Morpholinos PowerPoint PPT Presentations

Title: Morpholinos Author: Jon D. Moulton, Ph.D. Last modified by: Jon Moulton Created Date: 9/1/2005 4:40:01 PM Document presentation format: On-screen Show

Morpholinos Mary Mullins, UPenn 25-mer antisense oligonucleotide with modified bases containing morpholine ring, making them very stable. How do they work?

... about the functions of proteins in cells. ... Cells make proteins ... The order of the DNA bases determines the order of amino acids in a protein. RNA. RNA ...

In the nucleus: DNA is copied making mRNA and introns are removed ... syndrome cannot be displaced from methylated DNA by notch in Xenopus embryos. Mol Cell. ...

Morpholinos are now available for highly selective gene knockdown in zebrafish. ... Zebrafish = 3 months to maturity after fertilization ...

INHIBITORS What are some examples? What experiments show they are SPECIFIC!!! Do they always work? INHIBITORS Used to diminish or abolish a biological activity so ...

Cell Migration During Embryonic Development

Automated Segmentation and Classification of Zebrafish Histology Images for HighThroughput Phenotypi

The Characterization of RhoGEFs and The Role of NET1 in Zebrafish Gastrulation ... The Gastrulation Period of Zebrafish Embryonic Development ...

Molecular tool box for studying gene function in neural development For Eye Development Course Studying gene function in neural development Investigation of gene ...

CAT: radioactive assay. LacZ: colorimetric assay/staining. GFP: ... GH-transgenic Arctic charr: cellular growth of skeletal muscle. What process was stimulated? ...

... pools to find individual cDNAs: find noggin. Expressed in right place, time ... Expression cloning of noggin, a new dorsalizing factor localized to the Spemann ...

A is necessary for B to occur! How can we test this? we have an assay for B ... Zebrafish (transparent embryos) - macrophages exit blood vessel, head right for wound ...

The polarity of the animal-vegetal axis in a Xenopus embryo is first decided ... d) vegetal hemisphere cells in this region receive signals from overlying animal ...

... selective growth medium (no neomycin or gancyclovir) the most frequent ... 6) In medium containing neomycin and gancyclovir, the most frequent class will be ...

Freimann Life Science Center Center for Zebrafish Research RESEARCH CONTRIBUTION HIGHLIGHT OVERVIEW LIST OF SERVICES Freimann Life Science Center (FLSC)

The drug Sacituzumab govitecan-hziy is used to treat patients with unresectable locally advanced or metastatic triple-negative breast carcinoma and locally advanced or metastatic urothelial carcinoma by healthcare professionals. The active ingredient in the medicine, Sacituzumab govitecan-hziy, is a Trop-2-directed antibody and topoisomerase inhibitor conjugate. The recombinant monoclonal antibody (Sacituzumab govitecan-hziy) is produced by mammalian cells, while the small molecule components SN-38 and CL2A are produced via chemical synthesis. This medicine is supplied in 180 mg in single-use vials for intravenous infusion. Trodelvy 180 mg injection is readily available from several generic medicine suppliers. To know more about this drug, dial 1800-889-1064.

Differential RNA processing generates proteomic ... Unc-22 null phenotype also seen in progeny (!) of injected worms ... Arrow, miR165-guided cleavage site. ...

imp mos in biotechnology industries

Title: PowerPoint Presentation Last modified by: user Created Date: 1/1/1601 12:00:00 AM Document presentation format: Other titles

Title: PowerPoint Presentation Author: YourName Last modified by: Aartsma-Rus, A.M. (HG) Created Date: 1/22/2004 2:47:02 PM Document presentation format

Systems biology: identification of regulatory regions and disease causing genes and mechanisms PhD defense Peter Van Loo Promotor: P. Marynen Co-promotors: B. De Moor

studies on miRNA biogenesis and methods of spatiotemporal and tissue-specific regulation. ... Could still be minor alternative biogenesis pathway(s), such as a ...

Section C - Properties of Nucleic Acids Contents C1 Nucleic Acid Structure Bases, Nucleosides, Nucleotides, Phosphodiester bonds, DNA/RNA sequence, DNA double helix ...

