Soybean SE Transformation

1

• Soybean SE Transformation
• Induction
• Plant and zygotic embryo production (6-8 weeks)
• Induction on D40 (6-8 weeks)
• Proliferation on solid D20 (4 weeks)
• Proliferation on FNL (4 weeks)

2
Soybean SE Transformation- cont. II. Gene
Transfer, Selection, Proliferation
Maturation Place 20-25 SE clumps on
D20 Shoot Continue on D20 (1 week) Transfer
into FNL/H20 for seln. (710 weeks) Isolate
green, well formed globular SEs, grow on
multi-well plate (5 wks) Proliferate selected
material in FNL (5 weeks) Mature healthy green
embryos matured on SHaM (4-5 weeks) Desiccation
(5-7 days) Select healthy matured SEs for
lipid and/or other analysis
3
Soybean SE Transformation- cont. III. Conversion
Seed Production Desiccated
SEs Germination (1 week) Plant hardening (3
weeks) Transfer hardy plantlets into pots,
grow-out in greenhouse Harvest mature seeds
(10-16 weeks)