Outline for lecture 4

1
Outline for lecture 4

• Assignment 1
• Real-time analysis of Honeybee DNA sequence
• Honeybee paper

2
Assignment 1 13.5 marks

• Page 1(3.5) Picture of the agarose gel with your
  lane clearly indicated.

3
Assignment 1 13.5 marks

• Pages 2-5(10) Top of page 2 will be your clipped
  sequence. Three page maximum description of your
  analysis and conclusions (2-4). 12 point font
  minimum 1.5 line spacing minimum 1 inch
  margins figures (1page max) included. This must
  be individual work no collaboration. Plagiarism
  is a serious academic offence.
• Page 5 references.
• Expectations are on the web site.

4
Assignment 1

• Due Monday Oct. 20

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Page 1 Example gel
My lane
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T7 sequencing primer?
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Tonys clone Bio396X
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Example sequence

• GGTAANGGAACTGGAATCCAAACTCTCTGAAGCTGAGAAGGAATTCATCG
  A
• AGGAGCACCAACACGTAGCAAACGATCACCATCCGAGTGGATACCAAGGC
  CACCCGAAAAATACAGTCTTACTGGGCACA
• GAGCTCCTATCAACAGAGTTATTTTCCATCCGGTCTTTAGTCTTATAGTA
  TCTGCCAGCGAAGATGCCACTATCAAGGTG
• TGGGACTTCGAGAGCGGCGAATTCGAAAGAACGTTGAAGGGGCACACCGA
  CAGCGTGCAGGACGTTTCCTTCGACGTCTC
• CGGGAAACTGTTAGTCTCATGCAGTGCGGACATGTCTATTAAGTTATGGG
  ACTTTCACCAGTCATTCGCCTGCGTGAAAA
• CCATGCACGGACATGATCACAGTGTCAGCTCTGTCGCATTTGTGCCACAA
  GGGGATTTCGTAGTGAGCGCCTCTAGGGAT
• AAGACCATCAAAATATGGGAAGTAGCGACAGGGTATTGTGTCAAAACGTT
  AACGGGGCACAGAGAATGGGTACGGATGGC
• CAGAGTCAGTCCTTGTGGAGAATTAATAGCTAGTTGCTCGAACGATCAAA
  CAGTACGGGTTTGGCACGTGGCAACAAAGG
• AAACGAAGGTCGAACTCAGAGACCACGAACACGTAGTGGAGTGTATCGCA
  TGGGCACCGGACAGTGCAAGAGCATCGATC
• AACGCTGCTGCAGGGGCGGACAATAAGGGAGCCCATGAAGGACCTTTCCT
  CGCATCTGGCTCGCGAGACAAAGTAATTCG
• TGTATGGGATGTCGGTGCCGGTGTTTGTCTCTTCGCCCTATTGGGCCACG
  ACAACTGGGTTCGCGGCATCGTCTTCCATC
• CTGGTGGCAAGTTCATCGTCAGTGNCTCTGACGACAAGANCCTGCGAGTA
  TNGGANACGCGCAACANANGGGTAATGAAA
• ACCCTCNAAGCGCACGTCCACTTCTGCNCCTCCNTTGATTTCACAAAAGC
  CATCCTTACGTGGTCNCCGGTAGTG

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Step into liquid
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Taken from Vallee et al., 2001
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Taken from Tarricone et al., Neuron 2004
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Discussion of Honey Bee paper