Tin Shui Wai Government Secondary School

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Hong Kong Student Science Competition 2002
Fungi-New source of protein ??-?????
Tin Shui Wai Government Secondary School
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Why fungi may act as an alternative source of
protein from meat?

• Suitable for vegetarians
• Short of land for animal husbandry
• Higher production rate
• Relative low saturated fatty acid toxin
• Fit human digestive system more
• Contain cellulose, vitamins
• Can be use in medicinal aspect

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• Section 1 Consumer survey
• Part 1 Survey on price
• Part 2 65 people were interviewed through
  telephone

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Result
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• Section 1 Consumer survey
• Part 1 Survey on price
• Part 2 65 people were interviewed through
  telephone

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Understanding of fungi
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Section 2 Scientific research Part 1
Investigation on protein content Part 2
Identification of amino acids
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Kjeldahl Method

• Digestion
• Distillation
• Titration

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• Procedure
• 1.Ground and dried of sample

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2.Refluxed with conc. H2SO4
heat Protein H2SO4(l) ? CO2(g)H2O(l)
(NH4)2SO4(aq)
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3.Alkalization of mixture distillation of
ammonia (NH4)2SO4(aq)2NaOH(aq) ?2NH3(aq)
Na2SO4(aq)2H2O(l)
NH3 (aq) HCl (aq) ? NH4Cl (aq)
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• 4.Titration of excess HCl against NaOH
• NaOH(aq)HCl(aq)? NaCl(aq)H2O

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• Result

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Procedure 1.Digestion by reflux
2.Spotting of sample
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3.Developing of solvent
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• Solvent front was marked filter paper was dried
• Sprayed with ninhydrin dried in oven

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6.Rf values was calculated
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Result
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Social Effects ???, ??? and ??? have reasonable
high protein content with respect to meat
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What is the advantages for substitute meat with
fungi?

• Increase the total food supply
• Fungi recycle dead organic matter
• Suitable for developing countries
• Good way to get protein in places with
  geographical problem
• New source of protein for vegetarian

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Further investigation

• More foodstuff can be investigated
• Other groups of nutrients can be checked
• Environmental factor affect the growth of fungi
• Two dimensional paper chromatography
• Effects on cooking processing

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Conclusion ???, ??? and ??? contain high protein
content and reasonable prices, they were found to
be a good alternative sources of protein.
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Dishes of fungi
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Thank you!!!