Title: INNATE NONSPECIFIC IMMUNITY
1INNATE ( NON-SPECIFIC ) IMMUNITY
Abdul Ghaffar Microbiology and Immunology
2Components of the Immune System
macrophages, neutrophils
complement, interferon, TNF etc.
T cells other effectors cells
antibodies
3Balance between Infection and Immunity
infection
immunity
4Response to Infection
adaptive immunity
5Significance of the Immune System
- Beneficial
- Protection from Invaders
- Elimination of Altered Self
- Detrimental
- Discomfort (inflammation)
- Damage to self (autoimmunity)
6Characteristics of Innate and Adaptive Immunity
Innate Immunity
Adaptive Immunity
7Components of Innate and Adaptive Immunity
Innate Immunity
Adaptive Immunity
physical barriers
skin, gut Villi, lung cilia,etc
none
soluble factors
many protein and non-protein secretions
Immunoglobulins (antibody)
cells
phagocytes, NK cell eosinophils, K cells
T and B lymphocytes
8Effector mechanisms in Innate Immunity -1
Site Component Functions
9Effector mechanisms in Innate Immunity -2
Site Component Functions
10Effector mechanisms in Innate Immunity -3
Site Component s Functions
Serum and other serous fluids
11Phagocytes are the Most Important Cells
12All phagocytes eat, digest and extrude
13Phagocytes Neutrophils
- phagocytosis, intracellular killing, inflammation
and tissue damage - characteristic nucleus, cytoplasm
- granules and CD67 membrane marker.
14PhagocytesMacrophages
- phagocytosis, intracellular and extra-cellular
killing, tissue repair, antigen presentation for
specific immune response - characteristic nucleus and CD14 membrane marker.
15Characteristics of Neutrophil Granules
primary granules
secondary granules
azurophilic characteristic of young neutrophils
specific for mature neutrophils
contain cationic proteins, lysozyme, defensins,
proteases and myeloperoxidase
contain lysozyme, NADPH oxidase, lactoferrin and
B12-binding protein
16Phagocyte Response to Infection
- The SOS Signals
- N-formyl methionine
- Clotting system peptides
- Complement products
- Phagocyte response
- Vascular adherence
- Diapedesis
- Chemotaxis
- Activation
- Phagocytosis and killing
17Initiation of Phagocytosis
Attachment via
18Respiratory Burst
Oxygen Dependent Myeloperoxidase Independent
Reactions
19Respiratory Burst
Oxygen Dependent Myeloperoxidase dependent
reactions
20Pathways of Intracellular Killing
21Mediators of Oxygen Independent Killing in the
Phago-lysosome
Effector Molecule
Function
Cationic proteins (cathepsin)
Damage to microbial membranes
Lysozyme
Hydrolyses mucopeptides in the cell wall
Lactoferrin
Deprives pathogens of iron
Hydrolytic enzymes (proteases)
Digests killed organisms
22Nitric Oxide Dependent Killing
23Non-specific Killer Cells
- NK and LAK cells
- K cell
- Activated
- macrophages
- Eosinophils
- They all kill foreign and altered self targets
24Natural Killer (NK) cells
- also known as large granular lymphocytes (LGL)
- kill infected and malignant cells
- are identified by the presence of CD56 CD16 and
absence of CD3 - activated by IL2 and IFN-? to become LAK cells
25Lymphokine Activated Killer (LAK) cell
IFN
IFN
IL2
IL2
26Regulation of NK Cell Function
27K Cells
- morphologically undefined
- have IgG Fc receptor
- recognize antibody coated targets
- could be NK cells (IgG), macrophages (IgG),
eosinophils (IgE) or other cells (IgG)
28Macrophages
- phagocytose kill intracellularly
- identified by CD14
- adherent of plastic and glass surfaces
- activated by cytokines
- kill both intracellular organisms
- kill malignant and altered self targets
29Elements of Nonspecific ImmunitySummary
- Physical Barriers
- skin, muco-ciliary escalator, secretions,
peristaltic movement, etc. - Cells
- pagocytes
- Oxygen-independent killing
- O2-dependent-MPO independent killing
- O2-dependent-MPO dependent killing
- Nitric Oxide mediated killing
- cytotoxic cells
- NK, LAK, K (ADCC)