Oxidative Stress

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• 2008/05/02
• ???

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Oxidative Stress

• Reactive oxygen species (ROS)
• Oxygen ions
• Free radicals
• Peroxides
• Antioxidant defense
• Damage sperm membrane and motility
• Alter sperm DNA and initiate apoptosis

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Male infertility

• Half of all cases of infertility
• Affects one man in 20
• Reduction in seminal plasma and spermatozoa
  glutathione peroxidase
• Reduction in non-enzymatic antioxidant activity
  in seminal plasma
• IUI or ICSI, not for oxidative stress

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Leukocyte

• Positive correlation between seminal leukocytes
  number and ROS production
• No significance difference between fertile and
  infertile men
• Positive correlation between seminal ROS and
  cytokines (IL-6, IL-8, TNF-a)

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Sperm

• ROS assists the sperms transit through the
  cumulus and zona pellicida
• ROS production ability inversely correlates with
  maturation state
• Excess cytoplasmic residues in the mid- piece are
  rich in G6PD ? NADPH production
• Produce less ROS (1/1000) but cause more DNA
  damage

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Identification of oxidative stress

• The cost and complexity of testing
• The lack of a single standardized measure of
  oxidative stress
• Direct method damage created by ROS
• Indirect method ROS production
• Routine semen analysis asthenozoospermia,
  hyperviscosity of seminal plasma, seminal
  leukocytes, poor sperm membrane integrity

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