Chymotrypsin PowerPoint PPT Presentations

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chymotrypsine PowerPoint PPT Presentation
chymotrypsine - enzyme digestive utilise en industrie alimentaire
enzyme digestive utilise en industrie alimentaire
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Catalytic Mechanism of Chymotrypsin slide 1 PowerPoint PPT Presentation
Catalytic Mechanism of Chymotrypsin slide 1 - Catalytic Mechanism of Chymotrypsin 1 Chymotrypsin Protease: catalyze hydrolysis of proteins in small intestine Specificity: Peptide bond on carboxyl side of ...
Catalytic Mechanism of Chymotrypsin 1 Chymotrypsin Protease: catalyze hydrolysis of proteins in small intestine Specificity: Peptide bond on carboxyl side of ...
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Lecture 13: Mechanism of Chymotrypsin PowerPoint PPT Presentation
Lecture 13: Mechanism of Chymotrypsin - When first synthesized it is as a single polypeptide chain ... The carbonyl carbon on the substrate has 3 bonds and so is a trigonal atom. ...
When first synthesized it is as a single polypeptide chain ... The carbonyl carbon on the substrate has 3 bonds and so is a trigonal atom. ...
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Chymotrypsin Is Activated by Proteolysis PowerPoint PPT Presentation
Chymotrypsin Is Activated by Proteolysis - Chymotrypsin Is Activated by Proteolysis Chymotrypsinogen (inactive) 245 Trypsin p-Chymotrypsin (active) R15-I16 Adapted from Campbell (1999) Biochemistry (3d) p.179
Chymotrypsin Is Activated by Proteolysis Chymotrypsinogen (inactive) 245 Trypsin p-Chymotrypsin (active) R15-I16 Adapted from Campbell (1999) Biochemistry (3d) p.179
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Figure 6'01 XRay structure of chymotrypsin' PowerPoint PPT Presentation
Figure 6'01 XRay structure of chymotrypsin' - Figure 6.03 Reaction coordinate diagram for a reaction in which reactants and ... Figure 6.04 Effect of a ... Figure 6.19 Activation of chymotrypsinogen. ...
Figure 6.03 Reaction coordinate diagram for a reaction in which reactants and ... Figure 6.04 Effect of a ... Figure 6.19 Activation of chymotrypsinogen. ...
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A Multisubstrate Reaction PowerPoint PPT Presentation
A Multisubstrate Reaction - 'Ping Pong' Chymotrypsin Substrates. Chymotrypsin Kinetics Shows a 'Burst Phase' ... Crystal Structure of Chymotrypsin. Catalytic Triad Deprotonates Serine. The ...
'Ping Pong' Chymotrypsin Substrates. Chymotrypsin Kinetics Shows a 'Burst Phase' ... Crystal Structure of Chymotrypsin. Catalytic Triad Deprotonates Serine. The ...
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HHMI meeting, FOLDING PowerPoint PPT Presentation
HHMI meeting, FOLDING - MAKES REPRESSOR ACTIVE, and IT BINDS TO DNA. BIND: Immunoglobulins. Immunoglobulin. BIND TRANSFORM RELEASE: ENZYMES. Note small. active site. chymotrypsin ...
MAKES REPRESSOR ACTIVE, and IT BINDS TO DNA. BIND: Immunoglobulins. Immunoglobulin. BIND TRANSFORM RELEASE: ENZYMES. Note small. active site. chymotrypsin ...
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Formal lab report PowerPoint PPT Presentation
Formal lab report - Competitive (alter binding, raise apparent Km) ... 'Stalls' before release. Good for studying enzyme mechanisms. Chymotrypsin vs. DIFP ...
Competitive (alter binding, raise apparent Km) ... 'Stalls' before release. Good for studying enzyme mechanisms. Chymotrypsin vs. DIFP ...
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REGULATION OF ENZYME ACTIVITY PowerPoint PPT Presentation
REGULATION OF ENZYME ACTIVITY - ... ubiquitin, to targeted proteins ... that degrade proteins) like pepsin, ... Chymotrypsinogen cleavage sites to yield active chymotrypsin Calmodulin is a small ...
