PROJECT PROFILE

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PROJECT PROFILE
Title Referral
Centre for Virus Testing of Tissue Culture
Raised
Plants Duration Five
years Date of Start 28th
September 2006 Date of Termination 27th
September 2011 Project Cost (Rs.) 75.05
Lakhs Project Personnels Principal Investigator
R.K. Jain Co-Investigators Padma
Ramachandran/ V.K. Baranwal / Bikash
Mandal Research Associates Sudeep Bag /
Surekha Agarwal
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Major Objectives
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SERVICE APPROACH
Prepared write-up on Standard Operating
Procedures (SOPs) for virus testing of tissue
culture raised plants to be followed by
Accrediated Test Laboratories (ATLs)
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SERVICE APPROACH
Imparted training to personnel from Accredited
Test Laboratories and other Institutions
ATLs Technical undergoing training
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SERVICE APPROACH
Supplied Diagnostic Reagent to various
laboratories for virus detection
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Service Approach
Testing of plant samples for freedom from viruses
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SERVICE APPROACH

• Resource generation through testing of samples
  for freedom from viruses and other pathogens
• Tissue culture industries
• National Plant Quarantine Station

Rs. 1,40,000/-
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RESEARCH DEVELOPMENT APPROACH
Developed polyclonal antiserum to coat protein of
PRSV using recombinant technology
Amplification by RT-PCR
Cloning in pGEMT Easy Vector
850 bp
Amplification of coat protein gene, Lane 1
helathy Lane 2 infected sample
PRSV infected leaf
Mobilization of coat protein gene into expression
vector (pMAL-C2X)
Purification of expressed coat protein
850 bp
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Detection of Papaya ring spot virus (PRSV) in
infected papaya and other hosts samples collected
from different geographical locations by direct
antigen coated enzyme linked immunosorbent assay
(DAC-ELISA)
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RESEARCH DEVELOPMENT APPROACH
Developed polyclonal antiserum to coat protein of
CTV using recombinant technology
M 1 2 3 4
CTV infected material
700 bp
Cloning in pGEMT Easy
Amplification by RT-PCR
Mobilization of coat protein gene into expression
vector (pMAL-C2X)
M S IS FT W F4 F5 F8 F11MBP
M S IS FT W F4 F5 F8 F11MBP
Purification of expressed coat protein
M Marker S Soluble fraction IS Insoluble
fraction FT Flowthrough W Wash F4-11
eluted protein fraction
Validation of Antiserum at IIHR, Bangalore and
IARI, Regional Station, Kalimpong
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Developed repository of diagnostic reagents for
virus diagnosis
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Acknowledgements

• Department of Biotechnology DBT, New Delhi
• Indian Agricultural Research Institute (IARI) ,
  New Delhi
• Research Associates- Sudeep Bag and Surekha
  Agarwal