Jay R. Hove, Ph.D.

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IMAGING DYNAMIC INTRAVITAL MOTION

• Jay R. Hove, Ph.D.
• Dept. of Molecular Cellular Physiology
• University of Cincinnati College of Medicine

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Internal Flow Form and Function
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External Flow Visualization
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Internal Flow Visualization
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Planar Particle Image Velocimetry
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Quantitative Flow Visualization
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Rapid Development
Small Size
Ectothermic
Genetic Tractability
Optically Transparent
Highly Fecund
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Confocal Optical Dissection
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Visualizing Function
4.5 dpf zebrafish heart
2.5 dpf zebrafish heart

• Formation of heart tube 19hpf
• Heart tube contraction begins 22hpf
• Looping starts 33hpf
• Chambers begin to form 36hpf
• Four chambers visible
• Valves begin to form 48hpf
• Adult configuration attained 5dpf

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Erythrocytes as Flow Markers
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Synthetic Tracers
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Intravital Flow Mapping
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Quantifying Planar Microscale Flow
R2 0.8
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3D Through Image Stacking
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Pinhole Principle
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Defocusing Principle
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Mapping Complex Flows in 4D
A
B
BA
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Microscopic Imaging with Multiple Pinholes
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Microscale Defocusing Objective Masks
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Triangle Point Spread Z-Dimension
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In Vivo 4D Imaging
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Whats Next?

• Validate microscope-based spatial measurements
  in both static
• and dynamic paradigms

• Optimize pinhole spacing/diameter for all lenses
  and illumination

• Implement particle tracking velocimetry for low
  tracer density
• areas and higher spatial resolution

• Modify analysis algorithms to account for moving
  boundaries and
• large velocity gradients

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Acknowledgements
Collaborators Prof. Mory Gharib (Caltech)
Prof. Dana Dabiri (U Washington) Prof. Scott
Fraser (Caltech) Prof. Mary Dickinson (Baylor
U) Prof. Shmuel Einav (SUNY Stony Brook)
Prof. Malcolm Gordon (UCLA) Prof. Ian Bartol
(Old Dominion U) Dr. Jian Liu (Caltech) Dr.
Gabriel Acevedo-Bolton (UCSF) Dr. Arian
Forouhar (Caltech) Dr. Francisco Pereira
(INSEAN, Italy)
Hove Laboratory
NIH/NCRR Division of Comparative Medicine