... ubiquitin, to targeted proteins ... that degrade proteins) like pepsin, ... Chymotrypsinogen cleavage sites to yield active chymotrypsin Calmodulin is a small ...
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Enzymes PowerPoint PPT Presentation
Enzymes - Chapter 8: Enzymes!! Properties of Enzymes. Proteins. Catalysts ... (OMP=Orotidine monophosphate involved in UMP synthesis) Proteases: e.g. ? Trypsin. Chymotrypsin ...
Chapter 8: Enzymes!! Properties of Enzymes. Proteins. Catalysts ... (OMP=Orotidine monophosphate involved in UMP synthesis) Proteases: e.g. ? Trypsin. Chymotrypsin ...
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Enzyme mechanisms PowerPoint PPT Presentation
Enzyme mechanisms - Covalent Catalysis by Enzymes. Formation of acylserine intermediate by chymotrypsin ... General acid and base catalysis. Serine proteases (esterases) all have ...
Covalent Catalysis by Enzymes. Formation of acylserine intermediate by chymotrypsin ... General acid and base catalysis. Serine proteases (esterases) all have ...
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Historical facts PowerPoint PPT Presentation
Historical facts - 1860 Pasteur L.: yeast, catalyst boud to cells ... 1930-1936 Northrop: Isolation of pepsin, trypsin and chymotrypsin in crystalline ...
1860 Pasteur L.: yeast, catalyst boud to cells ... 1930-1936 Northrop: Isolation of pepsin, trypsin and chymotrypsin in crystalline ...
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Biochemical Reaction Mechanisms PowerPoint PPT Presentation
Biochemical Reaction Mechanisms - 3. Biosynthesis of steroids from squalene. Mechanism of action of. a-chymotrypsin ... Biosynthesis of Steroids. from Squalene. Squalene (a shark oil terpene) ...
3. Biosynthesis of steroids from squalene. Mechanism of action of. a-chymotrypsin ... Biosynthesis of Steroids. from Squalene. Squalene (a shark oil terpene) ...
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Simultaneous Multiple Single Molecule Detection through Fluorescence Imaging PowerPoint PPT Presentation
Simultaneous Multiple Single Molecule Detection through Fluorescence Imaging - Most of the single molecule detection can only detect one ... Bovine a-chymotrypsin. Homodimer, has a molecular weight of 25 kD ...
Most of the single molecule detection can only detect one ... Bovine a-chymotrypsin. Homodimer, has a molecular weight of 25 kD ...
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Proteins PowerPoint PPT Presentation
Proteins - List some different functions of proteins. Describe the process of protein binding to another ... Chymotrypsin, trypsin, elastase and blood clotting proteases ...
List some different functions of proteins. Describe the process of protein binding to another ... Chymotrypsin, trypsin, elastase and blood clotting proteases ...
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Introduction to Enzymes PowerPoint PPT Presentation
Introduction to Enzymes - Chapter 13 Introduction to Enzymes Read pages 459 466 & 470 Chymotrypsin with bound substrate Enzymes are the agents of metabolic function Enzymes endow cells ...
Chapter 13 Introduction to Enzymes Read pages 459 466 & 470 Chymotrypsin with bound substrate Enzymes are the agents of metabolic function Enzymes endow cells ...
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Chapter 14 Rates of Enzymatic Reactions PowerPoint PPT Presentation
Chapter 14 Rates of Enzymatic Reactions - Reading: V&V pp. 472-487 Chapter 14 Rates of Enzymatic Reactions Chymotrypsin with bound substrate Enzyme Kinetics Several terms to know: rate or velocity rate ...
Reading: V&V pp. 472-487 Chapter 14 Rates of Enzymatic Reactions Chymotrypsin with bound substrate Enzyme Kinetics Several terms to know: rate or velocity rate ...
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Buffers PowerPoint PPT Presentation
Buffers - What effect would hypoventilating (slow and shallow breathing) have on ... Chymotrypsin Trypsin. Arg-Trp Trp-Lys. Asp Met-Phe-Asp. Lys-Arg-Met-Phe Leu-Phe-Arg ...
What effect would hypoventilating (slow and shallow breathing) have on ... Chymotrypsin Trypsin. Arg-Trp Trp-Lys. Asp Met-Phe-Asp. Lys-Arg-Met-Phe Leu-Phe-Arg ...
