Isoelectric PowerPoint PPT Presentations

Title: 5 Author: kayxiong Last modified by: kayxiong Created Date: 8/29/2004 1:27:56 PM Document presentation format

GEL ELECTROPHORESIS Gel electrophoresis uses a ... cellulose acetate paper electrophoresis Slide 16 Gel Electrophoresis AGAROSE GEL POLYACRYLAMIDE GEL DNA ...

Biochemistry

They all cause horizontal streaks. Underfocusing: insufficient focusing Vh. ... (pI change and start moving again.streak) Basic narrow gradients are sensitive to ...

PROTEOMICS: LARGE-SCALE PROTEIN IDENTIFICATION & ANALYSIS 2D-gel electrophoresis & mass spectrometry 1. Isolate proteins from tissue (organism, condition ) of interest

The proteins that are to be used: Ovalbumin. Casein. Gluten. Theoretical Background ... Gluten = 7.64. Blank result in our film. Possible problems. Solutions ...

Some Commonly Used Gel Filtration Materials. Purification of Rat Liver Glucokinase. SDS-PAGE sample results. Isoelectric Points of Several Common Proteins. 2D ...

Charge Ion Exchange Chromatography Native gel electrophoresis Isoelectric focusing Charges on proteins Different proteins have different native charges.

Zeta potential at break between Stern and diffuse layers ... 2.3 RT F-1 (pCpzc- pC) Proton Surface Charge. pHpzc. Zeta Potential. Isoelectric Point ...

Molecular Weight. Amino Acid Frequency. Isoelectric Point. UV ... Molecular Weight & Proteomics. 2-D Gel QTOF Mass Spectrometry. Amino Acid Frequency ...

isoelectric point, hydrophobicity) Secondary Structure ... Hydrophobicity is a property of groups of amino acids - best examined as a graph ...

Quality Control Biochemistry IEF (Isoelectric Focusing) HPLC (High Pressure Liquid Chromatography) ELISA (Enzyme-Linked Immunosorbent Assay) SDS-PAGE (Sodium Dodecyl ...

Electrophoresis. Separate based on size and shape. Fluorescence ... 2D Gel-Electrophoresis. Protein separation. Molecular weight (Mw) Isoelectric point (pI) ...

Casein is the protein found in all mammals’ milk which include cow, sheep, goat, yak, buffalo, camel and human. Casein also exists in pure form by acidifying milk to the isoelectric point at pH 4.6 by either lactic acid or mineral acid.

alanine (R = CH3) Ala A. phenylalanine (R = CH2C6H5) Phe F. tyrosine (R = CH2C6H4OH) Tyr Y ... Each amino acid has an isoelectric point, (pI) numerically equal ...

Isoelectric point (pI) Staining. Birds-eye view of protein abundance. 2D Gel-Electrophoresis ... mass spectrometry doesn't discriminate against novel peptides...

Protein sequences access gene sequences and are key to molecular biology ... separates based on molecular weight and/or isoelectric point. 10 fmol - 10 pmol ...

Best care for your children with CDG. PHYSICIANS. Provide the ... More defects being found all the time. Isoelectric focusing of transferrin. 4. 2. 0. control ...

20 natural a-amino carboxylic acids. 19 are primary amines, proline ... When pH is equal to the pI (isoelectric point) amino acids exist in the zwitterion form. ...

Gel Electrophoresis Steps Breakdown the protein into amino acids Break amide/peptide bond Called hydrolysis (opposite of condensation) Acid or base and heat required ...

Biochemistry

Title: PowerPoint Author: Handsome Yu Last modified by: Created Date: 10/26/2001 12:27:17 AM Document presentation format:

Title: BC basic - from big bang Author: Rong-Huay Juang Last modified by: juang Created Date: 8/15/1997 8:46:50 PM Document presentation format:

Proteins, Amino Acids, and pH. Weak Base. Weak Acid. Amino acid as a zwitterion. If tyrosine is buried deep inside a protein, it is not readily accessible and a ...

(CZE) capillary zone electrophoresis (CZE) capillary gel electrophoresis

Amino Acids (Chapter 37B) General Structures. Two contrasting groups -COOH acidic ... Less acidic and basic than normal RCOOH and RNH2. COO- C H3N. H. R ...

