Fe Microbial Mats at Vailulu

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Fe Microbial Mats at Vailuluu
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Integrated microbiology and geochemistry
Geochemical Characterization Diffraction,
Electron Microscopy, Microprobe
Vailuluu
Biofilm Experiments Pure culture consortia,
surface chemistry
Molecular Characterization T-RFLP, 16s sequencing
Enrichment cultures Isolations Aerobic and
anaerobic Psychrophilic to Thermophilic
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Legend
Gamma-Proteobacteria
Alpha-Proteobacteria
2
Epsilon-Proteobacteria
4
Planctomycetes
8
Delta-Proteobacteria
Uncultured Bacteria
8
Bacteriodes
45
31
25
33
4
6
24
8
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Heterotrophs Mn-oxidizers
Submersible Collection of Weathered Pillow Basalts
Lithotrophic Fe-oxidizers
Simple to complex organics MnCl2
Fe/Mn oxides on glassy margin
Siderophore producers
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Isolates from Culturing
Legend
Gamma-Proteobacteria
Alpha-Proteobacteria
Bacteroides
Bacillus
Uncultured Bacteria
6
2
2
5
85

• Best Correspondence between Clone libraries and
  Isolates
• Pseudoalteromonas

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Fe-Oxides from Enrichment cultures
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Microbial interactions with the fresh basalt.