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Chem 415 Amino Acids and Peptides PowerPoint PPT Presentation
Chem 415 Amino Acids and Peptides - results in multiple hydrozides that are difficult to sort out. Carboxypetidase ... Tools to sequence large proteins. Asp-Pro. Mild acid. Phe Tyr Trp. Chymotrypsin. Trp ...
results in multiple hydrozides that are difficult to sort out. Carboxypetidase ... Tools to sequence large proteins. Asp-Pro. Mild acid. Phe Tyr Trp. Chymotrypsin. Trp ...
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Serine Protease and Its Inhibitor PowerPoint PPT Presentation
Serine Protease and Its Inhibitor - Title: PowerPoint Last modified by: Customer Document presentation format: Other titles: Times New Roman Arial Garamond Wingdings ...
Title: PowerPoint Last modified by: Customer Document presentation format: Other titles: Times New Roman Arial Garamond Wingdings ...
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Catalytic%20Mechanism%20of%20Chymotrypsin%20slide%201 PowerPoint PPT Presentation
Catalytic%20Mechanism%20of%20Chymotrypsin%20slide%201 - Specificity: Peptide bond on carboxyl side of aromatic side chains (Y, W, F) ... Activated H2O attacks the C=O group of the scissile peptide bond ...
Specificity: Peptide bond on carboxyl side of aromatic side chains (Y, W, F) ... Activated H2O attacks the C=O group of the scissile peptide bond ...
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Key and lock theory of induced fit PowerPoint PPT Presentation
Key and lock theory of induced fit - Pepsin. Cistein, aspartyl & metalloproteases. Bacterial termolysine. Carboxypeptidase A ... Pepsin. Pepsinogen. Stomach. Active enzyme. Zymogen. Organ ...
Pepsin. Cistein, aspartyl & metalloproteases. Bacterial termolysine. Carboxypeptidase A ... Pepsin. Pepsinogen. Stomach. Active enzyme. Zymogen. Organ ...
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Enzymes Biological Catalysts PowerPoint PPT Presentation
Enzymes Biological Catalysts - This reaction takes years without a catalyst. Some ways to increase reaction rates of ... Figure 6.17 Structure of subtilisin from Bacillus amyloliquefaciens. ...
This reaction takes years without a catalyst. Some ways to increase reaction rates of ... Figure 6.17 Structure of subtilisin from Bacillus amyloliquefaciens. ...
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Catalytic Strategies PowerPoint PPT Presentation
Catalytic Strategies - Use of molecule other than water as a proton donor or ... donating both bonding electrons to electrophile. Tetrahedral state. Acyl-E state. Acyl-E state ...
Use of molecule other than water as a proton donor or ... donating both bonding electrons to electrophile. Tetrahedral state. Acyl-E state. Acyl-E state ...
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Enzyme Behavior PowerPoint PPT Presentation
Enzyme Behavior - Hemoglobin. B. Zymogen activation. C. Enzyme phosphorylation. 1. Mechanisms of enzyme action. ... Which amino acids are located at the active site on an enzyme? ...
Hemoglobin. B. Zymogen activation. C. Enzyme phosphorylation. 1. Mechanisms of enzyme action. ... Which amino acids are located at the active site on an enzyme? ...
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Probing Mechanisms of Peptide Bond Formation PowerPoint PPT Presentation
Probing Mechanisms of Peptide Bond Formation - Probing Mechanisms of Peptide Bond Formation & Catalysis Using Models. Model of Koga ... Uses molecular recognition by a crown ether to bind a model of the ...
Probing Mechanisms of Peptide Bond Formation & Catalysis Using Models. Model of Koga ... Uses molecular recognition by a crown ether to bind a model of the ...
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Gastrointestinal function secretion, regulation PowerPoint PPT Presentation
Gastrointestinal function secretion, regulation - identical to pancreatic amylase - cleaves a-1,4-glycosidic bonds of carbohydrates ... pancreatic a-amylase - cleaves starch and glycogen into di- and ...
identical to pancreatic amylase - cleaves a-1,4-glycosidic bonds of carbohydrates ... pancreatic a-amylase - cleaves starch and glycogen into di- and ...
