CREDIT SEMINAR PHARMACOLOGY of SEABUCKTHORN (Hippophae rhamnoides) PRESENTED BY DR. MILINDMITRA K. LONARE (V-2002-30-13) DEPARTMENT OF PHARMACOLOGY

1
CREDIT SEMINARPHARMACOLOGY of SEABUCKTHORN
(Hippophae rhamnoides)PRESENTED BYDR.
MILINDMITRA K. LONARE(V-2002-30-13)DEPARTMENT
OF PHARMACOLOGY TOXICOLOGYCOVAS, CSKHPKV,
PALAMPUR-176062 (INDIA) DATE29-01-2003
2
INTRODUCTION
3
SEABUCKTHORN
4

• ? Grows naturally in cold countries on river
  sides, hill top sun facing mountains
• ? India- found in dry temporate Himalayas
• ? Himachal pradesh-found in Chamba, Kinnaur,
  Kullu Lahaul-Spiti
• ? It is quality fuel wood and fodder
• ? Potential health food

5

• Historical Background
• ? First report - China (8th century), In Yue
  Wang You Zhen medicinal classic
• ? Second report Tibetan Mongolia (18th
  century), in Sibu Yidian (Tibetan classic)
• ? India used locally since time immemorial
• ? officially listed by Chinese Pharmacopoeias
  by ministry of public health (1977)

6
Composition Functions

• Fruit juice
• 60-80 , 190 kinds of bioactive substances
• 1. Organic acids (malic/oxalic/unidentified
  acids)
• - Terminating toxicity
• - Protection from teratogenesis X-ray
  damage
• - Prevention from side-effects of O2
  therapy
• 2. Betaines (methylation of glycine)
• - prevents cures arteriosclerosis
• - anti-inflammatory

7

• 3. Triterpene (ursolic acid)
• - adrenocorticotropic mimetic
• - controls action of Na Cl-
• - anti-inflammatory activity
• 4. Flavonoids (flavonol, catechin, lukocyanidin
  flavone)
• - prevents vit-C oxidation
• - lowers cholesterol levels
• - anti-microbial anti-inflammatory
• - anti-allergic anti-tumorogenic

8

• 5. 5HT (5 - hydroxytryptamine)
• - regulates B.P. and temperature
• - influences sleep, intestinal motility,
  hormonal level emotions
• - promote coagulation
• 6. ß- amyrinoleylalcohol acid
• - dilates cardiac cerebral vessels
• - facilitates blood circulation
• 7. Coumarins
• - strengthen capillary function
• - anti-tumorogenic, anti-numbness and
  anti-pyretic

9

• 8. Triterpene (ursolic acid)
• - adrenocorticotropic mimetic
• - controls action of Na Cl-
• - anti-inflammatory activity
• 9. Amino acids Minerals
• 10. Flavoxanthin, cryptoxanthin and violaxanthin

10

• 11. Progestin
• 12. Vitamins
• - ß-carotenes (250 mg/100gm)
• - Vit.-C, K, E
• 13. Steroides (ergosterols, stigma sterols,
  lanosterols amyrins )
• 14. Tannic acid
• 15. Fatty acids

11

• Seed oil
• 8-18 , 106 bioactive substances
• 1. 22 kinds of fatty acids (saturated-13.7
  unsaturated-86.3)
• - linolenic acid (omega-3 6)
• - oleic acid- control cholesterol level
• - anti-diabetics, obesity cancer
• - essential for fat soluble vitamins

12

• 2. 6 knds of fat soluble vitamins
• - vit.A carotene-249mg/100g
• - vit.E-203mg/100g
• - vit.K-150mg/100g
• 3. 36 kinds of flavonoids and phenols
• - anti-oxidation, anti-radiation
• - enhancement of cachettin quassium functions

13

• 4. 42 kinds of phospholipids
• - strengthen the membrane mitochondria
• - participation in electron migration
• - oxidative phosphorylation cellular
  metabolism
• Fruit residue
• - 20 of total wt.
• - rich in proteins, amino acids, carotene,
  vitamin C (426-503 mg/100g), microelements

14
Nutritional value
Parts CP() EE () Ash () Cu (ppm) Zn (ppm) Fe (ppm)
Leaves 28.4 2.8 11.8 0.20 39.6 68.4
Bark 17.8 2.3 4.7 1.4 6.4 183
Branch 13.7 1.9 6.8 1.8 15.2 177
15
Seabuckthorn berries leaves
16
Effect of fodder supplementation
Animal Supplementation Effect
Weak pig Leaves Wt. gain, 9-13
Goat Leaves Inc. Milk, 6-7
Chicken Leaves, fruit, seed Wt. gain, 9-13
Layer Leaves, fruit, seed Laying cap., 26
17
PHARMACOLOGY of SEABUCKTHORN
18

• Healing potential
• Plays role in ulcer, wound and skin burn
• Causes regeneration of tissues
• Granulation of tissue formation (8 days)
• Antimicrobial activity
• Purified tannin from SBT having play role
• Having broad spectrum activity

19

• Cytoproctective Anti-oxidant activity
• CEH having capacity of anti-oxidant
• Increases elimination of free radical
• Decreases glutathione peroxidase and glutathione
  level
• Decreases cytotoxicity and apoptosis
• Restore the phagocytic activity

20

• Role in age related memory disorder
• Prevent neurological, endocrine metabolic
  effects
• Alter the level of amines
• Prevent Ach level loss

21

• Nerophysiological adaptation (Cold stress)
• Prevent cardiovascular response to cold stress
• Reduces epinephrine and norepinephrine level
• Reduces plasma cortisol level

22

• Cardiovascular diseases
• Omega-3 fatty acid decreases risk
• Decreases total and LDL cholesterol,
  triglyceroides lipoproteins
• Alter the platelet aggregation factor
• Strengthen the myocardial contraction

23

• Immune mechanism
• Prevent membrane phosphorylatio
• Prod. of Eicosanoids cytokines
• Increases serum lysosomes Alexin level
• Increases natural killer cell level
• Enhances T-lymphocyte proliferation IL-2
  secretion

24

• Anti-inflammatory activity
• Carotene, Vit. E PUFA play role
• Counter checks senilism by controlling
  proteometabolism and metabolism of nucleic acid
• Prevent damage due to radiation
• Facilitate the exudation hematopedesis
• Enhances cellular infiltration metabolism

25

• Anti-tumorigenesis
• Direct act through general immunity
• Inhibits the growth of gastric carcinoma
  lymphatic leukemia
• Blocks the synthesis of N-nitrosomolane
• Increases superoxide dismutase activity

26

• Other activities
• Protects from pain in stomach, liver, chest
  lumbar region
• Prevents hepatic damage - CCl4, alcohol
  paracetamol
• Used in gynecological ailments
• Used for treatment of cough cleaning lung
• Used as cosmetic

27

• Toxicity
• Acute chronic toxicity
• Toxic dose
• Oil 20-30 in diet
• Effects- other than vegetative CNS
• Fruit 40 in diet
• Increases bilirubin
• Decreases TLC
• Fruits 100 feeding
• Fatal to all animals

28
CONCLUSION

• Store-house of many bioactive substances
• Possesses diverse pharmacological activities
• Useful for prevention of diseases
• Has great nutritional value
• As a alternative remedy for treatment of diseases

29
THANKS