Title: Biodegradation of the Prestige fuel oil under simulated conditions
1Biodegradation of the Prestige fuel oil under
simulated conditions
- S. DÍEZa, J. SABATÉb, M. VIÑASb, J. M. BAYONAa,
A. M . SOLANASb and J. ALBAIGÉSa - a Instituto Investigaciones Químicas y
Ambientales de Barcelona-CSIC. - B Universidad de Barcelona. Departamento de
Microbiología
2The fate of spilled oil in the marine environment
Fuente Albaigés 1989
3OBJECTIVES
- To evaluate the biodegradation extent of Prestige
fuel oil in vitro experiments times by two
consortia. - Aliphatics, aromatics and molecular markers.
- Effect of biodegradation on
- source and weathering indices
- To assess weathering effects on biodegradation.
- To evaluate the effect of oleophilic nutrients.
4METHODOLOGY
5Microbial Consortia Enrichment
Mineral medium
Inocula crude oil contaminated sand
5-PAHs (3-4 aromatic ring)
Inocula diesel contaminated Soil diesel oil
5 years
AM
TD
6Biodegradation Experiments
S200 microemulsion urea oleic acid
phosphate ester 2-butoxy-1-ethanol
7Chemical Analysis
Incubated fuel
pH lt 2.5 LLE (CH2Cl2)
surrogates
organic extract
Fractionation SPE (SiO2-C3CN)
Aliphatics GC-FID GC-MS
Aromatics GC-MS
8 HYDROCARBON BIODEGRADATION
9Biodegradation Aliphatics
10Biodegradation Aromatics
40
N1
original fuel
35
N2
30
N3
25
20
P1
P2
P
15
P3
N
10
5
0
40
35
biodegraded fuel 20 days
30
25
20
15
10
5
0
2
4
6
8
10
12
14
16
18
20
22
24
26
28
30
32
34
36
38
40
42
44
Time (min)
11Weathering Effect
12Strain Efficiency
13Oleophilic Nutrient Effect
14Alkylbenzenes
(II)
ORIGINAL FUEL
m/z 106 alkyltoluenes
(II)
BIODEGRADED (TD)
1-methyl-3-phytanylbenzene
m/z 106
ORIGINAL FUEL
m/z 92 alkylbenzenes
(I)
phytanylbenzene
(I)
BIODEGRADED (TD)
m/z 92
15C2 C3-Phenanthrenes
C2-Phenanthrenes(P2)
ORIGINAL
m/z 206
BIODEGRADED
m/z 206
C3-Phenanthrenes(P3)
ORIGINAL
m/z 220
BIODEGRADED
m/z 220
16C2 C3- Dibenzothiophenes
C2- Dibenzothiophenes (D2)
ORIGINAL
m/z 212
BIODEGRADED
m/z 212
ORIGINAL
C3- Dibenzothiophenes(D3)
m/z 226
BIODEGRADED
m/z 226
17Source Indices
Hopanes
Steranes
18BIODEGRADATION MOLECULAR MARKERS
Acyclic isoprenoids Diasteranes
C27-steranes bb-Steranes Homohopanes
Monoaroamtic steranes Triaromatic steranes
biodegradation
19Effect of Biodegradation on Source Diagnostic
Ratios
isoprenoides
hopanes
steranes
20Source Indices
D2/P2 D3/P3
21Effect of Biodegradation on Source Diagnostic
Ratios
22Weathering Indices
D2/C2 D3/C3
C2
256270
C3
D2
D3
23Effect of Biodegradation on Weathering
Diagnostic Ratios
24CONCLUSIONS
- N-alkanes, alkylbenzenes low MW parent PAHs and
mono and di-alkylated derivatives are degraded by
both consortia in 20-40 days incubation. - Degradation rates of PAHs decrease according with
the alkyl substitution and number aromatic rings. - Weathering slow down biodegradation.
- Oleophilic nutrient enhance the biodegradation of
the more refractory PAHs and some molecular
markers. - Source indicators can be modified when oleophilic
nutrient are added to the incubation medium.
25ACKNOWLEDGEMENTS
- Financial support was obtained from the MCYT
(VEM2003-20068-CO5-01). - Dr. S. Diez acknowledges a Ramon Cajal contract.