CLINICAL PNEUMOCOCCALISOLATES FROM THREE HOSPITALS IN NAIROBI

1
CLINICAL PNEUMOCOCCALISOLATES FROM THREE
HOSPITALS IN NAIROBI

• (In the past 3 years)
• The Aga Khan Hospital
• Getrude Garden Childrens Hospital
• Kenyatta National Hospital

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CLINICAL PNEUMOCOCCALISOLATES - 3 HOSPITALS IN
NAIROBI
3
ANTIBIOTIC SUSCEPTIBILITY OF ST. PNEMONIAE 104
ISOLATES (E test)
4
PNEUMOCOCCAL ANTIGEN DETECTION IN URINE

• A study done between June 2001 October 2002
• The Aga Khan Hospital and Getrudes Garden
  Children Hospital were involved .
• A rapid testing kit manufactured by Binax of
  USA was used.
• Test is based on immunochromatography and takes
  only a few minutes to complete.
• Requires only a few drops of urine.
• All other requirements are provided in the kit.
• Expensive 20USD/test.

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EVALUATION OF THE KIT

• 100 urine samples from well baby clinic (with no
  H/O fever in the past 8 weeks) were tested. One
  sample tested positive 1 false positive.
• 100 samples from healthy blood bank donors were
  tested after they were found fit to donate. All
  tested negative.

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PNEUMOCOCCAL ANTIGEN DETECTION IN URINE CONTD

• 150 urine samples from children admitted with
  febrile illness were tested. 21 samples tested
  positive. All patients had urine, sputum/throat
  swab, and blood cultures performed. There were 8
  culture isolations of pneumococci from these 21
  patients which were all from respiratory
  specimens.

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PNEUMOCOCCAL ANTIGEN DETECTION IN URINE CONTD.

• 200 samples from adult patients admitted with a
  diagnosis of pneumonia, febrile illness and
  meningitis were tested. Patients were mostly
  from medical wards and 30 from I.C.U.
• 43 samples tested positive . There were 16
  culture isolations of pneumococci from these
  patients.

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CONCLUSION

• A large number of pneumococcal infections are not
  diagnosed by routine clinical microbiology
  procedures.
• Diagnosis could be improved tremendously if
  antigen detection tests were used in addition to
  culture techniques.