Chapter 09 Polyprotic Acid-Base Equilibria Proteins perform biological functions such as structural support, catalysis of chemical reactions, immune response to ...

NUTRITION

Zwitterion ... dipolar ion / zwitterion. The dipolar nature of amino acids give to. their ... zwitterion is electrically neutral and exists at specific pH. ...

II- Classification according to polarity of side chain (R): A- Polar amino acids: in which R contains polar hydrophilic group so can forms hydrogen bond with H2O.

According to the latest research report by IMARC Group, The global protein purification and isolation market size reached US$ 9.2 Billion in 2023. Looking forward, IMARC Group expects the market to reach US$ 19.4 Billion by 2032, exhibiting a growth rate (CAGR) of 8.4% during 2024-2032. More Info:- https://www.imarcgroup.com/protein-purification-isolation-market

Molecular mass determination by gel filtration chromatography ... Logarithmic relationship between the molecular mass of a protein and its ...

... Protein Structure Forces Involved in Protein Structure Primary Structure of Insulin Primary Structure of Lysozyme -Helix Secondary Structure -Structure ...

Polyprotic Acid-Base Equilibria Introduction 1.) Polyprotic systems Acid or bases that can donate or accept more than one proton Proteins are a common example of a ...

Polyprotic Acid-Base Equilibria Introduction 1.) Polyprotic systems Acid or bases that can donate or accept more than one proton Proteins are a common example of a ...

Fractional composition clearly show the dominant and minor species in solution. ... Draw the structure and the fractional composition of the principal species of 1, ...

Centrifuge the sample to separate the insoluble portion (pellet) from the soluble (supernatant) Remove a portion of the supernatant and mix with 'gel' loading buffer ...

General method for synthesis of azo dyes Add a cold aqueous solution of sodium nitrite slowly (with cooling and stirring) to a cold solution of the amine compound in ...

biochemical methods used in proten purification and characterization biochemical methods used in proten purification and characterization classical methods for ...

CHE 242 Unit VIII The Structure, Properties, Reactions and Mechanisms of Carboxylic Acids and Their Derivatives CHAPTER TWENTY-FOUR Terrence P. Sherlock

This is a method for the separation and identification of proteins in a sample ... http://www.aber.ac.uk/parasitology/Proteome /Tut_2D.html#Section 3 ...

Possible transfer of vanomycin resistance genes to more serious pathogens is a major concern. ... Peptides are separated by hydrophobicity on reverse phase column ...

MODULE 6

Amino acids are zwitterion molecule with both positive and negative charge ... Neutral zwitterion. Only ions are H2A , A-, H and OH ...

Title: Lecture note : CE Subject: capillary electrophoresis Author: dslee Last modified by: Created Date: 8/25/2001 1:43:34 AM

Protein Purification and Characterization Techniques Nafith Abu Tarboush, DDS, MSc, PhD natarboush@ju.edu.jo www.facebook.com/natarboush Extracting Pure ...

An anion-exchange resin has positive charge and binds negatively charged ... Anions of SDS bind to peptide chain and protein is negatively charged, moves to anode. ...

Naturally occurring amino acids has an amino group (NH2) to the carboxyl group (COOH) ... Since it exists as internal salt, known as zwitterion, ...

Introduction: Protein Aulanni am Laboratorium Biokimia Jurusan Kimia FMIPA Universitas Brawijaya

Physical adsorption - van der Waals forces. Electrostatic adsorption ... Cation and Anion Adsorption. Cations. Anions. 0. 100. pH % adsorbed. Sorption edge ...

In proteins only L- configuration is found ... Zwitterion: 'hybrid ion' can act as acid (proton donor) or base (proton acceptor) ...

The relative mobility is almost a near linear function of the molecular weight. ... bands are observed of lower molecular weight, then we have a multisubunit ...

Hydrophobicity. Hydrophobic residues usually are buried internally ... hydrophobic interaction(HIC) hydrophobicity. DNA affinity DNA binding site ...

ELECTROPHORETIC METHODS Commonly used protein stains An ethidium-stained gel photographed under UV light Western blott technique Commonly used protein stains An ...

name given to a particular family of separation techniques ... Elution analysis. Frontal analysis. Displacement development analysis ...

Overview of 2DE Complex mixture of proteins Denature and solubilize in solution (Sample prep) Separate proteins by charge in first dimension (IEF)