What is SV40 Tantigen What does it do

1
What is SV40 T-antigen?What does it do?

• Presented by ???
• Genome Engineering Laboratory,
• Institute of Bioinformatics,
• National Yang-Ming University
• 2003/12/12

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Simian Virus 40

• Hosts monkeys and mice
• DNA virus with 5,227 base pairs

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SV40 T-antigen
AUG
UUA
UUA
Early pre-mRNA
Large T mRNA
AAAn
Small T mRNA
AAAn
Large T 94kd protein Small T 17kd protein
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Large T-antigen

• Delete 1-126 a.a.
• Delete 136-C terminus
• Addition 127-132 a.a toß-galactosidase and
  pyruvate kinase

• Delete 127-132 a.a.

Immunofluorescence microscopy Daniel Kalderon,
Bruce L. Roberts, Alan E. Smith, A Short Amino
Acid Sequence Able to Specify Nuclear Location,
Cell 39499-509, 1984
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Tumor Virus

• Human cells are semipermissive cell

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Tumor Virus

• Protein binding sites

Rb-binding
P53-binding
SV40 Large T
Release transcription factor for Rb
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THANKS!
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Hahn WC, et al,. Creation of human tumor cells
with defined genetic elements. Nature 400,
464468 (1999)