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Evolution by Gene Duplication PowerPoint PPT Presentation
Evolution by Gene Duplication - ... Lactate dehydrogenase (LDH), aldolase, creatine kinase ... and tyrosine. Olfactory receptors The ... size Yeast C. elegans ...
... Lactate dehydrogenase (LDH), aldolase, creatine kinase ... and tyrosine. Olfactory receptors The ... size Yeast C. elegans ...
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Protein Purification and Characterization Techniques PowerPoint PPT Presentation
Protein Purification and Characterization Techniques - Protein Purification and Characterization Techniques Nafith Abu Tarboush, DDS, MSc, PhD natarboush@ju.edu.jo www.facebook.com/natarboush Extracting Pure ...
Protein Purification and Characterization Techniques Nafith Abu Tarboush, DDS, MSc, PhD natarboush@ju.edu.jo www.facebook.com/natarboush Extracting Pure ...
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Biochemistry 6/e PowerPoint PPT Presentation
Biochemistry 6/e - Catalytic strategies What are the sources of catalytic power and selectivity of enzymes? We investigate here a specific class of enzymes: Ser-proteases.
Catalytic strategies What are the sources of catalytic power and selectivity of enzymes? We investigate here a specific class of enzymes: Ser-proteases.
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Chapter 8 (continued) PowerPoint PPT Presentation
Chapter 8 (continued) - For the midterm, you will not be tested on the following topics : ... Competitive Uncompetitive Mixed. Inhibition Inhibition Inhibition. Kmchanges while Vmax does not ...
For the midterm, you will not be tested on the following topics : ... Competitive Uncompetitive Mixed. Inhibition Inhibition Inhibition. Kmchanges while Vmax does not ...
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Regulatory Strategies for Enzymes PowerPoint PPT Presentation
Regulatory Strategies for Enzymes - Glycogen Phosphorylase. Glycogen Phosphorylase. Regulatory cascade. Hormonal signal ... Trypsinogen, chymotrypsinogen, and procarboxypeptidase are synthesized ...
Glycogen Phosphorylase. Glycogen Phosphorylase. Regulatory cascade. Hormonal signal ... Trypsinogen, chymotrypsinogen, and procarboxypeptidase are synthesized ...
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Tr PowerPoint PPT Presentation
Tr - Tr vic soustava Test pro septimu A. 6. prosince 2006 1. Chemoheterotrofov uhl k z sk vaj z anorganick ch l tek (CO2),energii ze sv tla uhl k ...
Tr vic soustava Test pro septimu A. 6. prosince 2006 1. Chemoheterotrofov uhl k z sk vaj z anorganick ch l tek (CO2),energii ze sv tla uhl k ...
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Enzyme mechanisms PowerPoint PPT Presentation
Enzyme mechanisms - Lecture 12 Enzyme mechanisms Common enzyme reactions Common enzyme mechanisms More common enzyme mechanisms Example of reactions Lysozyme Discovered by Fleming, 1922 ...
Lecture 12 Enzyme mechanisms Common enzyme reactions Common enzyme mechanisms More common enzyme mechanisms Example of reactions Lysozyme Discovered by Fleming, 1922 ...
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Biocatalysis in Organic Synthesis PowerPoint PPT Presentation
Biocatalysis in Organic Synthesis - Biocatalysed asymmetric synthesis Reduction of Carbonyl Reduction of aldehyde yields 1 alcohol. Reduction of ketone yields 2 alcohol. Reagents: ...
Biocatalysed asymmetric synthesis Reduction of Carbonyl Reduction of aldehyde yields 1 alcohol. Reduction of ketone yields 2 alcohol. Reagents: ...
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Probing Mechanisms of Peptide Bond Formation PowerPoint PPT Presentation
Probing Mechanisms of Peptide Bond Formation - Koga chemically modified the crown ether to contain 2 thiol (SH) groups to mimic ... Crown ether model demonstrates concepts of: proximity of reactive functionalities ...
Koga chemically modified the crown ether to contain 2 thiol (SH) groups to mimic ... Crown ether model demonstrates concepts of: proximity of reactive functionalities ...
