Improving The Separation of Basic Drugs on HC Phases Catalyst

1
Ultimate Acid Stability and Superb Efficiency for
Basic Compounds
Secondary Crosslinking and Chloromethylation
Crosslink with Crosslinkers
Derivatization
Secondarily Crosslinked DM-CMPES
SH Crosslinked DM-CMPES
DM-CMPES
HC-C8
Styrene heptamer (SH)
(Chloromethyl)methylether (CH3OCH2Cl)
1-octylbenzene
a
AlCl3 is used to catalyze all three crosslinking
steps
2
SEM Showing Formation of Polymer Network
Coated Particles before HF digestion
After removal of all silica by HF digestion
Highly crosslinked polymer networks are formed
3
Acid Stability Comparison of the HC- C8 Phase and
the SB C18 Phase
The HC-C8 phase is MUCH MORE stable than the
best phase available after acid treatment
Hyper Crosslinked C8 After Acid Conditioning
47.5/47.5/5.0 ACN/Water/TFA pH 0.5 T 150 C
F 2.0 mL/min Solute Dodecylphenone(DDP)
SB C18
4
Silanophilicity comparison of ACE C18, ACE HC-C8,
Inertsil ODS and SB C18 in Formate Buffer
Chromatographic conditions For ACE HC-C8 and ACE
C18, 34/66 ACN/water, SB C18 38/62 ACN/water for
Inertsil ODS 16.3/83.7 ACN/water For all three
columns 40ºC, 0.1 formic acid ,1mL/min, ?254 nm
5
Overloading Study on ACE HC-C8, ACE C18, and SB
C18
Chromatographic conditions 35/65 ACN/water, For
all three columns 35ºC, 0.05 formic acid
,1mL/min, ?254 nm