Observing Microorganisms Through a Microscope

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Chapter 3

• Observing Microorganisms Through a Microscope

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Units of measure

• Metric system
• Micrometer 10-6M
• Nanometer 10-9M

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Visible light

• Violet light short wavelength
• Red light long wavelength
• Interaction of light with objects
• Reflection
• Transmission
• Absorption with luminescence
• Fluorescence
• Phosphorescence
• Refraction

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Microscopes

• Light microscopy
• Compound light microscope

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Microscopes

• Light microscopy
• Compound light microscope
• Magnification vs. resolution
• Brightfield
• Darkfield
• Phase contrast

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Microscopes

• Light microscopy
• Compound light microscope
• Magnification vs. resolution
• Brightfield
• Darkfield
• Phase Contrast
• Fluorescent microscopy
• Fluorescent antibody technique

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Microscopes

• Electron microscopy
• Transmission electron microscope (TEM)
• Magnification 10,000 to 100,000 times
• Specimen is stained with heavy metal

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Microscopes

• Electron microscopy
• Transmission electron microscope (TEM)
• Scanning electron microscope (SEM)
• Magnification 1000 to 10,000 times
• Provides image of surface of specimen

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