Prsentation PowerPoint

1
Use of Near-infrared microscopy (NIRM) and IR
Camera to detect and quantify meat and bone meal
(MBM) in compound feeds
BAETEN Vincent, MICHOTTE RENIER
Antoine, VERMEULEN Philippe and DARDENNE
Pierre Contact baeten_at_cra.wallonie.be
Agricultural Research Centre of Gembloux CRAGx
Chaussée de Namur, 24 5030 GEMBLOUX (Belgium)
2
UE Reference method Classical microscopic
? Classical microscopy is based on the visual
observation of morphologic features of
ingredient particles
? Infrared spectroscopy methods are based on the
organic composition of ingredient particles
Our objective gt replace the eyes of the
analyst (i.e. visible detectors) by infrared
detectors and the expertise of the microscopist
by discriminant equations
Source STRATFEED Decision Support System
(RIKILT, NL)
3
METHOD OF REFERENCE CLASSICAL MICROSCOPY
Grinding (1-2 mm)
4
Infrared region of the electromagnetic spectrum
5
Near-infrared spectral information
Log (1/R)
Fat
Protein
Fat
Water
Wavelength (nm)
6
Near-infrared microscopy NIRM  Twinning the
Microscopy and Spectroscopy advantages 
7
NIRM Instrument microscope coupled to a FT-NIR
9000-4000 / 8 cm-1 1112 2500 / /- 2
nm /- 5 nm -gt 4 nm 2 sec/spectrum 10
scans 20 sec/particle
8
NIR microscopy NIR particles spectra
Sample holder with particles
9
FILTERING OF THE RESULTS OF EACH EQUATION
10
 NIRM results for samples spiked at 0.05, 0.1,
0.5 and 1
LOD depend on the number of particles analysed
11
Detection of MBM in FISHMEAL
In collaboration with Ian Murray, SAC
12
NIRM CONCLUSION
Conditions to find at least one particule with
95 of probability
13
Near-infrared Camera A screening method for
the control laboratory at very low level 
14
Near-infrared camera
76 800 pixels (240 x 320) 4 cm² Area 70
µm² Range from 900 nm to 1700 nm Minimum
resolution 6 nm Acquisition time less than 5
minutes
15
ARTIFICIAL model
16
PLS discrimination of MBM particles
PLS (-1.3 lt lt -0.7 , 0.7lt lt1.3 , wrong
attribution and out of the confidence
interval )
17
European Project G6RD-2000-CT-00414
Strategies and methods to detect and
quantify Mammalian tissues in feedingstuffs Proj
ect coordinator Dr Pierre DARDENNE Agricultural
Research Centre of Gembloux (CRAGx) Chaussée de
Namur, 24 5030 Gembloux Belgium Tél [phone]
03 50, Fax [phone] Web Site
www.stratfeed.cra.wallonie.be
18
(No Transcript)
19
STRATFEED - MICROSCOPY PICTURES LIBRARY
20
STRATFEED - Decision support System
Source RIKILT, Leo van Raamsdonck
21
STRATFEED - Real-time PCR results (ISS,
Gianfranco BRAMBILLA)
Detection of cattle DNA in a blank feed matrice
adulterated with
5 MBM
3 MBM
1 MBM
0.5 MBM
Source CRAGx, Gilbert BERBEN
22
EUROPEAN STRATFEED NIRS NETWORK
KIT of 30 Ring Cups Sealed (ground material)
23
www.stratfeed.cra.wallonie.be
24
PROJECTS FUNDINGS
- Near-infrared microscopy
DG4 Ministry of Agriculture
EU Stratfeed project

-
NIR Camera Ministry of
Agriculture FNRS (Fonds
National de Recherche Scientifique)
CRAGx (Centre de Recherches Agronomiques
de
Gembloux) - STRATFEED EU project n
G6RD-2000-CT-00414 www.stratfeed.cra.wallonie.b
e Acknowledgement Isabelle Fissiaux and Félix
Rwagasore