The Citric Acid Cycle

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The Citric Acid Cycle

• Mary Gearing

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Problems with this pathway

• Where does the oxaloacetate come from?
• How do we convert oxaloacetate to malate?

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Where in the world is 1.1.1.37?

• BLAST our genome against 1.1.1.37 (H. salinarium)

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Mistaken identity???
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Different BLAST result
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Can our organism convert pyruvate to
oxaloacetate? (Enzyme 6.4.1.1)
Pyruvate
6.4.1.1
Oxaloacetate
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BLASTing our genome
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Both correspond to EC number 2.3.3.13
(2-isopropylmalate synthase)
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So where does the oxaloacetate come from anyway?

• Aspartate metabolism
• Glycoxylate metabolism

L-aspartate
Oxaloacetate
2.6.1.1
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Unanswered Questions

• Oxaloacetate/malate conversion
• Pyruvate/oxaloacetate conversion
• We still dont know if these processes occur in
  H. utahensis.