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Introduction to Enzymes PowerPoint PPT Presentation
Introduction to Enzymes - Spontaneous Reactions: thermodynamically or energetically favorable ... Hydrolases: hydrolysis reactions (cleavage and introduction of water) ...
Spontaneous Reactions: thermodynamically or energetically favorable ... Hydrolases: hydrolysis reactions (cleavage and introduction of water) ...
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The two peptide lantibiotic lacticin 3147 is effective in bacteriocin induced lysis PowerPoint PPT Presentation
The two peptide lantibiotic lacticin 3147 is effective in bacteriocin induced lysis - The two peptide lantibiotic lacticin 3147 is effective in bacteriocin induced lysis Department of Dairy Science and Technology, Instituto del Fr o (CSIC), Ciudad ...
The two peptide lantibiotic lacticin 3147 is effective in bacteriocin induced lysis Department of Dairy Science and Technology, Instituto del Fr o (CSIC), Ciudad ...
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Chapter 14 Mechanisms of Enzyme Action PowerPoint PPT Presentation
Chapter 14 Mechanisms of Enzyme Action - Chapter 14 Mechanisms of Enzyme Action Aspartic proteases play many roles in humans The Aspartic Proteases Pepsin, chymosin, cathepsin D, renin and HIV-1 protease All ...
Chapter 14 Mechanisms of Enzyme Action Aspartic proteases play many roles in humans The Aspartic Proteases Pepsin, chymosin, cathepsin D, renin and HIV-1 protease All ...
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Effective Mentoring of Undergraduate and Graduate Students PowerPoint PPT Presentation
Effective Mentoring of Undergraduate and Graduate Students - Title: PowerPoint Presentation Author: Carla Mattos Last modified by: QEM Created Date: 1/7/2002 6:13:24 PM Document presentation format: On-screen Show
Title: PowerPoint Presentation Author: Carla Mattos Last modified by: QEM Created Date: 1/7/2002 6:13:24 PM Document presentation format: On-screen Show
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Enzyme Catalysis-Serine Proteases PowerPoint PPT Presentation
Enzyme Catalysis-Serine Proteases - Enzyme Catalysis-Serine Proteases Concepts to be learned Activation Energy Transition State Example: Proteases Requirements for proteolysis Families of proteases
Enzyme Catalysis-Serine Proteases Concepts to be learned Activation Energy Transition State Example: Proteases Requirements for proteolysis Families of proteases
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2'1' Les enzymes, acteurs du mtabolisme PowerPoint PPT Presentation
2'1' Les enzymes, acteurs du mtabolisme - Les acides amin s du site actif du lysozyme au niveau tridimensionnel (a) et au niveau de la structure primaire (b) R action de l'aspartate transcarbamylase ...
Les acides amin s du site actif du lysozyme au niveau tridimensionnel (a) et au niveau de la structure primaire (b) R action de l'aspartate transcarbamylase ...
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Development of an in vitro Assay to Measure Intestinal Protein Digestion and Why PowerPoint PPT Presentation
Development of an in vitro Assay to Measure Intestinal Protein Digestion and Why - Development of an in vitro Assay to Measure Intestinal Protein Digestion and Why Debbie Ross and Mike Van Amburgh Dept. of Animal Science Feed Library Updates to Pool ...
Development of an in vitro Assay to Measure Intestinal Protein Digestion and Why Debbie Ross and Mike Van Amburgh Dept. of Animal Science Feed Library Updates to Pool ...
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Seminars this week for credit: PowerPoint PPT Presentation
Seminars this week for credit: - Seminars this week for credit: ... 4:00 p.m. SL 110. Peggy Daley. Final Exam. W December 13. 10:30 am ... 2) active site modification (irreversible inhibitors) ...
Seminars this week for credit: ... 4:00 p.m. SL 110. Peggy Daley. Final Exam. W December 13. 10:30 am ... 2) active site modification (irreversible inhibitors) ...
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Protein Purification and Characterization Techniques PowerPoint PPT Presentation
Protein Purification and Characterization Techniques - Size-exclusion chromatography Gel-filtration chromatography Separation on the basis of size (MW) Stationary (cross-linked gel particles): ...
