... transfectants of BHK cells (BHK = baby hamster kidney) Works: ... Mouse and hamster cell lines used for commercial ... data available for Chinese hamster ...
... gene expression, temperature sensitivity, chemical resistance, et ceterus ... PCR-based Gene Engineering. 40-50bp homology arm. each side. Alleles and Phenotypes ...
a) all will be variegated (both black and white patches of coat color) ... 6. You mate a variegated (chimeric) female mouse to a white male, and examine ...
... mouse. Creating the KO mouse using ES cells. Mother will give birth to a range of mice. ... Cre-Lox Technology- Application in the mouse. Need 2 different mice ...
Remember that we will start the morning. by voting on the identify of mystery strains ... Activating (gain-of-function) mutations aid epistasis analysis ...
http://saturn.roswellpark.org/huberman/BIR572/YeastGeneticsLectureNotes.htm l ... Meiosis I. Meiosis II. from 'Getting Started with Yeast' by Fred Sherman ...
chemotherapy resistance (MDR, P-glycoprotein, efflux pump) cancer (myc, ras) In biotechnology: high level recombinant protein production in mammalian cells. 22 - 3 ...
Correlate identified chromosome loss. With loss of phenotypic trait (isozyme, DNA sequence, etc. ... Recombination between tandem inserts (higher freq). 9/28/09. 8 ...
Use parafilm or old xray film to create a 'mask' Plenty of paper towels. Time ... Variations include FISH. Applications of a Northern blot. mRNA detection ...
Well characterized genetic system. Well characterized genome-basically sequenced and annotated. ... Worm's brain. White et al. Phil. Trans. Royal Soc. London ...
Transfer of the growth-transformed phenotype: ability to grow in multilayers or ... (ASF) double gene K.O. in chick DT40 lymphoid cells (high rate of homologous ...
Wang et al.: double K.O. of splicing factor gene; strategy, coverage of viability ... in cultured lines: myc double K.O. = viable, sick (Skip?) 23. Or (50:50) ...
(so analyze a pool of many to. Super-physiological expression? ... Tritium grains from hybridized cDNA. HSR: Homogenously. staining. region. Gene amplification ...
Overhangs re-anneal. Removal of 5' Phosphate. Prevents re-ligation of vector ... cloning or using a single restriction site on the vector where it may re-anneal ...
Genetics as a subject (genetic things that go on in somatic cells: ... Recessive mutations (most knock outs) are masked. 10/19/09. 4. Solutions to diploidy problem: ...
bioinformatics on related organisms (conserved genes) or clone by phone -Reverse TLN: aa with minimal redundancy reversed into DNA sequence (genetic code ...
GHT DAP GHT. Only mutation. GHT = glycine, hypoxanthine, and thymidine. A = adenine ... H DAP H. Only mutation. Originally intended table: Red = corrected ...
LOCALIZATION OF A MOLECULE - placing in space and or time.-Answering where and/when. Localize with respect to WHAT? TIME - embryogenesis, cell cycle, post stimulation ...
... Variable region * OUTLINE OF NEXT LECTURE TOPICS Expression and manipulation of transgenes in the laboratory In vitro mutagenesis to isolate variants of your ...
E. coli as a host PROs: Easy, flexible, high tech, fast, cheap; . . . . . . but problems CONs Folding (can misfold) Sorting - can form inclusion bodies
... same considerations apply for the creation of double knockout recessive tumor ... Mutagen target specificity (a particular base or base combination (e.g., GG) ...
Medicinal Chemistry Donlene Webb SMU Viruses A virus is a submicroscopic obligate parasitic particle that infects cells in biological organisms. Viruses are non ...
Genome Engineering. Goal: Manipulate pieces of DNA to build recombinant ... Zebrafish -REMI -Transposase systems working and gaining popularity. Mouse ...
... Gene yfp-Your Favorite Protein Phage display links variant gene to the protein it specifies Libraries screened by affinity 1011 variants ... Enrichment ...
Less expensive than protein production of cells in culture. Similar method to the method of transgenic mice ... To facilitate the optimal virus harvest time ...
Title: PROGENI Enrollment Actual vs Projected Author: Chalter Last modified by: Mohammad Al-Khateeb Created Date: 5/11/2000 2:09:20 PM Document presentation format
Incubate 2-3 days. Class 15. 16. In vitro translation following in vitro transcription ... various others, many differentiated to different degrees. AND: ...
Name any one of 2 methods for getting 500 ug of a human gene of your choice ... you had only the figure of the Hind III digest shown at right, and knew nothing ...
... Tet gene. Note A single cut linear could self ligate...this would still be Tet ... This is what you want (Tet sensitive) with insert, but, you still have to ...
The polarity of the animal-vegetal axis in a Xenopus embryo is first decided ... d) vegetal hemisphere cells in this region receive signals from overlying animal ...
One Cell is Enough to Break Tor s Anonymity Xinwen Fu University of Massachusetts Lowell Team members Zhen Ling, Southeast University Junzhou Luo, Southeast University
Application to massively parallel sequencing of genomes, transcriptomes, SNP ... Juhl, Kenneth W. Kinzler, and Bert Vogelstein pp.16368 16373 PNAS November ...
... of Health and Family Welfare. Department of Health. Nirman Bhavan, New Delhi ... Animal health and related issues: Director (Trade) (Shri S. K. Srivastava) ...
... selective growth medium (no neomycin or gancyclovir) the most frequent ... 6) In medium containing neomycin and gancyclovir, the most frequent class will be ...
Select for a phenotype and recover the gene from bacterial or phage clone ... Positional Cloning. ... This method works also for transposon based cloning...
... that were in equal amounts in DNA, repeated over and over tetra-nucleotide ... X-ray crystalography. Used both to build a model. Purines, Pyrimidines and Sugars ...
The bacteria get into the developing flowers and transfer the T-DNA. ... survive on the selectable medium get transplanted and can be studies or screened. ...
The speed and magnitude of this genetic change is profound in many ... Remember Beadle & Tatum? Auxotrophic arg- mutants? Procedure requires: Replica plating ...
APOG. Homologous recombination. and plants. There is no gene replacement as in ... Tryptophan and Glutamine (CAG): the cyanide capsules within many proteins ...