Disk Stack Centrifuge

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Disk Stack Centrifuge

• Presented By
• Gureet Chandhok
• Waqar Khan
• Blake Mc Cord

• CHEE 450 Engineering Biology

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DISK STACK CENTRIFUGE

• A centrifuge is a piece of equipment, generally
  driven by a motor, that puts an object in
  rotation around a fixed axis, applying force
  perpendicular to the axis.
• Essentially works like a clarifier providing
  better control over residence time.

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Purpose of Centrifugation

• Since ours is a fermentation based bio process,
  the ISOLATION and the purification of the Insulin
  is the immediate goal once the fermentation step
  is concluded.
• In Insulin production, used to separate desired
  proteins from unwanted biomass.

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Types Of Centrifuges
Tricanter
Sedicanter
Decanter
Belt Press
Sorticanter
Disk Stack Centrifuge
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How does it Work?

• Disks are spaced out at 2mm.
• Spins rapidly exerting upto
• 12000 G force.
• Solid collects at the bottomliquids move up.

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Operating Factors

• Solid holding space
• Viscosity
• Diameter of particle
• Difference in Density

d2(?p- ?1)g vt -------------
18µ Stokes Law!
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Design Improvements

• Factors governing performance
• Volume of solids-Coagulation and Flocculation
• Settling Area-Residence Time

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CH-36B GOF

• Manufacturer-Alfa Laval
• Type Nozzle Centrifuge with continuous
  discharge
• Able to handle sensitive cell cultures
• Recirculation of solids
• Gentle gradual acceleration of product
• Minimization of shear forces preventing cell
  lysis
• No contact with oxygen
• No foaming or oxidation of product
• High grade stainless steel
• Steam sterilizable

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Design Specifications

• Feed Rate-3888.81 kg/h
• Viscosity of Liquid-1.5 cP
• Height-3.85m
• G-4300
• Speed- 2900 rpm
• Sedimentation Efficiency-30
• Motor Power-26 kW
• Volume- 18.5m3

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Cot

• Estimated to be 350,000-650,000
• Operating costs extra

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References

• http//www.flottweg.de/Broschu/DCbersichtsprospek
  t_E.pdf
• http//www.lsbu.ac.uk/biology/enztech/centrifugati
  on.html2_2
• http//www.lenntech.com/Centrifugation.htm
• www.fao.org/docrep/003/x6899e/X6899E12.jpg
• http//www.alfalaval.com/digitalassets/2/file10080
  _0_PD41493e.pdf
• http//www.alfalaval.com/ecoreJava/WebObjects/ecor
  eJava.woa/wa/showNode?siteNodeID8720contentID25
  790languageID1

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Questions?