A MICROFLUIDIC CHIP WITH A NANOSCALE ARRAY FOR ANALYSIS OF VIRUS PARTICLES

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MicroTAS 2006
A MICROFLUIDIC CHIP WITH A NANOSCALE ARRAY FOR
ANALYSIS OF VIRUS PARTICLES
Kidong Park, Demir Akin, Rashid Bashir Birck
Nanotechnology Center, School of Electrical and
Computer Engineering, Weldon School of Biomedical
Engineering, Purdue University, West Lafayette,
IN. USA
Reporter Tzu-Yu Chao
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OUTLINE
Introduction Method Fabrication Experiment
results Conclusion Reference
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Introduction
Ref A Scalable Addressable Positive-Dielectrophor
etic Cell-Sorting Array Brian M. Taff, et al.
Department of Electrical Engineering and Computer
Science, Massachusetts Institute of Technology,
Cambridge
Ref Manipulation of herpes simplex virus type 1
by dielectrophoresis Michael P. Hughes, et al.
Bioelectronics Research Centre, Department of
Electronics and Electrical Engineering,
University of Glasgow, Glasgow G12 8QQ, UK
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• Nonmechanical filters
• Sample preparation
• Purification
• Concentration of viral particles from a mixed
  sample
• Real-time imaging of nanometer scale virus
  particles for analysis
• Capture
• Detection
• Characterization of these particles within micro
  and nanoscale sensors

Ref Real-Time Virus Trapping and Fluorescent
Imaging in Microfluidic Devices Demir Akin, et
al. Laboratory of Integrated Biomedical
Micro/Nanotechnology and Applications (LIBNA)
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Method
DEP force
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Fabrication
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SEM images of the probe. The gap between each
probe pair is about 1-2 µm.
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Experiment Result
1. polystyrene beads 2. lambda virus 3.
vaccinia virus
The collected 3um polystyrene beads. The beads
are collected between the electrodes. (vfluid1
mm/s, VDEP20 Vpp _at_ 100 kHz)
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Lambda virus captured between the probe tips.
Capsid labeled by green (DiOC63, Molecular
Probes) DNA labeled by red (DiL, Molecular
Probes)
SEM images of the captured lambda virus. (a) The
probe pair with captured virus. (b) The
magnified image of the lower probe.
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The vaccinia viruses(????) were captured with
positive DEP
Capsid labeled by green (DiOC63) DNA labeled by
blue (Hoescht33342, Molecular Probes)
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Conclusion

• The nano-scale probe array, integrated with
  micro-fluidic channel applying high electric
  filed, produced by its ultra sharp probe tips,
  the effect of the electric field on virus
  particle can be studied.
• The capture of the virus particles is shown with
  colocalization of the two different fluorescence
  signals in real-time.

References
1. B.M. Taff J. Voldman, A Scalable
Addressable Positive-Dielectrophoretic
Cell-Sorting Array, Analytical Chemistry 77,
7976-7983 (2005). 2. D. Akin, H. Li, R. Bashir,
Real-Time Virus Trapping and Fluorescent Imaging
in Micro-fluidic Devices, Nano Letters, 4 (2),
257 -259, 2004. 3. Ying Huang, et al,
Differences in the AC electrodyamics of viable
and non-viable yeast cells determined through
combined dielectrophoresis and electrorotation
studies, Phys. Med. Biol., 1992, Vol. 37, No 7,
14991517. 4. M. P. Hughes, Hywel Morgan,
Frazer J. Rixon, Measuring the dielectric
properties of herpes simplex virus type 1 virions
with dielectrophoresis, Biochimica et Biophysica
Acta 1571 (2002)1-8