SpotQC Titration Experiment

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• SpotQC Titration Experiment
• Eight different organism-specifc microarrays were
  hybridized with SpotQC at six different
  concentrations to determine the most ideal
  dilution to use for quality control.
• Dilutions were as follows
• 2 µM (SpotQC protocol)
• 1 µM
• .5 µM
• .25 µM
• .1 µM
• .01 µM

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• SpotQC Titration Experiment
• Tested 8 different organism print lots
• QAF01122005A A. fumigatus
• QBA12012004A B. Anthracis
• QLM11222004A L. monocytogenes
• 3QSA12292004A S. aureus
• QSM01042005A S. mutans
• 2QSP11012004A S. pneumoniae
• QVC12032004A V. cholera
• QYP12102004A Y. pestis

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• SpotQC Titration Experiment
• Print plates for each print lot varied in age
• Date Created
• QAF01122005A 7/30/04
• QBA12012004A 3/31/04
• QLM11222004A 5/20/04
• 3QSA12292004A 12/22/04
• QSM01042005A 4/8/04 (refreshed 12/3/04)
• 2QSP11012004A 4/8/04
• QVC12032004A 3/11/04
• QYP12102004A 3/11/04

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• SpotQC Titration Experiment
• Plates tested were 70mers or Amplicons
• QAF01122005A 70mer
• QBA12012004A 70mer
• QLM11222004A 70mer
• 3QSA12292004A 70mer
• QSM01042005A 70mer
• 2QSP11012004A Amplicon
• QVC12032004A 70mer
• QYP12102004A 70mer

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• SpotQC Titration Experiment
• Slides were hybridized according to SpotQC
  protocol.
• All slides were scanned on same scanner at PMT
  of 650.
• Spotfinder was used to generate data from each
  tif image.
• Log2 of signal intensities were calculated.
• Box plots of data were created using Origin 7.5.
  
• Data is visually represented as varying print
  lots across fixed concentrations as well as
  fixed print lots across varying concentrations.
  

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Each chart below graphs the concentration of
SpotQC across 8 different organism print lots.
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QBA 0.5uM
QBA 2uM
QBA 1uM
QBA 0.25uM
QBA 0.1
QBA 0.01uM
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Each chart below graphs an organism print lot at
different SpotQC concentrations.
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