In the name of God

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In the name of God
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Prevalence of Carriers of Methicillin Resistance
Staphylococcus aureus (MRSA) in 100 Staff of
Shahid Beheshti Hospital of Kashan (Iran)
A. Khorshidi, Gh. Shajari, A. Hosseini Negad
Department of Microbiology Immunology, Kashan
University of Medical Sciences Kashan, Iran
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Background
Staphylococcus aureus is a major cause of serious
hospital acquired infections in the past
decade, the prevalence of methicillin resistant
S.aureus (MRSA) strains has increased world wide
and they now represent a serious cause of
nosocomial infections in many countries (1). MRSA
has historically been considered to be a problem
associated with the hospital environment.
However, in recent years a dramatic increase in
the prevalence of MRSA has been reported that is
limited to an expanding reservoir of community
acquired MRSA (CA-MRSA) (2). The source of
infections is nasal carrier hospital personnel.
Determination of antibiotic resistance pattern of
isolated strains is essential for treatment of
carrier.
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Objective
The aim of this study was access the incidence of
methicillin resistant S.aureus (MRSA) carriage
in the Shahid Beheshti University Hospital of
Kashan (Iran).
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Materials and Methods
To find prevalence of MRSA carrier, A prospective
survey was conducted over 100 personnel in Shahid
Beheshti Hospital of Kashan, from March 2001 to
Sep 2002, total specimens were taken from the
anterior nares of staff, were cultured on the
selective media, isolates were identified based
on coagulase and conventional biochemical
reactions according in Standard Method.
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All staphylococcus isolates were screened for
methicillin resistance by in occulation of Muller
Hinton agar supplemented with salt and
oxacillin 6 µg/ml, according to National
committee for Clinical Laboratory Standards
(NCCLS) guidelines (3), other tested antibiotics
included ciprofloxacin, erythromycin, rifampin,
vancomycin, ceftriaxon, cloxacillin, cephazolin,
then the results were presented by descriptive
analysis.
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Results
The results showed (12) of Staphylococcal
isolates were coagulase positive, of the total
Staphylococcus aureus, 7 (58.3) were resistant
to methicillin. Prevalence of carrier of MRSA in
neonatal ward was (40), urgent ward (30),
Enternal ward (13.3). All of s.aureus were
susceptible (41.6) to cephazolin, ciprofloxacin,
ceftriaxon, erythromycin, rifampin and were
sensitive (33.3) to cloxacillin (table 1). All
of MRSA were sensitive (100) to vacomycin, (0)
to cloxacillin, (100) to cephazolin, (85.7) to
ceftriaxon, (71.4) to ciprofoloxacin, (42.8) to
erythromycin, (85.7) to rifampin (table 2).
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Table 1 Antimicrobial susceptibility results
for 12 S. aureus
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Table 2 Antimicrobial susceptibility results 7
MRSA
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Conclusion
Resistance pattern of Staphylococcus aureus to
various antibiotics, especially methicillin is to
wards increasing trend, it seems the prevalence
of MRSA clonisation in the staff of University
Hospital of Kashan (Iran) is increasing.
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References
1. Tiemersa EV. Bronzware SL, Lyytikaine o et al.
Mathicillin-resistanct Staphylococcus areus in
Europe. 1999-2000. Emerg Infect Dis 2004
101627-1634.
2. Caeletor HA. Diep BA. Charlebois ED.
Sensabaugh GF. Perdreau. Remington F. Community
adapted methicillin resistance Staphylococcus
(MRSA) Population dynamics of an expanding
community reservoir of MRSA. J Infect Dis 2004
1730-1739.
3. National Committee for clinical laboratory
Standards (NCCLS). Methods for dilution
antimicrobial saceptibility test for bacteria
that grow aerobically, 3rd ed. Approved standard
(M7-A3). Villanova, PA NCCLS, 1994.
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