... Z Y A LacI lac Operon RNA Polymerase araC ara GFP Operon GFP Gene araC GFP Gene araC GFP Gene Effector (Arabinose) B A D araC B A D araC RNA Polymerase ...
Study of biological processes (example: synthesis of proteins) ... Skin, Eyes and Organs give off an eerie light. Only fur does not glow. Column Chromatography ...
... gene expression Cell movement Cell fate during development Formation of different organs Screenable marker to identify transgenic organisms What is Transformation ...
pGLO & GFP pGLO Transformation and Purification of Green Fluorescent Protein (GFP) Instructors Stan Hitomi Director, Edward Teller Education Center UC Davis ...
Helpful Hints: Hydrophobic Interaction Chromatography Add a small piece of paper to collection tube where column seats to insure column flow Rest pipet tip on side of ...
QUICKLY REPLACE LIDS FLIP PLATES UPSIDE DOWN STACK AND TAPE LABEL WITH YOUR GROUP NAME PLACE IN INCUBATOR pGLO plasmid bla (beta-lactamase) - On all time - Makes ...
Instructors Stan Hitomi Coordinator Math & Science San Ramon Valley Unified School District Danville, CA Kirk Brown Lead Instructor, Edward Teller Education ...
Instructors Sherri Andrews, Ph.D. Curriculum and Training Specialist Bio-Rad Laboratories Essy Levy, M.Sc. Curriculum and Training Specialist Bio-Rad Laboratories ...
Analyze how a gene can transform an organism and express that gene ... Bacteria present with whitish colonies (regeneration of the starter plate) -pGlo. LB only ...
Bacterial Transformation of pGLO Lab Background Gene Regulation Review Organisms regulate expression of their genes and ultimately the amounts and kinds of proteins ...
Bacterial Transformation of pGLO Lab Background Gene Regulation Review Organisms regulate expression of their genes and ultimately the amounts and kinds of proteins ...
Plasmids and Vectors Instructor Supplement to pGlo Bacterial Transformation A more detailed look at plasmids Cloning into a Plasmid Asilomar Conference Screening ...
... you tense a muscle or waggle a finger; whenever your heart pounds out a beat, ... What makes jelly fish glow? What makes jelly fish glow? 7/23/09. Helwig ...
GFP Transformation Lab Images taken without permission from http://upload.wikimedia.org/wikipedia/de/4/4d/Protein_GFP_1EMA.png, http://bioinfo.biotec.or.th/Picture ...
A circular piece of autonomously replicating DNA. What is a plasmid? ... Electrical shock makes cell membranes permeable to DNA. Calcium Chloride/Heat Shock ...
This Little Light of Mine: Transform bacteria with a Jellyfish. gene to make them glow ... Aequorea victoria jellyfish. araC gene. Regulates GFP transcription. ori ...
... of LDH Protein structure Km of enzymes 3 Purification by pH Column Chromatography Precipitation of pro tein and Affinity ... Chromatograph Separation by size ...
Each strand of a DNA molecule is oriented in an opposite fashion ... (2) Euks have many different sites of origin -- bi-directional. Back to the Board. QDFT 100 ...
The genus name is a combination of arrangement + shape. ... the bacteria that causes botulism the bacteria that causes tetanus Artist, Charles Bell, ...
Observations of Multiple Transformation Kits Bryan Smith (LaLumiere School) Teresa Pairitz (Marian High School) Shelly Gregory (South Bend Adams High School)
Baculovirus: an insect cell virus, particularly useful for study of eukaryotic proteins ... Load supernatant. Load gels and perform electrophoresis ...
Recombinant DNA technology utilizes the power of ... Protoplast fusion ... Protoplast fusion. Nocardia lactamdurans produces the antibiotic cephalomycin ...
Human Growth hormone inserted into the embryo of the mouse on the left. ... for land mines as seeds would be spread widely and evenly in a suspect field ...
Biotechnology and Recombinant DNA. Terminology. Biotechnology. Genetic engineering ... DNA fingerprinting. Nanotechnology. Bacteria produce tiny pieces of metal ...
Bacterial Genetics (Ch18) Genetic diversity caused by Rapid reproduction Mutation Recombination Bacteria One of the simplest genetic model systems to study the ...
Biotechnology and Genetic Engineering AP Biology Chapter 20 * Figure 20.6 Making complementary DNA (cDNA) for a eukaryotic gene * Figure 20.6 Making complementary DNA ...
Biotechnology and Genetic Engineering AP Biology Chapter 20 * Figure 20.6 Making complementary DNA (cDNA) for a eukaryotic gene * Figure 20.6 Making complementary DNA ...
... 1,4-b links are more easily hydrolysed by acid catalysts 1,4-b links make the polysaccharide chain particularly compact ... l empilement des pores est ...
... 4 before reading each section on volcanoes. ... to United Streaming Videos on Volcanoes. ... Fill in this Volcano diagram using the definitions on the bottom ...
Biotechnology. bios = life technos = tool ... Murder case in Phoenix, Arizona ... XVIII (child king who died in prison) compared to hair from Marie Antoinette ...
Ch 12 History of Life on Earth * * * * * * * * The Earth formed about 4.5 billion years ago The Earth was a fiery ball of molten rock that cooled, forming an outer ...
Incorporation of Molecular Biology, Bioinformatics, Genomics ... New bioinformatics exercises will be. based on web-based sources, or. downloadable software ...
Horizontal Gene Transfer and Genetic ... occurs when prophage is incorrectly excised Specialized transduction Recombination and Genome Mapping in Viruses viral ...
Bacterial Genetics Bacteria are essential organisms in demonstrating structure and function of DNA: Their haploid genetic state allows expression of a genetic trait ...
The 'key' to survival. The total of all the life activities required to ... transformation worked because we grew the bacteria on plates full of ampicillin. ...
Circular piece of autonomously replicating DNA. What is a plasmid? ... Add pGLO plasmid DNA to ' ' tube. Incubate tubes 10 minutes on ice. Heat shock 50 seconds ...