DNA Fingerprinting and Cloning

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DNA Fingerprinting and Cloning
Heather Janee Paige Amber Trae
Jerricka Chris Casey Courtney Kara
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Day 1
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DNA Background
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Breaking Down Chromosomes into DNA
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Growing and Viewing DNA
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• There are 6,000 known single-gene genetic
  disorders
• They occur about 1 in every 200 births
• Cystic fibrosis, sickle cell anemia, Marfan
  syndrome, Huntingtons disease, and hereditary
  hemochromatosis are just a few of the known
  diseases.

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PlasmidsAntibiotic Resistance
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Two types of Plasmids
pUC18
LUX
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Learning About Plasmids
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Separation of Plasmids and DNA
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Gummy Bear Game

• Twizzlers Plasmid
• Gummy Bears nucleotides
• Little Twizzlers restriction enzyme
• The object of the game was to get an outlook of
  what plasmids look like due to the small size of
  the DNA.

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Sequence of DNA Strands
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Fitness Tips from Emily

• Purpose of the Cardiac Project is to reduce
  cardiovascular disease mortality in WV through
  research and intervention with children.

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Screening Done for Cardiac Project

• Check height and weight
• Calculate Body Mass Index (BMI)
• Check blood pressure
• Measure blood cholesterol
• Examine neck for Acanthosis Nigricans (AN)

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Dance Get Fit
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(No Transcript)
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Day 2
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Restriction Enzymes
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Restriction Enzymes

• Restriction enzymes are DNA-cutting enzymes
  found in bacteria.
• A restriction enzyme cuts the DNA
• It also allows the double helix form a single
  strand
• EcoR1 and BamH1 are enzymes we use in
  fingerprinting.

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Restriction Enzymes follow the chain of DNA until
they find the pattern they recognize and cut it
in half so scientist can insert new DNA.
Heather and Kara are labeling that contain horse
and sheep DNA that are going to be split by the
enzyme.
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Hard at Work
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Cytogenetics The Newest Science
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Visit the Clinical Geneticist
Cindy Cutenese
Chromosomal Chart
Janee, Casey, Amber, and Courtney studying
Genetic Diseases
Heather C.C. looking at Karyotypes
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Cytogenetics Tools
Amniocentesis
Karyotype
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Day 3
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DNA Fingerprinting
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DNA Fingerprinting
DNA 1
DNA 2
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Looking at DNA
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Day 4
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Relay Races
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And the competition begins
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The Winners
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Individual Competition
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Adios!