Size-exclusion chromatography Gel-filtration chromatography Separation on the basis of size (MW) Stationary (cross-linked gel particles): ...
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Seminars this week for credit: PowerPoint PPT Presentation
Seminars this week for credit: - http://video.nbc.com/player.html?dlid=45139. Chapter 15. Catalytic Mechanisms ... 2) active site modification (irreversible inhibitors) ...
http://video.nbc.com/player.html?dlid=45139. Chapter 15. Catalytic Mechanisms ... 2) active site modification (irreversible inhibitors) ...
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From Structure to Function PowerPoint PPT Presentation
From Structure to Function - From Structure to Function
From Structure to Function
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ENZYMES KINETICS, INHIBITION, REGULATION PowerPoint PPT Presentation
ENZYMES KINETICS, INHIBITION, REGULATION - ENZYMES KINETICS, INHIBITION, REGULATION Muhammad Jawad Hassan Assistant Professor Biochemistry ...
ENZYMES KINETICS, INHIBITION, REGULATION Muhammad Jawad Hassan Assistant Professor Biochemistry ...
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Strategies in Enzyme Catalysis PowerPoint PPT Presentation
Strategies in Enzyme Catalysis - Strategies in Enzyme Catalysis ... Covalent catalyis (nucleophilic or ... a nucleophilic amino acid attacks the phosphate, releasing fluoride anion. ...
Strategies in Enzyme Catalysis ... Covalent catalyis (nucleophilic or ... a nucleophilic amino acid attacks the phosphate, releasing fluoride anion. ...
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Review Session: Monday, Dec' 8 PowerPoint PPT Presentation
Review Session: Monday, Dec' 8 - Saint-Saens, Berlioz. and some French carols. Figure 14-15 Effect of pH on the initial rate of the reaction catalyzed by the enzyme fumarase. ...
Saint-Saens, Berlioz. and some French carols. Figure 14-15 Effect of pH on the initial rate of the reaction catalyzed by the enzyme fumarase. ...
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Animal nutrition PowerPoint PPT Presentation
Animal nutrition - Animal nutrition Chapter 41 Fig. 41-15b Microvilli (brush border) at apical (lumenal) surface Key Nutrient absorption Blood capillaries Epithelial cells Villi Lymph ...
Animal nutrition Chapter 41 Fig. 41-15b Microvilli (brush border) at apical (lumenal) surface Key Nutrient absorption Blood capillaries Epithelial cells Villi Lymph ...
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Animal nutrition PowerPoint PPT Presentation
Animal nutrition - Animal nutrition Chapter 41
Animal nutrition Chapter 41
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Enzyme PowerPoint PPT Presentation
Enzyme - ???????????????????????? ?????????????? (Ribozyme or Catalytic RNA) ... ????????????? Hydrolysis. Esterases. Proteases. Phosphatases. Amylases. Peptidases. Ureases ...
???????????????????????? ?????????????? (Ribozyme or Catalytic RNA) ... ????????????? Hydrolysis. Esterases. Proteases. Phosphatases. Amylases. Peptidases. Ureases ...
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Chapter 6 Protein Function : Enzymes Part 1 PowerPoint PPT Presentation
Chapter 6 Protein Function : Enzymes Part 1 - Chapter 6Protein Function : Enzymes Part 1. This part will get into Enzyme nomenclature and kinetics (rates of catalysis).
Chapter 6Protein Function : Enzymes Part 1. This part will get into Enzyme nomenclature and kinetics (rates of catalysis).
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Enzymes : PowerPoint PPT Presentation
Enzymes : - involves basic principles of organic chemistry ... after mainly aromatic amino acids, while trypsin cleaves after basic amino acids. ...
involves basic principles of organic chemistry ... after mainly aromatic amino acids, while trypsin cleaves after basic amino acids. ...
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Regulation of Enzyme Systems PowerPoint PPT Presentation
Regulation of Enzyme Systems - systems are controlled by. regulating some of their enzymes. Types of Regulation ... processed into active enzymes; pieces of. polypeptide removed- IRREVERSIBLE ...
systems are controlled by. regulating some of their enzymes. Types of Regulation ... processed into active enzymes; pieces of. polypeptide removed- IRREVERSIBLE ...
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