Chem 371471

1
Chem 371/471

• BIOCHEMISTRY I Structure/Function and Mechanism
  of Enzyme Catalysis
• MWF
• 840 - 930

2
Logistics and Policies

• Textbook selection
• Lehninger Principles of Biochemistry
• Nelson and Cox (4th Edition 2005)
• Covered topics
• Scoring scheme
• Term paper
• Problem set

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Topics Covered in the Course

• Protein Structures
• Enzymes and Catatlytic Mechanisms
• DNA and RNA Structures
• Membrane Proteins
• Cell Signaling
• Protein Folding and Sequence analysis

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What is Biochemistry?

• Biochemistry is the study of the chemistry of
  life (Voet, Voet, and Pratt)
• Biochemistry seeks to explain life in chemical
  terms (Lehninger, Nelson, and Cox)
• Biochemistry seeks to describe the structure,
  organization, and functions of living matter in
  molecular term. (Mathews, Van Holde, Ahern)
• Biochemistry is the study of molecular basis of
  life (Stryer)

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Roots of Biochemistry

• 1828, Friedrich Wohler wrote to a friend
• I must tell you that I can prepare urea
  without requiring a kidney or an animal, either
  man or dog.

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Biochemistry studies

• What are the chemical and 3D structures of
  biological molecules?
• How do biological molecules interact with each
  other?
• How does the cell synthesize and degrade
  biological molecules?
• How is energy conserved and used by the cell?
• What are the mechanisms for organizing biological
  molecules and coordinating their activities?
• How is genetic information stored, transmitted
  and expressed?

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Movers of Modern Biochemistry

• 1901 Wilham Conrad Roentgen discovery of X-ray
• 1914 Max Von Laue X-ray diffraction by crystals
• 1915 Henry Bragg Lawrence Bragg The theory of
  X-ray diffraction
• 1958 Frederick Sanger Protein Sequencing
• 1962 Max Perutz, John Kendrew Structures of
  Myoglobin and Heamoglobin by X-ray
  crystallography
• 1962 Francis Crick, James Watson, Maurice
  Wilkins Structure of DNA
• 1964 Dorothy Crowfoot Hodgkin Structures of
  Penicillin and Vitamin B12
• 1980 Paul Berg, Walter Gilbert, Frederick Sanger
  Recombinant DNA and DNA sequencing
• 1982 Aaron Klug Crystallographic electron
  microscopy
• 1985 Herbert A. Hauptman, Jerome Karle Methods
  in X-ray crystallography
• 1988 Johann Deisenhofer, Robert Huber, Hartmut
  Michel Structure of membrane proteins

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Movers of Modern Biochemistry

• 1989 Sidney Altman, Thomas R. Cech Catalytic RNA
• 1993 Kary B. Mullis, Michael Smith PCR
• 1997 Paul D. Boyer, John E. Walker, Jens C. Skou
  Mechanism of ATP synthesis

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Schematic diagram of an animal cell accompanied
by electron micrographs of its organelles.
Page 7
10
Simulated cross section of an E. coli cell
magnified around one millionfold.
Page 15