitochondrial DNA
  and meristic identities
• 14 nuclear DNA loci lack species specific
  variation for P. miles and P. volitans

Act-2 AldB Cam-3 CK CKA7 ChymB-6 ITS LDH LDHA
6 Mlc-3 Ops-1 RP1 RP2 SSU
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Objective I

• Investigate hybridization using Randomly
  Amplified Polymorphic DNAs (RAPDs) to develop a
  nuclear DNA marker to distinguish between Pterois
  miles and Pterois volitans

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MethodsRandomly Amplified Polymorphic DNAs
(RAPDs)
1. Polymerase Chain Reaction
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3
4
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2
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Template DNA
Primer sites
Copied DNA fragments
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2. Gel Electrophoresis
A
?
B
Size marker
longest fragments
shortest fragments
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Objective II

• Examine the genetic diversity of North Carolina
  lionfish to determine their source population

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Methods
NC lionfish
PCR Sequence Reaction
ABI 3100 Genetic Analyzer
Phylogenetic tree or Cluster analysis
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• Questions?

Leo (NC lionfish) at CMS
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Meristic and DNA disagreement Meristic
characters not fixed for P. miles and P. volitans
Nuclear and mitochondrial DNA disagreement -Hybri
dization -Incomplete lineage sorting
Mer meristic counts Mito mitochondrial
DNA Nuc nuclear DNA
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2. Gel Electrophoresis
Partis, L. and R. J. Wells. 1996.
Identification of fish species using random
amplified polymorphic DNA (RAPD). Molecular and
Cellular Probes, 10 435-441.