Molecular cytogenetics

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1. Molecular cytogenetics
2. Species and genomic relationships
3. Genomic changes in interspecific hybrids
4. Collecting wild species
5. Repetititive sequences
6. Retratransposons
7. In situ conservation
8. Landraces
9. Molecular markers

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RAPDs
AFLPs
Cv
r 0,92 Mantel
Theoretical and Applied Genetics (2004) Drossou
et al.
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Evolutionary Network
C
Ac
Al
Ap Ad
AC ACD
AC ACD
A.strigosa A. sativa
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PCOORDA Plot
0.61
0.36











0.11
AXIS2 9.9
7433
7041
AXIS2 - 9.9
AXIS2 - 9.9
















As
19437


Al



Ac
-
0.14


Ad





Ap



AB



AC




ACD





C



A. elatius





-
0.39


-
0.34
-
0.06
0.22
0.49
0.77






AXIS1 - 18.4
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Screening of C-genome Clones in Allopolyploids

• PCR Cloning of the full length ITS1-5.8S-ITS2
  region of A. insularis A, A. maroccana 2190, A.
  sativa cv Image, A. fatua L. N127, A. sterilis
  4704
• Two hundred individual ITS clones of each
  genotype were screened in bulked (groups of 10)
  SCARs reactions using the C-genome specific
  primers (CITS1F and CITS2R)
• Groups that gave strong positive signals were
  further analysed as individuals
• One to three putative C-genome clones for each
  accession were sequenced

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Nikoloudakis, N., Katsiotis, A. The origin of the
C-genome and cytoplasm of Avena polyploids (2008)
Theoretical and Applied Genetics, pp. 1-9.
Article in Press. Nikoloudakis, N., Skaracis,
G., Katsiotis, A. Evolutionary insights inferred
by molecular analysis of the ITS1-5.8S-ITS2 and
IGS Avena spp. sequences (2008) Molecular
Phylogenetics and Evolution, 46 (1), pp. 102-115.
Drossou, A., Katsiotis, A., Leggett, J.M.,
Loukas, M., Tsakas, S. Genome and species
relationships in genus Avena based on RAPD and
AFLP molecular markers (2004) Theoretical and
Applied Genetics, 109 (1), pp. 48-54.
Katsiotis, A., Loukas, M., Heslop-Harrison,
J.S. Repetitive DNA, genome and species
relationships in Avena and Arrhenatherum
(Poaceae) (2000) Annals of Botany, 86 (6), pp.
1135-1142. Katsiotis, A., Hagidimitriou, M.,
Heslop-Harrison, J.S. The close relationship
between the A and B genomes in Avena L. (Poaceae)
determined by molecular cytogenetic analysis of
total genomic, tandemly and dispersed repetitive
DNA sequences (1997) Annals of Botany, 79 (2),
pp. 103-109.