Cation Exchanger

1
Cation Exchanger
-
-


Presented By Chen Liang, Adam Lis Jason
Mohan CHEE 450 Engineering Biology Monday,
April 2, 2007
-
-
-
-
-
-
2
-

Presentation Outline
What is a Cation Exchanger How Cation Exchangers
Work Process Options Production Target Design
Configuration Design Operation
Specifications Price Estimation
-
-
-
-
-
-
3
-

Unit Operations Specifications
Design Specifications
Design Objective
Human Insulin Capacity 4000kg/yr
- Purify supernatant to eliminate impurities -
Recover insulin precursor
-
-
-
-
-
-
4
-

Basic Parts of a Cation Exchanger
Resin Matrix
Column
Beads allow for counter-ions () to covalently
bind. Counter-ions can be reversibly exchanged
with other ions of the same charge. Beads used
to separate bulk mixture early in process. Fast
removal and high flow rate are essential.
Stainless Steel is suitable for larger column and
will last longer than glass. Design for even
input/output flow distribution. Coatings are
recommended for columns exposed to highly acidic
solutions.
-
-
-
-
-
-
5
-

How a Cation Exchanger Works
Chromatography Process
Starting Buffer Counter Ions Equilibrium is
established in column to allow for binding of
desired solute molecules. These counter-ions are
simple anions.
Adsorption of Sample Substances Solute molecules
with positive charge displace counter-ions and
bind reversibly to the resin.
Elution (Desorption) Substances are removed by
changing to elution conditions. This will cause
unfavourable ionic bonding of the solute
molecules. Elution can be changed by increasing
the ionic strength of the eluting buffer or
changing its pH. The most weakly bound
substances being eluted first.
6
-

Process Options

• Process Operation Steps
• Generating Equilibrium
• Injection of Supernatant
• Elution of Unwanted Material
• Elution of Target Protein
• Column Washing

Note Iso-electric Point 5.3
Signal/Concentration
Signal/Concentration
Time
Time
-
-
-
-
-
-
7
-

Cation Exchanger Target Operation
-
-
-
-
-
-
8
-

Process Design
Supernatant
Eluant
Wash
Impurities
Insulin Precursor
Column to meet purification method 4 Extra
columns for maintenance column failure 2
-
-
-
-
-
9
-

Design Illustration
Model No Chromaflow V1000 Bed Volume 236L Total
Binding Capacity 37.76kg Cross-Sectional Area
78.54cm2 Recommended Flowrate 523.60ml/min Estima
ted Price CDN 105000
Bead Specifications Model Name SP Sepharose XL
(strong) Average Bead Size 90µm
-
-
-
-
-
-
10
-

Unit Operation Cycle
-
-
-
-
-
-
11
-

Price Estimation
Total Capital Cost 3 462 000 Total Operating
Cost per Year 468 500
-
-
-
-
-
-
12
-

Questions
Thank You
-
-
-
-
-
-