Detecting Protein Function and ProteinProtein Interactions from Genome Sequences

1
Detecting Protein Function and Protein-Protein
Interactions from Genome Sequences

• TuyetLinh Nguyen

2
Outline

• Putative Protein Interactions
• Domain Fusion Analysis
• Hypothesis for Evolution of Protein Interactions
• False predictions

3
Putative Protein Interactions

• 4290 protein sequences
• 6809 pairs of nonhomologous sequences
• Five candidates

4
Domain Fusion Analysis

• First method uses the annotation of proteins
  given in the SWISS-PROT database
• Second test of the interactions predicted by the
  domain fusion analysis uses as confirmation the
  Database of Interacting Proteins
• Third test of domain fusion predictions is by
  another computational method for predicting
  interactions.

5
Hypothesis for Evolution of Protein Interactions
6
False Predictions

• Physical interactions may be made by the domain
  fusion analysis
• The domain cannot distinguish between homologs
  that bind and those that do not

7
Conclusion

• Genomics information opens new paths to
  biochemical discoveries.