Blood tubes

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Blood tubes
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Blood tubes

• Using the appropriate phlebotomy supplies is
  imperative for accurate test results.
• Each vacutainer tube is color-coded to facilitate
  proper specimen collection.
• Please be sure to use the proper tube for the
  desired test.

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Anticoagulants

• Hematology testing primarily uses whole blood
  samples (Blood colection1BW)
• Hemostatic testing uses both whole blood and
  blood plasma
• An anticoagulant is a chemical that , when added
  to the whole blood sample, prevents or delays
  clotting (coagulation) of the sample
• Regardless of the anticoagulant chosen, the
  sample and anticoagulant must be well mixed (by
  gentle inversion0 before use
• Samples that are not tested within 1 hour of
  collection should be refrigerated .

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Lavender top

• Potassium EDTA (K2 or K3 EDTA) For tests
  requiring EDTA plasma, separate plasma
  appropriately.
• Use for CBC and blood cultures

Ethylenediamine tetraacetic acid (EDTA)
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Green tops

• The interior of the tube wall is coated with
  sodium heparin, lithium heparin, or ammonium
  heparin
• The anticoagulant heparin activates
  antithrombins, thus blocking the coagulation
  cascade and producing a whole blood / plasma
  sample instead of clotted blood plus serum

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Green top

• Sodium Heparin Preferred heparin tube for send
  out testing.
• It is glass and Does Not contain inert gel
• For plasma determinations in avian and reptiles

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Lt. Green top

• Lithium Heparin Contains an inert gel for
  separating plasma, which acts as a barrier
  between cells and plasma after centrifugation.

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Red top

• Contains no anticoagulant. Used in Blood Drug
  Screens and other testing where the gel additive
  could interfere with testing.
• Red No Gel tubes are available in "No Additive"
  clot tubes as well as "Clot Activator" tubes for
  serum collection.
• Please take care to inspect the tube label to
  identify the additive type.
• Use for serum determinations in chemistry,
  serology and Immunohematology (blood banking).
• TESTS -SEROLOGY

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Red-Gray (tiger)

• Contains clot activator and inert gel for
  separating serum, which acts as a barrier between
  cells and serum after centrifugation.
• During centrifugation the barrier gel moves
  upward to the serum - clot interface, where it
  forms a stable barrier separating the serum from
  fibrin and cells.

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Important

• For tests requiring serum, a blood sample is
  drawn into a tube containing no anticoagulant.
  The blood must be allowed to clot for
  approximately 30 minutes before centrifugation.
• Clotted blood should then be centrifuged for
  10-15 minutes.
• The serum can then be separated from the cells
  and transported in an aliquot tube, if necessary.
  
• The use of a transfer pipette is the required
  method, please DO NOT tilt the original tube when
  separating the serum into an aliquot tube.
• The presence of red blood cells may give
  erroneous results

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• It is the responsibility of all personnel who
  collect laboratory specimens to properly label
  the specimens to assure accurate patient/sample
  identification.

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