Canadian Bioinformatics Workshops www.bioinformatics.ca

Ebola vir s hastal (Ebola hemorajik ate i), bir hemorajik ate vir s olan Ebola vir s n etken oldu u klinik antitedir. Rift Valley fever

Title: Modern Methods in Drug Discovery Subject: Target Identification and Animal Models Author: Michael Hutter Last modified by: Michael Hutter Created Date

By Adam Spofford Blue Science 630s Ebola virus is a complex virus that destroys the walls of arteries and some other connective tissue. There are five known types ...

Zebrafish as a

Strategy:

Usually, we are interested in how much acid or base is present in a sample ... Ribonuclease enzyme is composed of 124 amino acids. ...

Long before Pasteur and K ch works on pathogenic bacteria Gruby and Sch nlein ... But medical mycology was neglected under the shadow of medical bacteriology, ...

in the era of genomics there is an even greater need than before for ... been used in a variety of organisms including sea urchin, Xenopus, chick, and mouse ...

Alternative 5' splicing. Unwanted = longer exon. Antisense shorter isoform ... B. Bias alternative splicing ratios. Target the unwanted isoform exon-intron joint. ...

It also contains links to the example sequence files used in the workshop, and ... There should be two sequences: surfeit1' for frog and fly. ...

Utility and use of zebrafish as model for understanding angiogenesis'

The Expression Profile of NET1 in WT zebrafish embryos (a) The Expression Profile of RhoGEF: ... 50% Epiboly Zebrafish treated with 8ng NET1-long Morpholino ...

Fig. 1: Zebrafish Genetics Laboratory located at room 210 of Nursing building is ... Fig. 3 The recapitulation of reporter GFP pattern with endogenous Nav1.6 ...

Development of Complementary Animal Models to the Classic Rodent Assays for Determining Neurotoxic E

'The receptor protein-tyrosine phosphatase, Dep1, acts in arterial/venous cell ... Beuchle D, Schach U, Fabian C, N sslein-Volhard C. The identification of genes ...

Comparative Analysis of Human Chromosome 22q11.1-q12.3 with Syntenic Regions in the Chimpanzee, Baboon, Bovine, Mouse, Pufferfish and Zebrafish Genomes

Several genes, whole animal phenotype, protein product (GFP), RNA level (in situs) ... Much of this evidence is from EST database analysis ...

cloning (aka somatic nuclear transfer): taking the nucleus from one cell ... Gurdon furthered the idea of cloning by culturing epithelial cells, and ...

Une pizootie de grippe aviaire est observ e depuis d cembre 2003. ... Diagnostic ais seulement en p riode pid mique. Une seule solution : diagnostic ...

The microtubule connection:

71 71, 73, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 89, 92, 93, 97 ... plus hermit crab. leech (annelid worm) Chordate. Cnidarian. Porifera (sponges) ...

Gene A is expressed maternally and zygotically from stages 10-30 during Xenopus development ... Transgenic Xenopus (efficient procedure, vertebrate embryo) ...

Inhibition of topoisomerases a family of enzymes responsible for maintaining ... Torsades de pointe: paroxysm of ventricular tachycardia in which the ...

We identified a zebrafish caudal-related homeobox (cdx1b) gene which shares ... in intron 1 of the foxa2 gene based on an electrophoretic mobility shift assay. ...

Managed the team in charge of the Fugu genome annotation in Singapore ... Focus on all chordate genomes, include Chicken, Fugu, Zebrafish, Ciona i., Ciona s. ...

In an ideal world, the color change would happen when you mix the two solutions ... Total moles of OH- in the solution: 50.00 10-3 L 0.0200 M = 1.00 10-3 moles of OH ...

Tweaking BLAST. Although you normally see BLAST as ... of parameters, but most are way too obscure even for die-hard techies like me! ... 4. Limit Entrez query ...

Center for Zebrafish Research (CZR) Housed in approximately 3,000 square feet ... A separate room houses the zebrafish nursery ...